##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2638652
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266774853220507	32.0	32.0	32.0	32.0	32.0
2	31.43971543045464	32.0	32.0	32.0	32.0	32.0
3	31.578534797313175	32.0	32.0	32.0	32.0	32.0
4	31.655703745700457	32.0	32.0	32.0	32.0	32.0
5	31.631191229461102	32.0	32.0	32.0	32.0	32.0
6	35.27684097789326	36.0	36.0	36.0	36.0	36.0
7	35.27924940462024	36.0	36.0	36.0	36.0	36.0
8	35.23024028935987	36.0	36.0	36.0	36.0	36.0
9	35.316305446872114	36.0	36.0	36.0	36.0	36.0
10	35.19686301945084	36.0	36.0	36.0	36.0	36.0
11	35.31904699824001	36.0	36.0	36.0	36.0	36.0
12	35.241846215416054	36.0	36.0	36.0	36.0	36.0
13	35.27317395397347	36.0	36.0	36.0	36.0	36.0
14	35.236637874187274	36.0	36.0	36.0	36.0	36.0
15	35.21555021276015	36.0	36.0	36.0	36.0	36.0
16	35.228426105450815	36.0	36.0	36.0	36.0	36.0
17	35.209600583934524	36.0	36.0	36.0	36.0	36.0
18	35.20370780231725	36.0	36.0	36.0	36.0	36.0
19	35.19204957682938	36.0	36.0	36.0	36.0	36.0
20	35.187681058358585	36.0	36.0	36.0	36.0	36.0
21	35.17785748177479	36.0	36.0	36.0	36.0	36.0
22	35.16811121739433	36.0	36.0	36.0	36.0	36.0
23	35.13282198637789	36.0	36.0	36.0	36.0	36.0
24	35.12161020096625	36.0	36.0	36.0	36.0	36.0
25	35.10171254110053	36.0	36.0	36.0	36.0	36.0
26	35.0625224546473	36.0	36.0	36.0	36.0	36.0
27	35.049598431320234	36.0	36.0	36.0	36.0	36.0
28	35.02782102376516	36.0	36.0	36.0	36.0	36.0
29	35.00651544803938	36.0	36.0	36.0	36.0	36.0
30	34.984754715665424	36.0	36.0	36.0	36.0	36.0
31	34.98277264300105	36.0	36.0	36.0	36.0	36.0
32	34.95489552999031	36.0	36.0	36.0	36.0	36.0
33	34.933389094128366	36.0	36.0	36.0	36.0	36.0
34	34.920450290527135	36.0	36.0	36.0	36.0	36.0
35	34.90449896386488	36.0	36.0	36.0	36.0	36.0
36	34.88198633241519	36.0	36.0	36.0	32.0	36.0
37	34.86166572931936	36.0	36.0	36.0	32.0	36.0
38	34.843066080710905	36.0	36.0	36.0	32.0	36.0
39	34.81648167321799	36.0	36.0	36.0	32.0	36.0
40	34.806252207566594	36.0	36.0	36.0	32.0	36.0
41	34.77828717087361	36.0	36.0	36.0	32.0	36.0
42	34.7472069071632	36.0	36.0	36.0	32.0	36.0
43	34.74951983058016	36.0	36.0	36.0	32.0	36.0
44	34.69696875525837	36.0	36.0	36.0	32.0	36.0
45	34.672610105462944	36.0	36.0	36.0	32.0	36.0
46	34.636115334648146	36.0	36.0	36.0	32.0	36.0
47	34.603998177857484	36.0	36.0	36.0	32.0	36.0
48	34.57280042991649	36.0	36.0	36.0	32.0	36.0
49	34.557673008793884	36.0	36.0	36.0	32.0	36.0
50	34.14648691832041	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	9.0
20	35.0
21	65.0
22	202.0
23	500.0
24	1193.0
25	2734.0
26	5933.0
27	11538.0
28	20090.0
29	32541.0
30	49388.0
31	73741.0
32	112152.0
33	190145.0
34	433341.0
35	1705043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34488715781604	18.495192393355218	12.441698552467418	25.71822189636132
2	15.8326678701223	20.190766541608856	37.92067729057996	26.055888297688888
3	18.752254942682768	23.662724243627796	28.76564058563325	28.819380228056186
4	12.585213965312592	16.402466107694384	35.68462229956811	35.32769762742491
5	14.43161129243265	36.756836445275844	33.28578380172906	15.525768460562439
