##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1249736
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296545830479396	32.0	32.0	32.0	32.0	32.0
2	31.470106486489946	32.0	32.0	32.0	32.0	32.0
3	31.58197251259466	32.0	32.0	32.0	32.0	32.0
4	31.65487590979215	32.0	32.0	32.0	32.0	32.0
5	31.63350099541023	32.0	32.0	32.0	32.0	32.0
6	35.275390162402296	36.0	36.0	36.0	36.0	36.0
7	35.277796270572345	36.0	36.0	36.0	36.0	36.0
8	35.22818979368443	36.0	36.0	36.0	36.0	36.0
9	35.31407113182304	36.0	36.0	36.0	36.0	36.0
10	35.200487943053574	36.0	36.0	36.0	36.0	36.0
11	35.31838084203384	36.0	36.0	36.0	36.0	36.0
12	35.24017952591587	36.0	36.0	36.0	36.0	36.0
13	35.27429233054021	36.0	36.0	36.0	36.0	36.0
14	35.22839143627134	36.0	36.0	36.0	36.0	36.0
15	35.21195116408585	36.0	36.0	36.0	36.0	36.0
16	35.2270239474577	36.0	36.0	36.0	36.0	36.0
17	35.20459681084645	36.0	36.0	36.0	36.0	36.0
18	35.20263079562404	36.0	36.0	36.0	36.0	36.0
19	35.193167196911986	36.0	36.0	36.0	36.0	36.0
20	35.19806263082763	36.0	36.0	36.0	36.0	36.0
21	35.18970646600562	36.0	36.0	36.0	36.0	36.0
22	35.16901009493205	36.0	36.0	36.0	36.0	36.0
23	35.14380077072278	36.0	36.0	36.0	36.0	36.0
24	35.13120050954762	36.0	36.0	36.0	36.0	36.0
25	35.11415450943239	36.0	36.0	36.0	36.0	36.0
26	35.073481119212374	36.0	36.0	36.0	36.0	36.0
27	35.05635990321156	36.0	36.0	36.0	36.0	36.0
28	35.04468623773341	36.0	36.0	36.0	36.0	36.0
29	35.027497007367955	36.0	36.0	36.0	36.0	36.0
30	35.01044380573177	36.0	36.0	36.0	36.0	36.0
31	35.00343432532951	36.0	36.0	36.0	36.0	36.0
32	34.98401342363507	36.0	36.0	36.0	36.0	36.0
33	34.95894572933803	36.0	36.0	36.0	36.0	36.0
34	34.95182582561437	36.0	36.0	36.0	36.0	36.0
35	34.941287599941106	36.0	36.0	36.0	36.0	36.0
36	34.92006951868235	36.0	36.0	36.0	36.0	36.0
37	34.91183657988567	36.0	36.0	36.0	36.0	36.0
38	34.899568388843726	36.0	36.0	36.0	32.0	36.0
39	34.88798754296908	36.0	36.0	36.0	32.0	36.0
40	34.8702645998835	36.0	36.0	36.0	32.0	36.0
41	34.850004320912575	36.0	36.0	36.0	32.0	36.0
42	34.81941145970029	36.0	36.0	36.0	32.0	36.0
43	34.81432238488769	36.0	36.0	36.0	32.0	36.0
44	34.77437314760878	36.0	36.0	36.0	32.0	36.0
45	34.7649887656273	36.0	36.0	36.0	32.0	36.0
46	34.740753247085784	36.0	36.0	36.0	32.0	36.0
47	34.72243497826741	36.0	36.0	36.0	32.0	36.0
48	34.694431463925184	36.0	36.0	36.0	32.0	36.0
49	34.67382311144114	36.0	36.0	36.0	32.0	36.0
50	34.263434037268674	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	31.0
22	78.0
23	205.0
24	602.0
25	1310.0
26	2925.0
27	5474.0
28	9413.0
29	14854.0
30	22728.0
31	33359.0
32	50380.0
33	86299.0
34	197248.0
35	824819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21345247833667	17.427197909946486	12.111616797908665	26.247732813808184
2	16.49296942267005	19.0674602539522	36.9559024521399	27.48366787123785
3	19.245680084177845	22.118929212993155	27.822138293318183	30.813252409510817
4	13.126372289827613	15.165362924649687	33.849549024754026	37.858715760768675
