FastQCFastQC Report
Thu 2 Feb 2017
SRR4062146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52162
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT4500.8626969824776657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4160.7975154326904644No Hit
TATCAACGCAGAGTACTTTTTTTTT3760.7208312564702274No Hit
GATATACACTGTTCTACAAATCCCG2510.48119320578198693No Hit
GTCCTACAGTGTGCATTTCTCATTT2470.47352478815996313No Hit
GTCCTACAGTGGACATTTCTAAATT2360.452436639699398No Hit
GTGTATATCAATGAGTTACAATGAG2210.42368007361680915No Hit
GGTATCAACGCAGAGTACTTTTTTT2170.41601165599478546No Hit
GTCCTAAAGTGTGTATTTCTCATTT2140.41026034277826773No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2010.38533798550669074No Hit
CTGTAGGACGTGGAATATGGCAAGA1910.3661669414516315No Hit
TTGTAGAACAGTGTATATCAATGAG1900.36424983704612557No Hit
GTACATGGGGTGGTATCAACGCAAA1740.33357616655803074No Hit
CTTTAGGACGTGAAATATGGCGAGG1730.33165906215252483No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1670.32015643571948926No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1550.2971511828534182No Hit
GTTCTACAAATCCCGTTTCCAACGA1480.28373145201487676No Hit
CTGTAGAACAGATTAGATGAGTGAG1460.2798972432038649No Hit
GTTCTACAGTGTGGTTTTTATCATT1450.2779801387983589No Hit
CTGTAGGACCTGGAATATGGCGAGA1350.2588090947432997No Hit
GTATCAACGCAGAGTACATGGGGTG1200.23005252866071085No Hit
CCATAGGGTCTTCTCGTCTTATTAT1120.21471569341666347No Hit
GTGTATATCAATGAGTTACAATGAA990.18979333614508645No Hit
GGTATCAACGCAGAGTACATGGGGT830.15911966565699168No Hit
TTGATATACACTGTTCTACAAATCC810.15528545684597983No Hit
GTACATGGGTGGTATCAACGCAAAA800.15336835244047392No Hit
GTCCTTCAGTGTGCATTTCTCATTT780.14953414362946205No Hit
GATTAGATGAGTGAGTTACACTGAA770.14761703922395614No Hit
TATCAACGCAGAGTACATGGGGTGG760.1456999348184502No Hit
GCAGAGTACTTTTTTTTTTTTTTTT750.1437828304129443No Hit
CTGAAGGACCTGGAATATGGCGAGA720.1380315171964265No Hit
ACGCAGAGTACATGGGGTGGTATCA710.1361144127909206No Hit
ATTTAGAAATGTCCACTGTAGGACG640.12269468195237913No Hit
GAGTTACACTGAAAAACACATTCGT610.11694336873586135No Hit
GTATATCAATGAGTTACAATGAGAA610.11694336873586135No Hit
GTATTAGAGGCACTGCCTGCCCAGT600.11502626433035543No Hit
CTGTAGGACATGGAATATGGCAAGA590.1131091599248495No Hit
GTACATGGGAGTGGTATCAACGCAA560.10735784670833173No Hit
ATCCTGACCGTGCAAAGGTAGCATA560.10735784670833173No Hit
GTAGAACAGATTAGATGAGTGAGTT550.10544074230282581No Hit
ATCAACGCAGAGTACTTTTTTTTTT550.10544074230282581No Hit
GATATACACTGTTCTACAATGCCGG530.10160653349181396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGG451.5981459E-619.0096447
TAGGACC401.2514933E-518.991334
TTGTAGA359.885783E-518.9548071
CGTGGAA504.3721284E-617.1086779
ACGTGGA551.0817554E-515.5533448
CTGTAGG1055.456968E-1215.3443681
TCCTAAA400.00527152214.2161052
GTCCTAA400.00527152214.2161051
TGTAGGA1203.6379788E-1214.2161052
GGACGTG1152.1827873E-1114.0506056
GAACAGT551.8951263E-413.8251946
AACAGTG551.8951263E-413.8251947
TACACTG1255.456968E-1213.6869445
CCTACAG1400.013.5391473
ACTGTTC1009.1840775E-913.3067498
AGGACCT500.001460150313.3067495
GTAGGAC1301.2732926E-1113.122563
GGCAAGA1102.4738256E-912.9611219
TGGCAAG1206.5847416E-1012.67309518
GGACCTG603.9578334E-412.6730956
Produced by FastQC (version 0.11.5)