##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	52162
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.937809133085388	32.0	32.0	32.0	32.0	32.0
2	31.360914075380546	32.0	32.0	32.0	32.0	32.0
3	31.55586442237644	32.0	32.0	32.0	32.0	32.0
4	31.698995437291515	32.0	32.0	32.0	32.0	32.0
5	31.69999233158238	32.0	32.0	32.0	32.0	32.0
6	35.315977148115486	36.0	36.0	36.0	36.0	36.0
7	35.32510256508569	36.0	36.0	36.0	36.0	36.0
8	35.283693109926766	36.0	36.0	36.0	36.0	36.0
9	35.31722326597907	36.0	36.0	36.0	36.0	36.0
10	35.2626049614662	36.0	36.0	36.0	36.0	36.0
11	35.30633411295579	36.0	36.0	36.0	36.0	36.0
12	35.29193665887044	36.0	36.0	36.0	36.0	36.0
13	35.27752003374104	36.0	36.0	36.0	36.0	36.0
14	35.26933399792953	36.0	36.0	36.0	36.0	36.0
15	35.277654231049425	36.0	36.0	36.0	36.0	36.0
16	35.27220965453779	36.0	36.0	36.0	36.0	36.0
17	35.248629270350065	36.0	36.0	36.0	36.0	36.0
18	35.277654231049425	36.0	36.0	36.0	36.0	36.0
19	35.16335646639316	36.0	36.0	36.0	36.0	36.0
20	35.08778421072812	36.0	36.0	36.0	36.0	36.0
21	35.045032782485336	36.0	36.0	36.0	36.0	36.0
22	35.05482918599747	36.0	36.0	36.0	36.0	36.0
23	35.06249760361949	36.0	36.0	36.0	36.0	36.0
24	35.03441202407883	36.0	36.0	36.0	36.0	36.0
25	35.019190215099115	36.0	36.0	36.0	36.0	36.0
26	34.99275334534719	36.0	36.0	36.0	36.0	36.0
27	34.991794793144436	36.0	36.0	36.0	36.0	36.0
28	34.889670641463134	36.0	36.0	36.0	36.0	36.0
29	34.80395690349297	36.0	36.0	36.0	32.0	36.0
30	34.73720332809325	36.0	36.0	36.0	32.0	36.0
31	34.7239561366512	36.0	36.0	36.0	32.0	36.0
32	34.65754764004448	36.0	36.0	36.0	32.0	36.0
33	34.64767455235612	36.0	36.0	36.0	32.0	36.0
34	34.60657183390207	36.0	36.0	36.0	32.0	36.0
35	34.56957171887581	36.0	36.0	36.0	32.0	36.0
36	34.461485372493385	36.0	36.0	36.0	32.0	36.0
37	34.35888194471071	36.0	36.0	36.0	32.0	36.0
38	34.29446723668571	36.0	36.0	36.0	32.0	36.0
39	34.09184847206779	36.0	36.0	36.0	32.0	36.0
40	34.1258003910893	36.0	36.0	36.0	32.0	36.0
41	34.12547448334036	36.0	36.0	36.0	32.0	36.0
42	34.05536597523101	36.0	36.0	36.0	32.0	36.0
43	34.07699091292512	36.0	36.0	36.0	32.0	36.0
44	33.852191250335494	36.0	36.0	36.0	32.0	36.0
45	33.80376519305241	36.0	36.0	36.0	32.0	36.0
46	33.511061692419766	36.0	36.0	36.0	21.0	36.0
47	33.45195736359802	36.0	36.0	36.0	21.0	36.0
48	33.35313063149419	36.0	36.0	36.0	21.0	36.0
49	33.22627583298186	36.0	36.0	36.0	14.0	36.0
50	32.9363521337372	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	3.0
22	10.0
23	15.0
24	45.0
25	93.0
26	226.0
27	407.0
28	746.0
29	1057.0
30	1446.0
31	1983.0
32	2882.0
33	4377.0
34	8649.0
35	30220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87535953978907	22.0	13.119846596356663	23.004793863854267
2	12.020705521472392	22.268021472392636	45.8646472392638	19.846625766871163
3	19.656071469652236	27.040757639661056	30.907557225566507	22.395613665120205
4	10.120394156665773	18.124305049653003	40.01380315171964	31.74149764196158
5	10.369617729381542	40.901422491468885	35.64663931597715	13.082320463172426
6	28.11625321114988	40.817069897626624	18.312181281392583	12.754495609830913
7	25.271270273379088	32.96652735707987	21.672865304244468	20.089337065296576
8	23.317740884168554	41.165982899428705	19.888041102718454	15.62823511368429
9	25.648939841263758	14.178904183121812	21.962348069475865	38.20980790613857
10	16.0845059621947	29.582838081361913	33.001035236378975	21.331620720064414
11	33.52248763467659	22.911314750201296	26.07453701928607	17.491660595836052
12	23.432767148498908	26.304589547946783	33.73336911928224	16.529274184272076
13	29.895326099459375	22.949656838311412	27.94179671024884	19.21322035198037
14	19.201717725547333	25.47640044476822	28.681799010774128	26.640082818910315
15	21.73421264522066	34.617154250220466	25.173497948698287	18.47513515586059
16	18.678348222844214	29.01345807292665	32.619531459683294	19.68866224454584
17	16.379740040642613	30.77911123039761	31.538284574977954	21.302864153981826
18	17.046892373758674	28.409570185192283	37.030788696752424	17.512748744296612
