FastQCFastQC Report
Thu 2 Feb 2017
SRR4062145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671900
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28500.024.7853891
GGTATCA13400.024.6332661
GATATAC4800.018.796551
TAGATCG1102.7995538E-617.9996245
GGACCGT1751.9281288E-1017.5996326
TACGAAT4750.017.13776641
CGGATCG1551.441731E-817.03190226
TACCGTC5300.016.6034287
GTCCTAA5450.016.554761
ATACCGT5200.016.4996576
TTAACGG1753.6325218E-916.34251635
ATACGAA5100.015.96134840
CGCAATA5100.015.96075136
TAACGGC1805.2750693E-915.88885436
ATCAACG44750.015.7806773
TCAACGC44850.015.7454924
ACCGTCG5200.015.653528
TAGGACG4500.015.6441184
CAACGCA45750.015.4838315
TATCAAC47150.015.3048892
Produced by FastQC (version 0.11.5)