FastQCFastQC Report
Thu 2 Feb 2017
SRR4062148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249736
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21730.17387672276384772No Hit
TATCAACGCAGAGTACTTTTTTTTT14270.1141841156852327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT250.00603252918.999415
GGTATCA5150.014.5706041
ATTAGCC400.005279440414.2495493
CGGTCCA1850.013.35306910
TCGCGTA1153.765308E-1013.2185629
CGCGTAA1207.385097E-1012.66829710
GTCCTAA905.4120574E-712.6647431
GTATCAA13700.012.4798571
CGACTCA550.003064908812.0924646
AGAACCG951.0386084E-612.0005835
GTTTAAG658.0317E-411.6905341
GGGTAGG3250.011.6905321
CGTCGTA1251.8262654E-811.40146710
AAGGCGT752.0707159E-411.4014666
GTATTAG752.0761171E-411.3982691
GCGTAAC1354.7148205E-911.26025711
GTGTATT855.3289154E-511.1747731
GTCCTAG1451.2187229E-911.136241
ACACTAG600.005868936411.084766
CTAGACC600.00587243811.0838724
Produced by FastQC (version 0.11.5)