##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062146_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	52162
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97551857674169	32.0	32.0	32.0	32.0	32.0
2	30.767167669951306	32.0	32.0	32.0	32.0	32.0
3	30.86400061347341	32.0	32.0	32.0	32.0	32.0
4	30.755990951267204	32.0	32.0	32.0	32.0	32.0
5	30.88643073501783	32.0	32.0	32.0	32.0	32.0
6	34.422069705916186	36.0	36.0	36.0	32.0	36.0
7	34.27753920478509	36.0	36.0	36.0	32.0	36.0
8	34.19353168973582	36.0	36.0	36.0	32.0	36.0
9	34.162666308807175	36.0	36.0	36.0	32.0	36.0
10	34.02781718492389	36.0	36.0	36.0	32.0	36.0
11	34.356984011349255	36.0	36.0	36.0	32.0	36.0
12	34.160615007093284	36.0	36.0	36.0	32.0	36.0
13	34.22775200337411	36.0	36.0	36.0	32.0	36.0
14	34.16849430619992	36.0	36.0	36.0	32.0	36.0
15	34.21943177025421	36.0	36.0	36.0	32.0	36.0
16	34.20886852497987	36.0	36.0	36.0	32.0	36.0
17	34.14079214754035	36.0	36.0	36.0	32.0	36.0
18	34.187742034431196	36.0	36.0	36.0	32.0	36.0
19	34.04823434684253	36.0	36.0	36.0	32.0	36.0
20	33.90123078102833	36.0	36.0	36.0	32.0	36.0
21	33.86049231241133	36.0	36.0	36.0	32.0	36.0
22	33.85284306583336	36.0	36.0	36.0	32.0	36.0
23	33.85775085311146	36.0	36.0	36.0	32.0	36.0
24	33.84990989609294	36.0	36.0	36.0	32.0	36.0
25	33.52104980637245	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	5.0
6	10.0
7	1.0
8	9.0
9	9.0
10	6.0
11	3.0
12	0.0
13	1.0
14	51.0
15	35.0
16	52.0
17	54.0
18	75.0
19	104.0
20	142.0
21	193.0
22	240.0
23	327.0
24	417.0
25	479.0
26	657.0
27	751.0
28	922.0
29	1181.0
30	1526.0
31	2093.0
32	2984.0
33	4467.0
34	10197.0
35	25167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.828840918676434	21.322035198036886	12.340401058241632	22.50872282504505
2	12.626049614662014	22.276753191978834	46.07377017752387	19.02342701583528
3	19.924848066563143	27.541649891681523	29.53355955599011	22.999942485765228
4	10.006902634505503	17.987115082256395	39.63646124937685	32.36952103386126
5	10.726889144610665	41.361718450326045	35.32604526275412	12.585347142309168
6	28.12931696085956	40.76170376055257	18.591711435149655	12.517267843438221
7	25.65219895187453	34.04296162632215	20.373178738026223	19.931660683777093
8	22.947578555387064	41.77591799911703	19.534714091022515	15.741789354473386
9	26.15614981474727	15.177285903515003	21.157205659326948	37.50935862241078
10	16.155293575515532	29.971967282362428	32.77139894781306	21.101340194308975
11	33.529197150373484	23.734086064865487	25.464216448720162	17.272500336040864
12	23.39445137755592	27.46856100604781	32.55639819525775	16.580589421138523
13	30.01247960065278	23.07958145339349	27.551118364212346	19.356820581741385
14	19.294709360362436	25.860016893188977	28.234661752284417	26.610611994164167
15	21.964707463660975	35.263733942664025	24.04040016129342	18.731158432381577
16	18.969091956229605	29.282011902476484	32.086772893069686	19.662123248224226
17	16.434043043369748	31.737285695087063	31.034615163092518	20.79405609845067
18	17.07101727447217	29.59692898272553	35.83109404990403	17.50095969289827