5	15.08007697631898	36.08581019176065	32.74110111343605	16.09301171848432
6	36.5263620309298	33.69447714031717	15.583008530622141	14.196152298130881
7	31.81687972499792	29.47254460141982	19.117157543673223	19.593418129909036
8	28.7199856609716	33.23165852628075	18.038929821978403	20.00942599076925
9	26.886638458042338	14.617007111902034	18.17159784146412	40.32475658859151
10	16.9053304057817	26.662911206846886	30.155568856142416	26.276189531228994
11	37.632267934987865	21.29009646837412	21.030201578573397	20.047434018064617
12	24.774512377014023	23.165852628075047	27.807392921385	24.252242073525927
13	29.89407362835031	19.06938745463042	24.565348201540164	26.47119071547911
14	23.726771094055067	18.84614030483238	24.546384196342267	32.88070440477029
15	25.380400340551923	26.730365453183712	21.59320048394221	26.296033722322154
16	26.28575772366831	25.69571027341906	22.79292327142663	25.225608731486
17	24.6943354436457	25.94083870513452	24.264164591561737	25.10066125965804
18	25.189320590334567	24.92542401090794	25.140670159682237	24.74458523907526
19	26.080409046717907	24.731643108338968	24.769571149083607	24.41837669585952
20	26.19551918856008	24.03553090309979	24.696474195605607	25.072475712734523
21	26.670352778506825	23.933694796340987	24.451884237951056	24.944068187201136
22	26.341723371976162	23.720049674491253	24.7621097575808	25.176117195951786
23	25.12124570408927	24.025893489419754	25.096680496265204	25.75618031022577
24	25.065334739517237	24.379339979099285	24.964832066114116	25.590493215269365
25	25.410327974317692	24.022910512013777	24.971113806800176	25.595647706868352
26	25.05025093460337	24.759547677574638	25.099301608029904	25.090899779792082
27	25.455217598653153	24.45772373596899	24.78099304490883	25.306065620469028
28	25.480110617486268	24.180371157514692	25.063374601508187	25.276143623490853
29	24.94186744336777	24.653325118225535	25.136069390988453	25.268738047418243
30	25.231792477816978	24.649340610714265	25.063114289075045	25.05575262239371
31	25.413949081842414	24.249378466245936	24.62369902002911	25.712973431882542
32	25.460741731774377	24.421472261075245	24.495168533212134	25.62261747393825
33	25.162954676755213	24.369025728675062	24.828003399123332	25.640016195446393
34	25.6188956619291	24.342295831246208	24.830562859378777	25.20824564744592
35	25.692168954759065	24.224413093697763	25.163975287481865	24.919442664061307
36	25.05245116160719	24.73910448143365	24.806158750582124	25.40228560637704
37	25.796524156384955	24.20727672237821	24.73675913871287	25.259439982523958
38	25.171156148178497	24.425558677992793	24.889256610996245	25.51402856283247
39	25.725783156053485	24.257533093439402	24.48678462918464	25.529899121322476
40	25.77064275975086	24.44484275078897	24.730183014652695	25.05433147480748
41	25.071256075370602	24.730139119385335	25.02756621893899	25.171038586305073
42	25.875331787857426	24.557862922101773	24.652207904653153	24.91459738538765
