FastQCFastQC Report
Thu 2 Feb 2017
SRR4062149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434202
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCA18130.1264117606864305No Hit
GCGTTAGCATCCGCAAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT17870.12459890587239456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17130.1194392421709076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.033.129081
GTATCAA32050.026.5033661
CGTCGTA3400.021.99836210
ACCGTCG3250.020.9830538
TACCGTC3650.020.4916257
CGCAATA2350.019.6608536
ATACCGT3950.018.9352996
GTATTAG2050.018.2489051
GTCTAAT851.4280656E-418.1226161
CTAGCGG2550.018.11882429
GTCGTAG4000.017.59868811
TATCAAC48650.017.5902582
ATCAACG49100.017.4284363
TCAACGC49850.017.1662244
CAACGCA50850.016.8718995
CCGTCGT4250.016.5634739
GTATAAT1206.3915268E-616.5045241
TAGGGCG800.0019877316.50107431
AACGCAG52150.016.4091346
CGCGCAT1906.166374E-1016.2115840
Produced by FastQC (version 0.11.5)