FastQCFastQC Report
Thu 2 Feb 2017
SRR4062147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638652
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26660.10103643830258784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.024.4067881
GTATCAA42500.023.4059731
TAGGACG8450.020.8279194
GTAGGAC11900.019.2264423
TGTAGGA13050.019.051172
GATATAC7800.018.3398571
AACGAAT5450.017.35772331
GGACGTG10250.016.7410746
CGCAATA6050.016.72720136
AACGCAG60450.016.4495756
CTGTAGG13450.016.3626981
ATCAACG60500.016.3268933
TCAACGC60900.016.2196564
TATGGCG12000.016.13295716
CAACGCA61900.015.9931665
ATACCGT6500.015.9073226
GGACCGT1805.2768883E-915.8885196
TATCAAC62500.015.8763342
TAGGACC17400.015.8042314
ACCGTCG6150.015.7394718
Produced by FastQC (version 0.11.5)