##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434202
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29520667242132	32.0	32.0	32.0	32.0	32.0
2	31.48992052723396	32.0	32.0	32.0	32.0	32.0
3	31.598191886498554	32.0	32.0	32.0	32.0	32.0
4	31.670463435415652	32.0	32.0	32.0	32.0	32.0
5	31.638784494792226	32.0	32.0	32.0	32.0	32.0
6	35.292133186259676	36.0	36.0	36.0	36.0	36.0
7	35.29496960679179	36.0	36.0	36.0	36.0	36.0
8	35.25267430947663	36.0	36.0	36.0	36.0	36.0
9	35.32626296714131	36.0	36.0	36.0	36.0	36.0
10	35.22272734245246	36.0	36.0	36.0	36.0	36.0
11	35.33536140655222	36.0	36.0	36.0	36.0	36.0
12	35.262804681627834	36.0	36.0	36.0	36.0	36.0
13	35.28995148521617	36.0	36.0	36.0	36.0	36.0
14	35.2588170982888	36.0	36.0	36.0	36.0	36.0
15	35.23821609508284	36.0	36.0	36.0	36.0	36.0
16	35.24597302193136	36.0	36.0	36.0	36.0	36.0
17	35.22404235944448	36.0	36.0	36.0	36.0	36.0
18	35.22638930917681	36.0	36.0	36.0	36.0	36.0
19	35.21493694751506	36.0	36.0	36.0	36.0	36.0
20	35.21436520099679	36.0	36.0	36.0	36.0	36.0
21	35.207466591177536	36.0	36.0	36.0	36.0	36.0
22	35.19160271705101	36.0	36.0	36.0	36.0	36.0
23	35.155982211710764	36.0	36.0	36.0	36.0	36.0
24	35.143759386753054	36.0	36.0	36.0	36.0	36.0
25	35.123212071939655	36.0	36.0	36.0	36.0	36.0
26	35.08727919776991	36.0	36.0	36.0	36.0	36.0
27	35.075438466826846	36.0	36.0	36.0	36.0	36.0
28	35.06401120623176	36.0	36.0	36.0	36.0	36.0
29	35.041797459493154	36.0	36.0	36.0	36.0	36.0
30	35.026269660759084	36.0	36.0	36.0	36.0	36.0
31	35.02387041713789	36.0	36.0	36.0	36.0	36.0
32	35.002477335828566	36.0	36.0	36.0	36.0	36.0
33	34.979268610697794	36.0	36.0	36.0	36.0	36.0
34	34.966342258621864	36.0	36.0	36.0	36.0	36.0
35	34.955202963041465	36.0	36.0	36.0	36.0	36.0
36	34.93224873483651	36.0	36.0	36.0	36.0	36.0
37	34.925117242898835	36.0	36.0	36.0	36.0	36.0
38	34.9081224262691	36.0	36.0	36.0	36.0	36.0
39	34.888763925862605	36.0	36.0	36.0	32.0	36.0
40	34.878636342718806	36.0	36.0	36.0	32.0	36.0
41	34.83835331424723	36.0	36.0	36.0	32.0	36.0
42	34.81980502049223	36.0	36.0	36.0	32.0	36.0
43	34.81168343092535	36.0	36.0	36.0	32.0	36.0
44	34.76271264438343	36.0	36.0	36.0	32.0	36.0
45	34.744086258421056	36.0	36.0	36.0	32.0	36.0
46	34.72145206881597	36.0	36.0	36.0	32.0	36.0
47	34.69662641664145	36.0	36.0	36.0	32.0	36.0
48	34.6719911142224	36.0	36.0	36.0	32.0	36.0
49	34.65433321108184	36.0	36.0	36.0	32.0	36.0
50	34.25886451141471	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	8.0
20	16.0
21	35.0
22	110.0
23	279.0
24	651.0
25	1629.0
26	3214.0
27	6196.0
28	10674.0
29	16849.0
30	25442.0
31	37994.0
32	57122.0
33	97132.0
34	224361.0
35	952489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.653058947023354	17.994622474707846	12.537864125071055	24.814454453197747
2	16.248804148037408	19.825063941472283	37.18123397976744	26.744897930722868
3	18.72603186733194	23.386552465632313	28.413968523130727	29.47344714390502
4	12.643825625678948	15.63154980958052	34.623435192532156	37.101189372208374
5	14.620464899644542	36.84676217157695	32.343351912771006	16.189421016007508
6	34.65013993844661	34.84306952577113	16.35920184186049	14.147588693921776