43	25.64526880231869	24.111187486646507	24.71574563905918	25.527798071975628
44	25.109628415685346	24.523236422880814	24.664232969185612	25.702902192248228
45	25.26860253066914	24.59172495356919	24.790331189100822	25.349341326660845
46	25.406315890550168	24.511864800092823	24.602926290604582	25.478893018752423
47	25.274282097466866	24.57636408882971	24.937005738099106	25.212348075604318
48	25.696736134894905	24.85404310209621	24.26349381123548	25.185726951773407
49	25.30733646531257	24.671898368314036	24.4416010703463	25.57916409602709
50	25.217849146767513	25.02692607452241	24.604510777631432	25.150714001078644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	37.5
2	32.0
3	119.5
4	207.0
5	223.0
6	239.0
7	179.5
8	120.0
9	177.0
10	234.0
11	396.5
12	559.0
13	1113.5
14	1668.0
15	2047.5
16	2427.0
17	2315.0
18	2203.0
19	2028.5
20	1854.0
21	2155.0
22	2456.0
23	3050.0
24	3644.0
25	4573.5
26	5503.0
27	6886.0
28	8269.0
29	9762.5
30	11256.0
31	13252.5
32	15249.0
33	18009.5
34	20770.0
35	24192.0
36	27614.0
37	32147.5
38	36681.0
39	42110.5
40	47540.0
41	53078.0
42	58616.0
43	62391.5
44	66167.0
45	73349.5
46	80532.0
47	85366.0
48	90200.0
49	95045.0
50	99890.0
51	101380.0
52	102870.0
53	103186.5
54	103503.0
55	102401.5
56	101300.0
57	96807.5
58	92315.0
59	86000.0
60	79685.0
61	69319.0
62	58953.0
63	50969.5
64	42986.0
65	36091.0
66	29196.0
67	24595.0
68	19994.0
69	16906.5
70	13819.0
71	10779.5
72	7740.0
73	6622.0
74	5504.0
75	4189.0
76	2874.0
77	2465.0
78	2056.0
79	1693.5
80	1331.0
81	1038.5
82	746.0
83	603.0
84	460.0
85	351.5
86	243.0
87	174.5
88	106.0
89	70.0
90	34.0
91	24.0
92	14.0
93	13.0
94	12.0
95	10.0
96	8.0
97	9.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031206590831983714
2	0.004160878777597829
3	8.001689956918901E-5
4	0.0
5	8.001689956918901E-5
6	2.4005069870756702E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.8010139741513403E-4
17	0.0
18	3.2006759827675604E-4
19	8.001689956918901E-5
20	5.601182969843231E-4
21	0.0
22	0.0
23	8.001689956918901E-5
24	0.0014403041922454022
25	3.2006759827675604E-4
26	6.401351965535121E-4
27	3.2006759827675604E-4
28	0.0019204055896605361
29	4.8010139741513403E-4
30	0.0015203210918145913
31	4.00084497845945E-4
32	9.602027948302681E-4
33	1.6003379913837802E-4
34	8.0016899569189E-4
35	4.00084497845945E-4
36	1.6003379913837802E-4
37	0.0020004224892297253
38	0.0
39	0.0013602872926762133
40	0.0
41	0.0014403041922454022
42	0.005361132271135664
43	0.005041064672858908
44	0.004801013974151341
45	0.002160456288368103
46	0.002480523886644859
47	0.0015203210918145913
48	0.004160878777597829
49	0.0037607942797518836
50	0.0014403041922454022
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1249736.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.75737708143607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68423691610016	42.75711913933177
2	13.217733860708297	14.739723420671567
3	4.103125732329863	6.86338586010179