6	34.21310239679654	35.42224480955581	16.721296937407697	13.643355856239959
7	30.28527445074227	30.382407380738346	20.1729898448147	19.15932832370468
8	27.77819886820998	33.91750787902308	18.961196853544916	19.343096399222027
9	26.778104880825516	14.766213960764816	18.99197014233025	39.46371101607942
10	16.61830860477896	26.90678945672977	30.91186781119133	25.563034127299943
11	36.81694289356838	21.483469589775385	22.279671589887563	19.41991592676867
12	24.4771951738994	23.835011210269485	28.813576022908666	22.874217592922445
13	29.470805547681167	19.688803222251362	25.269190480593878	25.571200749473594
14	23.287913677135144	20.112580211410975	25.35741734794888	31.242088763505
15	24.882705260110086	27.756217947649027	22.28683433813932	25.074242454101565
16	25.237017116471133	26.30594384657481	24.203601265506165	24.25343777144789
17	23.54941083553269	26.529152006403265	25.379739351759913	24.54169780630413
18	24.201722774250932	25.42302866123637	26.79054562350231	23.584702941010388
19	25.45663467558435	25.162279830762067	25.576620183336036	23.80446531031754
20	25.516278241294298	24.89141206945231	25.37643373777071	24.215875951482673
21	26.40917650723798	24.55504725710221	24.96089858037308	24.074877655286738
22	25.61667851615143	24.742292655492275	25.475242661783366	24.165786166572932
23	24.24328786061974	24.820893395567133	25.934568105229488	25.001250638583638
24	24.555773261124145	25.265876610210604	25.892034881737874	24.286315246927384
25	24.623396291056523	24.869241797601727	25.87437738024269	24.63298453109906
26	23.97808422270959	26.000219051657915	26.194219992291508	23.827476733340987
27	24.716993717203675	25.165255961986393	25.66127018752885	24.456480133281083
28	24.17918966486926	25.277759670614703	25.964370661544784	24.57868000297126
29	24.236226148233126	25.21388851306411	25.83936200491086	24.710523333791905
30	24.124041877872	25.33323227059548	26.095144251267232	24.447581600265288
31	24.864920995315394	24.96986137939947	25.465116323137554	24.700101302147583
32	24.615870881529094	25.24494709567196	25.284475169131266	24.85470685366768
33	24.182526740009756	25.066047056656643	25.872292980233446	24.879133223100155
34	24.833246672134155	25.357799583723057	25.927527487770156	23.881426256372627
35	25.073598297309836	25.076819643999503	26.019234092611065	23.8303479660796
36	23.913190128247884	25.604364369523697	25.74981808810599	24.732627414122426
37	24.95984616095948	25.322235032035906	25.41535252379859	24.30256628320603
38	24.396851119435226	25.192029869797157	25.75296022362934	24.65815878713828
39	25.166431064605494	24.844635284219464	25.329583912863875	24.65934973831117
40	25.45564036747593	25.144458395186327	25.54913518243616	23.85076605490158
41	24.202367751045813	25.394846249292247	26.31646366929405	24.086322330367896
42	25.226016686614706	25.703215663920936	25.519286934124487	23.551480715339874
43	24.696442201791747	24.96390051778596	25.8176674845426	24.521989795879694
44	24.14172491840209	25.634705148437703	25.4910276409601	24.732542292200105
45	24.36271810425951	25.68440935666752	25.465656076031546	24.48721646304142