7	31.421584965018873	30.47757568320223	19.21221696804216	18.888622383736738
8	29.1225364348955	32.33972620314293	18.280270143257365	20.2574672187042
9	27.282070447538075	14.528218479684174	18.5393689312942	39.650342141483556
10	17.261109147183692	26.682801085761344	29.58853047794556	26.467559289109406
11	37.14204833070934	21.75404859287604	20.896080189540946	20.207822886873675
12	25.02410749957642	24.183848707398752	27.64389370806463	23.148150084960196
13	29.80939923385967	19.672054564140893	25.017256983325918	25.501289218673524
14	24.0102858593141	19.53385924716323	24.634395991638556	31.821458901884114
15	25.71165010228685	27.089350035769023	21.923689968358712	25.275309893585423
16	25.714130325646778	26.536544618851593	23.09523411091402	24.654090944587615
17	24.145971069626178	26.380732979036424	24.589981048694675	24.883314902642724
18	24.95692717677254	25.12468632316715	25.826053427732138	24.092333072328177
19	26.103784543599858	24.8810139715326	25.212836127686337	23.802365357181205
20	26.55819226687603	23.961890790227823	24.72678033794591	24.75313660495024
21	27.46300730301589	23.465522987696293	24.76673439306318	24.304735316224633
22	26.96084651952793	23.905698081581257	24.7129762753085	24.42047912358231
23	25.129375081055528	24.13007372741078	25.39907209723595	25.341479094297735
24	24.905800355323013	24.83495887568436	24.747104284793906	25.51213648419872
25	25.373309161902107	24.493027471761263	24.953632687212384	25.18003067912425
26	24.746391186148067	24.978228235033264	25.617889313671693	24.657491265146977
27	25.55637366667643	25.087958566390416	24.949553687845054	24.406114079088105
28	24.904178494007365	24.744644489487698	25.566858205089048	24.784318811415883
29	24.88348872817941	24.925045112383522	25.45119356071276	24.740272598724303
30	25.012167180547014	24.83938624418311	25.263320099345556	24.88512647592432
31	25.180048738142304	24.489696310473818	24.501898277430893	25.828356673952985
32	25.280646609788953	24.632823590770528	24.752821805788365	25.333707993652155
33	24.87831169637086	24.25754620878443	25.523062731270528	25.34107936357418
34	25.499499715866502	24.20120137918051	25.705609806266278	24.59368909868671
35	25.905331129083205	24.430012850378503	25.342787158546336	24.321868861991955
36	24.95527116897388	24.513281987563783	24.85737673598764	25.6740701074747
37	25.61502300278767	24.279062373184843	24.82523041137268	25.28068421265481
38	25.056163636642538	24.073177976324118	25.551979428281374	25.318678958751974
39	25.85590064363812	23.95441053527738	25.358198454738595	24.831490366345903
40	25.454381917178974	24.839056729147448	25.065384837969017	24.64117651570456
41	24.98126110824072	25.340838651692287	24.99255673787794	24.68534350218905
42	25.94179311999085	24.76114372098407	24.783038589356863	24.514024569668216
43	25.558611985621994	24.284952253448942	24.789019164862445	25.367416596066615
44	24.872274925303405	25.177057947822206	24.645936400687525	25.304730726186865
45	25.111458195332492	24.994596177580064	24.955479475933814	24.93846615115363
46	25.299983335227587	24.525454916909723	24.949395570796344	25.225166177066345