19	22.986277708473274	26.21437482007485	28.630649649745703	22.16869782170617
20	22.566159396649333	29.643631618338485	30.154100059490684	17.636108925521505
21	22.09791014987814	26.73050720604886	28.112226294881882	23.05935634919112
22	21.021822159952404	31.595109686582347	29.24591674183828	18.13715141162697
23	20.877223277499567	30.522458220610527	30.271110343636675	18.32920815825323
24	21.99577816158127	29.31107273076185	31.0324313951257	17.660717712531184
25	21.088487593314014	30.22510506822238	31.533900094033662	17.152507244429945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	462.0
1	462.0
2	288.0
3	114.0
4	114.0
5	114.0
6	227.0
7	340.0
8	340.0
9	340.0
10	307.5
11	275.0
12	275.0
13	275.0
14	252.0
15	229.0
16	229.0
17	229.0
18	432.0
19	635.0
20	635.0
21	635.0
22	1064.5
23	1494.0
24	1494.0
25	1494.0
26	2544.0
27	3594.0
28	3594.0
29	3594.0
30	4855.5
31	6117.0
32	6117.0
33	6117.0
34	6291.5
35	6466.0
36	6466.0
37	6466.0
38	6454.5
39	6443.0
40	6443.0
41	6443.0
42	6091.5
43	5740.0
44	5740.0
45	5740.0
46	6170.0
47	6600.0
48	6600.0
49	6600.0
50	5984.0
51	5368.0
52	5368.0
53	5368.0
54	4345.0
55	3322.0
56	3322.0
57	3322.0
58	2695.5
59	2069.0
60	2069.0
61	2069.0
62	1721.5
63	1374.0
64	1374.0
65	1374.0
66	1038.0
67	702.0
68	702.0
69	702.0
70	551.0
71	400.0
72	400.0
73	400.0
74	318.0
75	236.0
76	236.0
77	236.0
78	174.5
79	113.0
80	113.0
81	113.0
82	73.0
83	33.0
84	33.0
85	33.0
86	21.0
87	9.0
88	9.0
89	9.0
90	7.5
91	6.0
92	6.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	10.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001917104405505924
4	0.015336835244047392
5	0.04217629692113033
6	0.08051838503124881
7	0.13228020397990875
8	0.12461178635788506
9	0.1361144127909206
10	0.15336835244047392
11	0.16295387446800352
12	0.14761703922395614
13	0.14761703922395614
14	0.13419730838541466
15	0.15911966565699168
16	0.1380315171964265
17	0.1437828304129443
18	0.11886047314136727
19	0.10927495111383767
20	0.10160653349181396
21	0.10160653349181396
22	0.1131091599248495
23	0.08243548943675473
24	0.09968942908630805
25	0.10160653349181396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	52162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.23342663241441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70483740732584	40.065564970668305
2	12.823900756074286	13.396725585675394
3	4.121705938486383	6.458724742149458
4	1.6552888497394114	3.4584563475326866
5	1.108419584526169	2.894827652313945
6	0.693679806210086	2.1739963958437176
7	0.4661234676649783	1.7043058164947662
8	0.4147397783160831	1.733062382577355
9	0.32665345371797694	1.535600628810245
>10	1.526829626367173	14.869061769103945
>50	0.07707553402334288	2.7280395690349297
>100	0.08074579754826397	8.981634139795252
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	450	0.8626969824776657	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	416	0.7975154326904644	No Hit
TATCAACGCAGAGTACTTTTTTTTT	376	0.7208312564702274	No Hit
GATATACACTGTTCTACAAATCCCG	251	0.48119320578198693	No Hit
GTCCTACAGTGTGCATTTCTCATTT	247	0.47352478815996313	No Hit