46	24.276163694108195	25.364476347126107	25.397903417747425	24.961456541018276
47	24.705716936454568	25.318121318145575	25.753652672461623	24.222509072938237
48	25.146406281030327	25.657407028411082	24.82759457095342	24.368592119605175
49	24.597630142255895	25.78032294191328	25.019035040012433	24.603011875818392
50	24.654609289905352	26.026432138788437	24.824206418085915	24.494752153220297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	713.0
1	408.0
2	103.0
3	271.5
4	440.0
5	474.0
6	508.0
7	408.0
8	308.0
9	429.5
10	551.0
11	945.0
12	1339.0
13	2605.5
14	3872.0
15	5123.0
16	6374.0
17	6718.0
18	7062.0
19	7197.5
20	7333.0
21	8680.5
22	10028.0
23	11960.5
24	13893.0
25	17774.5
26	21656.0
27	27546.0
28	33436.0
29	38470.0
30	43504.0
31	48194.5
32	52885.0
33	59963.5
34	67042.0
35	76324.0
36	85606.0
37	96229.5
38	106853.0
39	113630.0
40	120407.0
41	127953.5
42	135500.0
43	138053.5
44	140607.0
45	152381.0
46	164155.0
47	174382.5
48	184610.0
49	192532.5
50	200455.0
51	193908.0
52	187361.0
53	186478.0
54	185595.0
55	185842.5
56	186090.0
57	179492.5
58	172895.0
59	157729.5
60	142564.0
61	125229.5
62	107895.0
63	92376.5
64	76858.0
65	65210.5
66	53563.0
67	46192.0
68	38821.0
69	34332.0
70	29843.0
71	23051.0
72	16259.0
73	14428.0
74	12597.0
75	9273.5
76	5950.0
77	5332.5
78	4715.0
79	4096.5
80	3478.0
81	2803.0
82	2128.0
83	1770.5
84	1413.0
85	1103.0
86	793.0
87	536.5
88	280.0
89	191.0
90	102.0
91	71.0
92	40.0
93	50.5
94	61.0
95	59.0
96	57.0
97	48.5
98	40.0
99	27.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03232711248016032
2	0.005267841306849103
3	1.5159255559277993E-4
4	0.0
5	0.0
6	1.8949069449097493E-4
7	0.0
8	0.0
9	0.0
10	7.579627779638997E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.095553335566797E-4
17	0.0
18	3.7898138898194984E-5
19	0.0
20	2.6528697228736487E-4
21	3.7898138898194984E-5
22	0.0
23	0.0
24	8.337590557602897E-4
25	1.1369441669458496E-4
26	1.8949069449097493E-4
27	6.821665001675097E-4
28	0.0015159255559277995
29	4.1687952788014486E-4
30	0.0010232497502512645
31	4.1687952788014486E-4
32	7.958609168620947E-4
33	1.1369441669458496E-4
34	9.095553335566797E-4
35	1.5159255559277993E-4
36	4.5477766677833986E-4
37	0.0018949069449097494
38	0.0
39	8.337590557602897E-4
40	1.1369441669458496E-4
41	0.0011369441669458495
42	0.004926758056765348
43	0.004168795278801448
44	0.003941406445412279
45	0.002387582750586284
46	0.002728666000670039
47	0.0013643330003350195
48	0.003941406445412279
49	0.003676119473124914
50	0.0017433143893169694
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2638652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.4018194197887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.80670435397958	33.787714984318974
2	16.551759208114472	16.02270520541963
3	5.984694803828804	8.690103515324086
4	2.7020032543459394	5.231274943541341
5	1.451437134483401	3.512609904122057
6	0.8730574846607971	2.5354542429388105
7	0.5544996444566092	1.8787154161528083
8	0.38311176321931983	1.4834645104750692
9	0.2731500721566148	1.1898864420324051
