FastQCFastQC Report
Thu 2 Feb 2017
SRR4062148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249736
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.033.498181
GTATCAA14150.025.1958941
TATAACG450.001398713824.4430242
ATACCGT2900.020.4815676
ACCGTCG2700.020.3691848
CGTCGTA2700.019.555210
TACCGTC2900.018.2058377
TCTATAC1002.4843652E-517.5989743
CCGTCGT2950.017.1515459
GGCGTTA2600.016.92615542
CGGCGTT2800.016.5016841
TACCGTT800.00198917516.4990397
TATCAAC21900.016.2730262
TAGAGTA953.347912E-416.2095814
CGAGCCG2353.6379788E-1215.91460515
TCAACGC22250.015.81930354
GTCGTAG3350.015.76090811
ATCAACG22450.015.6783753
GTGTTAG2252.5465852E-1115.6497951
TAGGACG850.00298117115.5285094
Produced by FastQC (version 0.11.5)