FastQCFastQC Report
Thu 2 Feb 2017
SRR4062147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638652
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53640.2032856170499179No Hit
TATCAACGCAGAGTACTTTTTTTTT36250.13738075350595683No Hit
GCGCAAGACGGACCAGAGCGAAAGC26750.1013775215526716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG2600.012.42509910
TCGCGTA2650.012.1902019
GGTATCA15850.012.044481
CGCGCGG802.8633583E-511.87693410
GGTCGGC2650.011.83233911
GCGGTCG2650.011.8316669
CGAACGA907.4713607E-611.61035716
CGTCGTA4350.011.57659710
AACCGCG1406.8030204E-1011.5371547
GTATCAA38200.011.5365481
GCGTTAT2150.011.4856641
CGACCAT3750.011.40185610
CCGTCGT4250.011.4014249
CGCGTAA2800.011.19825210
GTCGGCG2800.011.19803912
CGTCTTA1451.2187229E-911.13805415
CGCATCG4650.011.033215513
ACACCGT1553.1286618E-1011.0327996
CGGCGTC3100.011.0325914
TTAGGAC4400.011.010443
Produced by FastQC (version 0.11.5)