FastQCFastQC Report
Thu 2 Feb 2017
SRR4062145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671900
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32500.12163628878326285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT400.005279239714.25047815
GGTATCA10900.014.0273231
GTATCAA27850.012.6168791
CGAACGA853.9464794E-612.29406916
TCGCGTA1551.8189894E-1212.2598549
CGTCTTA1650.012.09131415
GTCTTAG3400.011.7312911
CGCGTAA1657.2759576E-1211.51640110
AATCGCT1501.7644197E-1011.40038315
CGTATAT2100.011.31075210
ATAGCGT2100.011.3107526
ACCGTCG3200.011.2831098
AGCGTAT2200.011.2291228
TAGCGTA2200.011.2291227
GCGTAAC1701.0913936E-1111.17789311
GACCCGT600.005872783311.0845376
AAATCGC1553.1286618E-1011.03283514
CCGATAA1053.4625846E-610.8587279
ACGAACG1053.467254E-610.85750815
AAGACGG3900.010.7185095
Produced by FastQC (version 0.11.5)