>10	1.2608155339374043	10.560431231969366
>50	0.08086330489392239	2.6989135341791495
>100	0.06506597957207776	6.4607811127878865
>500	0.008820833026499657	2.9377203271853314
>1k	0.004016629324559356	3.0102246295532153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2666	0.10103643830258784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.789813889819499E-5	0.0
3	0.0	0.0	0.0	3.789813889819499E-5	0.0
4	0.0	0.0	0.0	3.789813889819499E-5	0.0
5	0.0	0.0	0.0	3.789813889819499E-5	0.0
6	0.0	0.0	0.0	3.789813889819499E-5	7.579627779638998E-5
7	0.0	0.0	0.0	3.789813889819499E-5	7.579627779638998E-5
8	0.0	0.0	0.0	7.579627779638998E-5	7.579627779638998E-5
9	0.0	0.0	0.0	7.579627779638998E-5	7.579627779638998E-5
10	0.0	0.0	0.0	1.1369441669458496E-4	7.579627779638998E-5
11	0.0	0.0	0.0	1.1369441669458496E-4	7.579627779638998E-5
12	0.0	0.0	0.0	1.1369441669458496E-4	3.4108325008375487E-4
13	0.0	0.0	0.0	1.1369441669458496E-4	4.1687952788014486E-4
14	0.0	0.0	0.0	1.1369441669458496E-4	4.1687952788014486E-4
15	0.0	0.0	0.0	1.1369441669458496E-4	4.5477766677833986E-4
16	0.0	0.0	0.0	1.1369441669458496E-4	4.926758056765349E-4
17	0.0	0.0	0.0	1.1369441669458496E-4	5.684720834729248E-4
18	0.0	0.0	0.0	1.1369441669458496E-4	6.063702223711198E-4
19	0.0	0.0	0.0	1.5159255559277996E-4	6.063702223711198E-4
20	0.0	0.0	0.0	1.8949069449097493E-4	7.579627779638997E-4
21	0.0	0.0	0.0	2.2738883338916993E-4	7.958609168620947E-4
22	0.0	0.0	0.0	3.4108325008375487E-4	7.958609168620947E-4
23	0.0	0.0	0.0	4.5477766677833986E-4	7.958609168620947E-4
24	0.0	0.0	0.0	6.442683612693147E-4	7.958609168620947E-4
25	0.0	0.0	0.0	7.579627779638997E-4	7.958609168620947E-4
26	0.0	0.0	0.0	7.958609168620947E-4	9.095553335566797E-4
27	0.0	0.0	0.0	0.0011369441669458497	0.0010232497502512647
28	0.0	0.0	0.0	0.0018191106671133594	0.0010232497502512647
29	0.0	0.0	0.0	0.002804462278466429	0.0010232497502512647
30	0.0	0.0	0.0	0.004434082251088814	0.0010232497502512647
31	0.0	0.0	0.0	0.010308293780309036	0.0010611478891494597
32	0.0	0.0	0.0	0.016371996004020235	0.0011369441669458497
33	0.0	0.0	0.0	0.021943022422054898	0.0011748423058440447
34	0.0	0.0	0.0	0.029067872534915554	0.0011748423058440447
35	0.0	0.0	0.0	0.03668539845345275	0.0011748423058440447
36	0.0	0.0	0.0	0.04748636803943832	0.0011748423058440447
37	0.0	0.0	0.0	0.06556378029387733	0.0011748423058440447
38	0.0	3.789813889819499E-5	0.0	0.08780998782711778	0.0013643330003350195
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2110	0.0	24.406788	1
GTATCAA	4250	0.0	23.405973	1
TAGGACG	845	0.0	20.827919	4
GTAGGAC	1190	0.0	19.226442	3
TGTAGGA	1305	0.0	19.05117	2
GATATAC	780	0.0	18.339857	1
AACGAAT	545	0.0	17.357723	31
GGACGTG	1025	0.0	16.741074	6
CGCAATA	605	0.0	16.727201	36
AACGCAG	6045	0.0	16.449575	6
CTGTAGG	1345	0.0	16.362698	1
ATCAACG	6050	0.0	16.326893	3
TCAACGC	6090	0.0	16.219656	4
TATGGCG	1200	0.0	16.132957	16
CAACGCA	6190	0.0	15.993166	5
ATACCGT	650	0.0	15.907322	6
GGACCGT	180	5.2768883E-9	15.888519	6
TATCAAC	6250	0.0	15.876334	2
TAGGACC	1740	0.0	15.804231	4
ACCGTCG	615	0.0	15.739471	8
>>END_MODULE