47	25.251065936402906	24.528774181852413	24.869594926735395	25.35056495500929
48	25.826971832753664	25.210647715577473	24.122266321188608	24.840114130480256
49	25.266184060612566	25.279501979558738	24.369002578516454	25.08531138131224
50	25.07816313154555	25.678924542247138	24.61769094534856	24.625221380858747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	237.0
1	192.0
2	147.0
3	289.0
4	431.0
5	441.5
6	452.0
7	328.0
8	204.0
9	318.0
10	432.0
11	719.0
12	1006.0
13	1937.0
14	2868.0
15	3322.0
16	3776.0
17	3519.5
18	3263.0
19	2824.5
20	2386.0
21	2830.5
22	3275.0
23	4103.0
24	4931.0
25	6370.0
26	7809.0
27	9718.5
28	11628.0
29	13736.5
30	15845.0
31	18179.0
32	20513.0
33	23426.0
34	26339.0
35	30276.0
36	34213.0
37	39854.5
38	45496.0
39	52621.0
40	59746.0
41	68557.5
42	77369.0
43	79339.5
44	81310.0
45	87224.5
46	93139.0
47	100709.0
48	108279.0
49	111446.0
50	114613.0
51	111816.0
52	109019.0
53	106786.5
54	104554.0
55	103068.5
56	101583.0
57	98278.0
58	94973.0
59	88400.0
60	81827.0
61	72657.0
62	63487.0
63	55117.0
64	46747.0
65	40191.5
66	33636.0
67	30560.0
68	27484.0
69	23327.0
70	19170.0
71	16275.0
72	13380.0
73	10993.5
74	8607.0
75	6434.0
76	4261.0
77	3264.5
78	2268.0
79	1912.5
80	1557.0
81	1214.0
82	871.0
83	719.5
84	568.0
85	428.5
86	289.0
87	200.0
88	111.0
89	74.5
90	38.0
91	28.0
92	18.0
93	17.5
94	17.0
95	13.0
96	9.0
97	10.5
98	12.0
99	10.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03186440961593974
2	0.005438564442107875
3	4.1835111093137505E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	6.972518515522918E-5
11	0.0
12	6.972518515522918E-5
13	0.0
14	0.0
15	0.0
16	6.972518515522918E-4
17	0.0
18	2.0917555546568753E-4
19	0.0
20	5.578014812418334E-4
21	0.0
22	0.0
23	0.0
24	9.761525921732085E-4
25	1.3945037031045835E-4
26	3.486259257761459E-4
27	2.789007406209167E-4
28	0.0017431296288807293
29	4.1835111093137505E-4
30	0.0011156029624836668
31	4.8807629608660423E-4
32	9.761525921732085E-4
33	3.486259257761459E-4
34	0.001185328147638896
35	2.0917555546568753E-4
36	2.789007406209167E-4
37	0.001952305184346417
38	0.0
39	7.66977036707521E-4
40	6.972518515522918E-5
41	0.0013247785179493544
42	0.005299114071797418
43	0.004671587405400354
44	0.004671587405400354
45	0.0022312059249673336
46	0.0027192822210539377
47	0.001185328147638896
48	0.0037651599983823756
49	0.003067908146830084
50	0.001533954073415042
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1434202.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.632586078521484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77177171199111	44.610491420369804
2	12.812565026265741	14.51217383473297
3	3.607636392340767	6.129293355877357
4	1.524402157171167	3.4532334553711985
5	0.8376535378073446	2.3719243041926252
6	0.5189569353920598	1.7633923988781923
7	0.3384347581368064	1.3416504900502402
8	0.2361988684596879	1.0701242199754117
9	0.17934594741372115	0.9141142324267395
>10	1.032401182816286	10.590569344059855
>50	0.07395196560363312	2.910332292437417
>100	0.05888764426624206	6.662811748370501
>500	0.005690763927462403	2.1833950818259074