4	1.8058806138752463	4.027646654075894
5	0.9950461419873418	2.7740581476108193
6	0.6454526273795302	2.1593247319802464
7	0.4629818865374394	1.8070258940679855
8	0.3364388442357983	1.500715800231835
9	0.25697101337387573	1.2895226722517343
>10	1.3715560246176606	13.65406732402517
>50	0.0765770680914343	2.891602949318086
>100	0.040825561384803935	4.282705509585702
>500	0.002885190344058774	1.0779137632911848
>1k	2.8851903440587734E-4	0.1751881334563242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.001689956918901E-5
7	0.0	0.0	0.0	0.0	8.001689956918901E-5
8	0.0	0.0	0.0	0.0	8.001689956918901E-5
9	0.0	0.0	0.0	0.0	8.001689956918901E-5
10	0.0	0.0	0.0	0.0	8.001689956918901E-5
11	0.0	0.0	0.0	0.0	8.001689956918901E-5
12	0.0	0.0	0.0	0.0	2.4005069870756704E-4
13	0.0	0.0	0.0	0.0	4.0008449784594507E-4
14	0.0	0.0	0.0	1.6003379913837802E-4	4.0008449784594507E-4
15	0.0	0.0	0.0	1.6003379913837802E-4	5.601182969843231E-4
16	0.0	0.0	0.0	1.6003379913837802E-4	5.601182969843231E-4
17	0.0	0.0	0.0	1.6003379913837802E-4	5.601182969843231E-4
18	0.0	0.0	0.0	4.0008449784594507E-4	7.201520961227011E-4
19	0.0	0.0	0.0	4.801013974151341E-4	7.201520961227011E-4
20	0.0	0.0	0.0	5.601182969843231E-4	8.801858952610792E-4
21	0.0	0.0	0.0	7.201520961227011E-4	8.801858952610792E-4
22	0.0	0.0	0.0	8.801858952610792E-4	8.801858952610792E-4
23	0.0	0.0	0.0	0.0010402196943994572	8.801858952610792E-4
24	0.0	0.0	0.0	0.0016003379913837803	8.801858952610792E-4
25	0.0	0.0	0.0	0.0018403886900913472	8.801858952610792E-4
26	0.0	0.0	0.0	0.0022404731879372922	0.0011202365939686461
27	0.0	0.0	0.0	0.0028806083844908044	0.0011202365939686461
28	0.0	0.0	0.0	0.004320912576736207	0.0011202365939686461
29	0.0	0.0	0.0	0.005921250568119987	0.0011202365939686461
30	0.0	0.0	0.0	0.008401774454764846	0.0011202365939686461
31	0.0	0.0	0.0	0.013122771529346999	0.0011202365939686461
32	0.0	0.0	0.0	0.01944410659531293	0.0012002534935378352
33	0.0	0.0	0.0	0.02592547546041724	0.0012002534935378352
34	0.0	0.0	0.0	0.03232682742595236	0.0013602872926762133
35	0.0	0.0	0.0	0.03936831458804099	0.0013602872926762133
36	0.0	0.0	0.0	0.04873029183763611	0.0013602872926762133
37	0.0	0.0	0.0	0.06273324926224419	0.0013602872926762133
38	0.0	0.0	0.0	0.08177727135971118	0.0013602872926762133
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	565	0.0	33.49818	1
GTATCAA	1415	0.0	25.195894	1
TATAACG	45	0.0013987138	24.443024	2
ATACCGT	290	0.0	20.481567	6
ACCGTCG	270	0.0	20.369184	8
CGTCGTA	270	0.0	19.5552	10
TACCGTC	290	0.0	18.205837	7
TCTATAC	100	2.4843652E-5	17.598974	3
CCGTCGT	295	0.0	17.151545	9
GGCGTTA	260	0.0	16.926155	42
CGGCGTT	280	0.0	16.50168	41
TACCGTT	80	0.001989175	16.499039	7
TATCAAC	2190	0.0	16.273026	2
TAGAGTA	95	3.347912E-4	16.209581	4
CGAGCCG	235	3.6379788E-12	15.914605	15
TCAACGC	2225	0.0	15.8193035	4
GTCGTAG	335	0.0	15.760908	11
ATCAACG	2245	0.0	15.678375	3
GTGTTAG	225	2.5465852E-11	15.649795	1
TAGGACG	85	0.002981171	15.528509	4
>>END_MODULE