FastQCFastQC Report
Thu 2 Feb 2017
SRR4062146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52162
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.38725508991219665No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG1870.35849852382960773No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC1500.2875656608258886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.2741459299873471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.2607261991488057No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.2588090947432997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.226218319849699No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG1010.19362754495609832No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTCATTGTAACTCAT940.18020781411755685No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA820.15720256125148574No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC800.15336835244047392No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA780.14953414362946205No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA760.1456999348184502No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA750.1437828304129443No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA730.13994862160193244No Hit
CTGTAGAACAGATTAGATGAGTGAGTTACACTGAAAAACACATTCGTTGG720.1380315171964265No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT680.13036309957440284No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC620.11886047314136727No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA610.11694336873586135No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.1111920555193436No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.10927495111383767No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA550.10544074230282581No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC540.10352363789731989No Hit
GTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCACAC530.10160653349181396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG530.10160653349181396No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA530.10160653349181396No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.10160653349181396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACTG207.787468E-444.012
ATTTAGA207.787468E-444.01
GGACATG557.4578566E-1140.06
GACATGG404.0246232E-738.57
GTCCACT404.0246232E-738.511
TAGGACA553.2923708E-936.04
CTACACC250.002332274835.24
CCGTGCA250.002332274835.210
TGACCGT250.002332274835.27
ACCGTGC250.002332274835.29
CCACTGT250.002332274835.213
GACCGTG250.002332274835.28
TTTAGAA250.002332274835.22
AGCATAA250.002332274835.222
TCACTTG250.002332274835.229
TCCTGAC250.002332274835.24
GTAGCAT250.002332274835.220
GCATAAT250.002332274835.223
CATAATC250.002332274835.224
TAGGACC702.5465852E-1134.571434
Produced by FastQC (version 0.11.5)