##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062145_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2671900
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224286088551217	32.0	32.0	32.0	32.0	32.0
2	30.740011976496127	32.0	32.0	32.0	32.0	32.0
3	30.765863243384857	32.0	32.0	32.0	32.0	32.0
4	30.76965043601931	32.0	32.0	32.0	32.0	32.0
5	30.680323739660917	32.0	32.0	32.0	32.0	32.0
6	34.32404880422172	36.0	36.0	36.0	32.0	36.0
7	34.22920580860062	36.0	36.0	36.0	32.0	36.0
8	34.20836221415472	36.0	36.0	36.0	32.0	36.0
9	34.29355889067705	36.0	36.0	36.0	32.0	36.0
10	33.98759983532318	36.0	36.0	36.0	32.0	36.0
11	34.34271230210712	36.0	36.0	36.0	32.0	36.0
12	34.11700400464089	36.0	36.0	36.0	32.0	36.0
13	34.20734982596654	36.0	36.0	36.0	32.0	36.0
14	34.124356076200456	36.0	36.0	36.0	32.0	36.0
15	34.05750215202665	36.0	36.0	36.0	32.0	36.0
16	34.067533216063474	36.0	36.0	36.0	32.0	36.0
17	34.032918148134286	36.0	36.0	36.0	32.0	36.0
18	34.03541936449717	36.0	36.0	36.0	32.0	36.0
19	34.010429656798536	36.0	36.0	36.0	32.0	36.0
20	34.0257060518732	36.0	36.0	36.0	32.0	36.0
21	34.00050974961638	36.0	36.0	36.0	32.0	36.0
22	33.94785021894532	36.0	36.0	36.0	32.0	36.0
23	33.92483813016954	36.0	36.0	36.0	32.0	36.0
24	33.909783674538716	36.0	36.0	36.0	32.0	36.0
25	33.519274673453346	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	38.0
5	196.0
6	569.0
7	136.0
8	460.0
9	369.0
10	213.0
11	61.0
12	94.0
13	114.0
14	327.0
15	479.0
16	787.0
17	1127.0
18	1572.0
19	2259.0
20	3568.0
21	5294.0
22	7969.0
23	11849.0
24	16771.0
25	24164.0
26	33143.0
27	43182.0
28	57110.0
29	75286.0
30	98003.0
31	132710.0
32	187058.0
33	264008.0
34	564034.0
35	1138950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24966016336112	17.29135976573728	11.37977418079908	26.079205890102518
2	16.541206209385038	19.209279121041988	38.017381697082705	26.23213297249027
3	19.278561764447197	22.895970447829903	28.22606511882061	29.599402668902293
4	12.541093411317398	15.667542652368924	35.57312933509055	36.21823460122314
5	14.560242903460102	36.77430344961849	33.78525806620692	14.880195580714483
6	34.717394780708176	35.27866170775396	16.621770056201594	13.382173455336268
7	30.628242925743297	30.35912387756226	20.117875866544228	18.894757330150213
8	27.953553180910973	33.85333478704444	18.831928141711355	19.361183890333233
9	26.854589955575275	14.552208046810833	18.55931390475962	40.033888092854276
10	15.946195815721456	27.27999409387663	31.617621983370665	25.156188107031248
11	36.92187993654242	21.237115565734555	22.085277967978133	19.755726529744898
12	23.92013692012905	23.926245410668574	29.163095573143178	22.9905220960592
13	29.52444193952621	19.852555416319408	24.981150656841525	25.641851987312858
14	23.130002873906687	19.696527443935402	25.3717999686006	31.801669713557306
15	25.035076989494893	27.494176320333864	22.10180121840073	25.36894547177052
16	25.488970574459398	26.184159744001917	23.63823034581474	24.68863933572395
17	23.89073753475579	26.280212244530883	25.244321034841978	24.58472918587135
18	24.420573907035543	25.74971880158895	25.892434957177663	23.93727233419785
19	25.380576021734463	25.445124562199428	25.19356132991722	23.980738086148886
20	25.45995079073475	25.015396626078513	25.013860709608394	24.51079187357834
21	26.126256396002006	24.731357613822517	24.640027631253137	24.502358358922336
22	25.48867543798522	24.907405117876298	25.120731138031	24.48318830610748
23	24.460701135346316	24.92821210112111	25.295485958938745	25.315600804593824
24	24.48798016317653	25.399577954273262	25.27925034249539	24.833191540054823
25	24.69744930641663	25.056209659595353	25.251791554128722	24.994549479859298
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190.0
1	190.0
2	496.0
3	802.0
4	802.0
5	802.0
6	1749.0
7	2696.0
8	2696.0
9	2696.0
10	3454.5