GTCCTACAGTGGACATTTCTAAATT	236	0.452436639699398	No Hit
GTGTATATCAATGAGTTACAATGAG	221	0.42368007361680915	No Hit
GGTATCAACGCAGAGTACTTTTTTT	217	0.41601165599478546	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	214	0.41026034277826773	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	201	0.38533798550669074	No Hit
CTGTAGGACGTGGAATATGGCAAGA	191	0.3661669414516315	No Hit
TTGTAGAACAGTGTATATCAATGAG	190	0.36424983704612557	No Hit
GTACATGGGGTGGTATCAACGCAAA	174	0.33357616655803074	No Hit
CTTTAGGACGTGAAATATGGCGAGG	173	0.33165906215252483	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	167	0.32015643571948926	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	155	0.2971511828534182	No Hit
GTTCTACAAATCCCGTTTCCAACGA	148	0.28373145201487676	No Hit
CTGTAGAACAGATTAGATGAGTGAG	146	0.2798972432038649	No Hit
GTTCTACAGTGTGGTTTTTATCATT	145	0.2779801387983589	No Hit
CTGTAGGACCTGGAATATGGCGAGA	135	0.2588090947432997	No Hit
GTATCAACGCAGAGTACATGGGGTG	120	0.23005252866071085	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	112	0.21471569341666347	No Hit
GTGTATATCAATGAGTTACAATGAA	99	0.18979333614508645	No Hit
GGTATCAACGCAGAGTACATGGGGT	83	0.15911966565699168	No Hit
TTGATATACACTGTTCTACAAATCC	81	0.15528545684597983	No Hit
GTACATGGGTGGTATCAACGCAAAA	80	0.15336835244047392	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	78	0.14953414362946205	No Hit
GATTAGATGAGTGAGTTACACTGAA	77	0.14761703922395614	No Hit
TATCAACGCAGAGTACATGGGGTGG	76	0.1456999348184502	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	75	0.1437828304129443	No Hit
CTGAAGGACCTGGAATATGGCGAGA	72	0.1380315171964265	No Hit
ACGCAGAGTACATGGGGTGGTATCA	71	0.1361144127909206	No Hit
ATTTAGAAATGTCCACTGTAGGACG	64	0.12269468195237913	No Hit
GAGTTACACTGAAAAACACATTCGT	61	0.11694336873586135	No Hit
GTATATCAATGAGTTACAATGAGAA	61	0.11694336873586135	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	60	0.11502626433035543	No Hit
CTGTAGGACATGGAATATGGCAAGA	59	0.1131091599248495	No Hit
GTACATGGGAGTGGTATCAACGCAA	56	0.10735784670833173	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	56	0.10735784670833173	No Hit
GTAGAACAGATTAGATGAGTGAGTT	55	0.10544074230282581	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	55	0.10544074230282581	No Hit
GATATACACTGTTCTACAATGCCGG	53	0.10160653349181396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTGG	45	1.5981459E-6	19.009644	7
TAGGACC	40	1.2514933E-5	18.99133	4
TTGTAGA	35	9.885783E-5	18.954807	1
CGTGGAA	50	4.3721284E-6	17.108677	9
ACGTGGA	55	1.0817554E-5	15.553344	8
CTGTAGG	105	5.456968E-12	15.344368	1
TCCTAAA	40	0.005271522	14.216105	2
GTCCTAA	40	0.005271522	14.216105	1
TGTAGGA	120	3.6379788E-12	14.216105	2
GGACGTG	115	2.1827873E-11	14.050605	6
GAACAGT	55	1.8951263E-4	13.825194	6
AACAGTG	55	1.8951263E-4	13.825194	7
TACACTG	125	5.456968E-12	13.686944	5
CCTACAG	140	0.0	13.539147	3
ACTGTTC	100	9.1840775E-9	13.306749	8
AGGACCT	50	0.0014601503	13.306749	5
GTAGGAC	130	1.2732926E-11	13.12256	3
GGCAAGA	110	2.4738256E-9	12.96112	19
TGGCAAG	120	6.5847416E-10	12.673095	18
GGACCTG	60	3.9578334E-4	12.673095	6
>>END_MODULE