19	22.917066063417813	24.928108584793527	30.123461523714585	22.031363828074078
20	22.22882558184119	28.192937387370115	31.90253441202408	17.675702618764618
21	21.603849545646256	25.71412139105096	29.71511828534182	22.966910777960965
22	20.63762892527127	29.989264215329165	31.189371573175872	18.183735286223687
23	20.55327633142901	29.162992216556116	31.40408726659254	18.879644185422336
24	21.79022641437089	27.861812465251816	32.476371235214046	17.871589885163246
25	20.860013036309958	28.720141098884245	32.95694183505234	17.46290402975346
26	16.604041256086806	30.529887657681837	34.80694758636555	18.059123499865805
27	17.104405505923854	29.747709060235422	34.34109121582761	18.806794218013113
28	16.868601664046622	30.66216786166175	31.66673057014685	20.80249990414478
29	18.296844446148537	27.136612859936353	30.775277021586593	23.791265672328514
30	18.207089588006365	28.76670309234869	31.97791453384713	21.048292785797816
31	21.473486446071853	27.362831179786053	31.538284574977954	19.625397799164144
32	18.034201142594224	30.203979908745833	32.42590391472719	19.33591503393275
33	18.741612668225912	30.169472029446727	33.60492312411334	17.483992178214024
34	18.548340714326798	30.024347692720614	33.94298422192826	17.484327371024328
35	18.235497105172346	30.988075610597754	32.533261761435526	18.24316552279437
36	17.683371036386642	30.42444691537901	32.98953260994594	18.90264943828841
37	17.796821379958207	29.732942236536875	30.716435651156992	21.753800732347923
38	19.583221502243013	32.41440128829416	30.014186572600742	17.988190636862083
39	20.23311989570952	27.667650780261493	31.147195276254745	20.952034047774244
40	22.230742686246693	28.768068709021893	31.411755684214565	17.589432920516852
41	21.180169472029448	27.303400943215365	33.811970399907985	17.704459184847206
42	19.127684049079754	32.354294478527606	31.39187116564417	17.126150306748468
43	17.279855825456778	32.2763089783163	32.801625798040604	17.642209398186314
44	16.810138231177746	31.749074943921467	31.672386357100407	19.76840046780038
45	17.557851952683144	31.97338906037309	30.964934143676064	19.5038248432677
46	19.225091543490347	28.782040221621518	29.51055386208086	22.48231437280727
47	21.56208661643757	30.706849945361476	28.47913191848316	19.251931519717797
48	19.17022949059606	30.761709388600238	28.15046300734293	21.917598113460766
49	20.516114189305775	29.977568588354835	29.191510573438904	20.314806648900475
50	18.949023216579437	32.35175705987231	27.420869998658	21.278349724890244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	295.0
1	157.0
2	19.0
3	61.5
4	104.0
5	87.5
6	71.0
7	46.5
8	22.0
9	47.0
10	72.0
11	115.5
12	159.0
13	277.5
14	396.0
15	506.5
16	617.0
17	662.0
18	707.0
19	698.0
20	689.0
21	695.5
22	702.0
23	634.0
24	566.0
25	673.0
26	780.0
27	1147.0
28	1514.0
29	1956.0
30	2398.0
31	2631.5
32	2865.0
33	3325.0
34	3785.0
35	4277.0
36	4769.0
37	5026.5
38	5284.0
39	4869.0
40	4454.0
41	3964.0
42	3474.0
43	2899.5
44	2325.0
45	2265.0
46	2205.0
47	2097.5
48	1990.0
49	1947.0
50	1904.0
51	1957.0
52	2010.0
53	1870.5
54	1731.0
55	1615.0
56	1499.0
57	1391.0
58	1283.0
59	1167.5
60	1052.0
61	895.0
62	738.0
63	631.0
64	524.0
65	475.0
66	426.0
67	343.5
68	261.0
69	213.0
70	165.0
71	135.5
72	106.0
73	93.0
74	80.0
75	63.0
76	46.0
77	39.5
78	33.0
79	26.5
80	20.0
81	15.5
82	11.0
83	8.5
84	6.0
85	5.0
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023005252866071087
2	0.003834208811011848
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.001917104405505924
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.001917104405505924
31	0.0
32	0.0
33	0.0
34	0.001917104405505924
35	0.0
36	0.0
37	0.001917104405505924
38	0.0
39	0.0
40	0.0
41	0.0
42	0.003834208811011848
43	0.005751313216517772
44	0.005751313216517772
45	0.005751313216517772
46	0.001917104405505924
47	0.001917104405505924
48	0.005751313216517772
49	0.005751313216517772
50	0.001917104405505924
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	52162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.47459836662704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71646004664294	49.80253824623289