11	4213.0
12	4213.0
13	4213.0
14	6249.5
15	8286.0
16	8286.0
17	8286.0
18	13957.5
19	19629.0
20	19629.0
21	19629.0
22	30502.5
23	41376.0
24	41376.0
25	41376.0
26	59954.5
27	78533.0
28	78533.0
29	78533.0
30	98931.0
31	119329.0
32	119329.0
33	119329.0
34	144937.0
35	170545.0
36	170545.0
37	170545.0
38	196655.5
39	222766.0
40	222766.0
41	222766.0
42	252573.0
43	282380.0
44	282380.0
45	282380.0
46	310028.5
47	337677.0
48	337677.0
49	337677.0
50	347383.5
51	357090.0
52	357090.0
53	357090.0
54	337413.0
55	317736.0
56	317736.0
57	317736.0
58	290467.5
59	263199.0
60	263199.0
61	263199.0
62	229612.5
63	196026.0
64	196026.0
65	196026.0
66	160416.0
67	124806.0
68	124806.0
69	124806.0
70	94490.0
71	64174.0
72	64174.0
73	64174.0
74	47908.0
75	31642.0
76	31642.0
77	31642.0
78	24293.0
79	16944.0
80	16944.0
81	16944.0
82	11835.5
83	6727.0
84	6727.0
85	6727.0
86	4830.0
87	2933.0
88	2933.0
89	2933.0
90	1954.0
91	975.0
92	975.0
93	975.0
94	620.5
95	266.0
96	266.0
97	266.0
98	613.0
99	960.0
100	960.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001047943411055803
2	3.36838953553651E-4
3	5.613982559227516E-4
4	0.009094651745948576
5	0.03293536434746809
6	0.06328829671769154
7	0.10558029866387214
8	0.10737677308282495
9	0.11662113103035293
10	0.13035667502526294
11	0.13701860099554625
12	0.13035667502526294
13	0.12620232793143457
14	0.11448781765784648
15	0.13039410157565778
16	0.11714510273588083
17	0.12275908529510834
18	0.11100714847112542
19	0.09697219207305663
20	0.09296755118080767
21	0.09203188742093642
22	0.1027733073842584
23	0.08346120738051574
24	0.09435233354541712
25	0.09094651745948576
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2671900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.252727539789596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.25820381023009	34.11155442160481
2	16.18076358315936	15.938934802941956
3	6.257786827694321	9.24639208879538
4	2.989160263361669	5.888971840964721
5	1.6104028013426328	3.9658365201921306
6	0.9804475152664018	2.8973828601887877
7	0.6120821196632621	2.110269971022608
8	0.4100172588852158	1.615557467078793
9	0.2880554043731055	1.2768762913157234
>10	1.2661161337165654	10.705620962430986
>50	0.07912103817358461	2.699270950765019
>100	0.059225423310140066	5.843695130422502
>500	0.006329903790864568	2.0876988177729054
>1k	0.002287917032809709	1.6119378745037085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3250	0.12163628878326285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2455930236910064E-4
2	0.0	0.0	0.0	0.0	2.2455930236910064E-4
3	0.0	0.0	0.0	0.0	2.2455930236910064E-4
4	0.0	0.0	0.0	0.0	2.2455930236910064E-4
5	0.0	0.0	0.0	0.0	2.9941240315880087E-4
6	0.0	0.0	0.0	0.0	4.491186047382013E-4
7	0.0	0.0	0.0	0.0	4.491186047382013E-4
8	0.0	0.0	0.0	0.0	4.491186047382013E-4
9	0.0	0.0	0.0	0.0	4.491186047382013E-4
10	0.0	0.0	0.0	2.2455930236910064E-4	4.491186047382013E-4
11	0.0	0.0	0.0	2.2455930236910064E-4	4.491186047382013E-4
12	0.0	0.0	0.0	2.2455930236910064E-4	5.988248063176017E-4
13	0.0	0.0	0.0	2.6198585276395077E-4	7.485310078970022E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCT	40	0.0052792397	14.250478	15
GGTATCA	1090	0.0	14.027323	1
GTATCAA	2785	0.0	12.616879	1
CGAACGA	85	3.9464794E-6	12.294069	16
TCGCGTA	155	1.8189894E-12	12.259854	9
CGTCTTA	165	0.0	12.091314	15
GTCTTAG	340	0.0	11.731291	1
CGCGTAA	165	7.2759576E-12	11.516401	10
AATCGCT	150	1.7644197E-10	11.400383	15
CGTATAT	210	0.0	11.310752	10
ATAGCGT	210	0.0	11.310752	6
ACCGTCG	320	0.0	11.283109	8
AGCGTAT	220	0.0	11.229122	8
TAGCGTA	220	0.0	11.229122	7
GCGTAAC	170	1.0913936E-11	11.177893	11
GACCCGT	60	0.0058727833	11.084537	6
AAATCGC	155	3.1286618E-10	11.032835	14
CCGATAA	105	3.4625846E-6	10.858727	9
ACGAACG	105	3.467254E-6	10.857508	15
AAGACGG	390	0.0	10.718509	5
>>END_MODULE