>1k	0.002103108407975236	1.486493821431789
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCA	1813	0.1264117606864305	No Hit
GCGTTAGCATCCGCAAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT	1787	0.12459890587239456	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1713	0.1194392421709076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.972518515522918E-5
2	0.0	0.0	0.0	0.0	6.972518515522918E-5
3	0.0	0.0	0.0	0.0	6.972518515522918E-5
4	0.0	0.0	0.0	0.0	6.972518515522918E-5
5	0.0	0.0	0.0	0.0	6.972518515522918E-5
6	0.0	0.0	0.0	0.0	1.3945037031045835E-4
7	0.0	0.0	0.0	0.0	1.3945037031045835E-4
8	0.0	0.0	0.0	0.0	1.3945037031045835E-4
9	0.0	0.0	0.0	0.0	1.3945037031045835E-4
10	0.0	0.0	0.0	0.0	1.3945037031045835E-4
11	0.0	0.0	0.0	0.0	1.3945037031045835E-4
12	0.0	0.0	0.0	0.0	1.3945037031045835E-4
13	0.0	0.0	0.0	0.0	1.3945037031045835E-4
14	0.0	0.0	0.0	0.0	1.3945037031045835E-4
15	0.0	0.0	0.0	0.0	1.3945037031045835E-4
16	0.0	0.0	0.0	0.0	1.3945037031045835E-4
17	0.0	0.0	0.0	0.0	2.789007406209167E-4
18	0.0	0.0	0.0	0.0	2.789007406209167E-4
19	0.0	0.0	0.0	0.0	2.789007406209167E-4
20	0.0	6.972518515522918E-5	0.0	0.0	3.486259257761459E-4
21	0.0	6.972518515522918E-5	0.0	0.0	3.486259257761459E-4
22	0.0	6.972518515522918E-5	0.0	0.0	3.486259257761459E-4
23	0.0	6.972518515522918E-5	0.0	0.0	4.1835111093137505E-4
24	0.0	6.972518515522918E-5	0.0	6.972518515522918E-5	4.1835111093137505E-4
25	0.0	6.972518515522918E-5	0.0	6.972518515522918E-5	6.275266663970626E-4
26	0.0	6.972518515522918E-5	0.0	6.972518515522918E-5	6.275266663970626E-4
27	0.0	6.972518515522918E-5	0.0	6.972518515522918E-5	6.275266663970626E-4
28	0.0	6.972518515522918E-5	0.0	2.789007406209167E-4	6.275266663970626E-4
29	0.0	1.3945037031045835E-4	0.0	6.275266663970626E-4	6.275266663970626E-4
30	0.0	1.3945037031045835E-4	0.0	0.0011853281476388961	6.275266663970626E-4
31	0.0	1.3945037031045835E-4	0.0	0.0021614807398121047	6.972518515522918E-4
32	0.0	1.3945037031045835E-4	0.0	0.003486259257761459	6.972518515522918E-4
33	0.0	1.3945037031045835E-4	0.0	0.004950488146021272	6.972518515522918E-4
34	0.0	1.3945037031045835E-4	0.0	0.007460594811609522	7.66977036707521E-4
35	0.0	1.3945037031045835E-4	0.0	0.010110151847508232	7.66977036707521E-4
36	0.0	1.3945037031045835E-4	0.0	0.014851464438063816	7.66977036707521E-4
37	0.0	2.0917555546568753E-4	0.0	0.021893708138741964	7.66977036707521E-4
38	0.0	2.0917555546568753E-4	0.0	0.030260730357369463	7.66977036707521E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1375	0.0	33.12908	1
GTATCAA	3205	0.0	26.503366	1
CGTCGTA	340	0.0	21.998362	10
ACCGTCG	325	0.0	20.983053	8
TACCGTC	365	0.0	20.491625	7
CGCAATA	235	0.0	19.66085	36
ATACCGT	395	0.0	18.935299	6
GTATTAG	205	0.0	18.248905	1
GTCTAAT	85	1.4280656E-4	18.122616	1
CTAGCGG	255	0.0	18.118824	29
GTCGTAG	400	0.0	17.598688	11
TATCAAC	4865	0.0	17.590258	2
ATCAACG	4910	0.0	17.428436	3
TCAACGC	4985	0.0	17.166224	4
CAACGCA	5085	0.0	16.871899	5
CCGTCGT	425	0.0	16.563473	9
GTATAAT	120	6.3915268E-6	16.504524	1
TAGGGCG	80	0.00198773	16.501074	31
AACGCAG	5215	0.0	16.409134	6
CGCGCAT	190	6.166374E-10	16.21158	40
>>END_MODULE