2	11.88167423591506	14.846056516237875
3	3.5534552596047626	6.660020704727579
4	1.4637289799926354	3.657835205705303
5	0.9727507057812692	3.0386104827268894
6	0.47563520314226093	1.7829070971205092
7	0.3682337056585246	1.6103677006249761
8	0.2301460660365779	1.1502626433035543
9	0.20252853811218852	1.1387600168705188
>10	1.049466061126795	11.558222460795214
>50	0.061372284276420774	2.5075725624017484
>100	0.024548913710568308	2.246846363252943
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	202	0.38725508991219665	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	187	0.35849852382960773	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	150	0.2875656608258886	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143	0.2741459299873471	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136	0.2607261991488057	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	135	0.2588090947432997	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.226218319849699	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	101	0.19362754495609832	No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTCTCATTGTAACTCAT	94	0.18020781411755685	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	82	0.15720256125148574	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC	80	0.15336835244047392	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	78	0.14953414362946205	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	76	0.1456999348184502	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	75	0.1437828304129443	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	73	0.13994862160193244	No Hit
CTGTAGAACAGATTAGATGAGTGAGTTACACTGAAAAACACATTCGTTGG	72	0.1380315171964265	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	68	0.13036309957440284	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	62	0.11886047314136727	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	61	0.11694336873586135	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58	0.1111920555193436	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.10927495111383767	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	55	0.10544074230282581	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	54	0.10352363789731989	No Hit
GTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCACAC	53	0.10160653349181396	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	53	0.10160653349181396	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	53	0.10160653349181396	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.10160653349181396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0019171044055059237	0.0
21	0.0	0.0	0.0	0.0019171044055059237	0.0
22	0.0	0.0	0.0	0.0038342088110118475	0.0
23	0.0	0.0	0.0	0.00958552202752962	0.0
24	0.0	0.0	0.0	0.011502626433035543	0.0
25	0.0	0.0	0.0	0.01533683524404739	0.0
26	0.0	0.0	0.0	0.023005252866071087	0.0
27	0.0	0.0	0.0	0.02492235727157701	0.0
28	0.0	0.0	0.0	0.026839461677082933	0.0
29	0.0	0.0	0.0	0.03642498370461255	0.0
30	0.0	0.0	0.0	0.05367892335416587	0.0
31	0.0	0.0	0.0	0.07668417622023696	0.0
32	0.0	0.0	0.0	0.11886047314136727	0.0
33	0.0	0.0	0.0	0.1437828304129443	0.0
34	0.0	0.0	0.0	0.16295387446800352	0.0
35	0.0	0.0	0.0	0.19937885817261608	0.0
36	0.0	0.0	0.0	0.23580384187722864	0.0
37	0.0	0.0	0.0	0.2703117211763353	0.0
38	0.0	0.0	0.0	0.32782485334151296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCACTG	20	7.787468E-4	44.0	12
ATTTAGA	20	7.787468E-4	44.0	1
GGACATG	55	7.4578566E-11	40.0	6
GACATGG	40	4.0246232E-7	38.5	7
GTCCACT	40	4.0246232E-7	38.5	11
TAGGACA	55	3.2923708E-9	36.0	4
CTACACC	25	0.0023322748	35.2	4
CCGTGCA	25	0.0023322748	35.2	10
TGACCGT	25	0.0023322748	35.2	7
ACCGTGC	25	0.0023322748	35.2	9
CCACTGT	25	0.0023322748	35.2	13
GACCGTG	25	0.0023322748	35.2	8
TTTAGAA	25	0.0023322748	35.2	2
AGCATAA	25	0.0023322748	35.2	22
TCACTTG	25	0.0023322748	35.2	29
TCCTGAC	25	0.0023322748	35.2	4
GTAGCAT	25	0.0023322748	35.2	20
GCATAAT	25	0.0023322748	35.2	23
CATAATC	25	0.0023322748	35.2	24
TAGGACC	70	2.5465852E-11	34.57143	4
>>END_MODULE