##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2671900
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275523784572776	32.0	32.0	32.0	32.0	32.0
2	31.42798944571279	32.0	32.0	32.0	32.0	32.0
3	31.565541749316967	32.0	32.0	32.0	32.0	32.0
4	31.641581646019688	32.0	32.0	32.0	32.0	32.0
5	31.61445675362102	32.0	32.0	32.0	32.0	32.0
6	35.24678543358659	36.0	36.0	36.0	36.0	36.0
7	35.255416744638644	36.0	36.0	36.0	36.0	36.0
8	35.202199184101204	36.0	36.0	36.0	36.0	36.0
9	35.29129608144017	36.0	36.0	36.0	36.0	36.0
10	35.169182229873876	36.0	36.0	36.0	36.0	36.0
11	35.29889479396684	36.0	36.0	36.0	36.0	36.0
12	35.219148171713016	36.0	36.0	36.0	36.0	36.0
13	35.25845091507916	36.0	36.0	36.0	36.0	36.0
14	35.215598637673565	36.0	36.0	36.0	36.0	36.0
15	35.191016130843224	36.0	36.0	36.0	36.0	36.0
16	35.210909465174595	36.0	36.0	36.0	36.0	36.0
17	35.194195890564764	36.0	36.0	36.0	36.0	36.0
18	35.18687787716606	36.0	36.0	36.0	36.0	36.0
19	35.17606310116397	36.0	36.0	36.0	36.0	36.0
20	35.178484598974514	36.0	36.0	36.0	36.0	36.0
21	35.167397357685545	36.0	36.0	36.0	36.0	36.0
22	35.15689995883079	36.0	36.0	36.0	36.0	36.0
23	35.12013361278491	36.0	36.0	36.0	36.0	36.0
24	35.10959841311426	36.0	36.0	36.0	36.0	36.0
25	35.09421722369849	36.0	36.0	36.0	36.0	36.0
26	35.04798907144728	36.0	36.0	36.0	36.0	36.0
27	35.039043751637415	36.0	36.0	36.0	36.0	36.0
28	35.01771136644336	36.0	36.0	36.0	36.0	36.0
29	34.99542310715221	36.0	36.0	36.0	36.0	36.0
30	34.97872600022456	36.0	36.0	36.0	36.0	36.0
31	34.97799356263333	36.0	36.0	36.0	36.0	36.0
32	34.95524383397582	36.0	36.0	36.0	36.0	36.0
33	34.93079119727535	36.0	36.0	36.0	36.0	36.0
34	34.92196826228526	36.0	36.0	36.0	36.0	36.0
35	34.913617276095664	36.0	36.0	36.0	36.0	36.0
36	34.886227029454695	36.0	36.0	36.0	32.0	36.0
37	34.87589730154571	36.0	36.0	36.0	32.0	36.0
38	34.85965006175381	36.0	36.0	36.0	32.0	36.0
39	34.84603727684419	36.0	36.0	36.0	32.0	36.0
40	34.83745574310416	36.0	36.0	36.0	32.0	36.0
41	34.806621131030354	36.0	36.0	36.0	32.0	36.0
42	34.78317040308395	36.0	36.0	36.0	32.0	36.0
43	34.78087278715521	36.0	36.0	36.0	32.0	36.0
44	34.7428058684831	36.0	36.0	36.0	32.0	36.0
45	34.720764250159064	36.0	36.0	36.0	32.0	36.0
46	34.70579961824919	36.0	36.0	36.0	32.0	36.0
47	34.67765784647629	36.0	36.0	36.0	32.0	36.0
48	34.65591713761743	36.0	36.0	36.0	32.0	36.0
49	34.641237696021555	36.0	36.0	36.0	32.0	36.0
50	34.22593135970658	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	19.0
21	46.0
22	159.0
23	424.0
24	1189.0
25	2832.0
26	6179.0
27	11586.0
28	20059.0
29	32897.0
30	49118.0
31	73695.0
32	112395.0
33	191741.0
34	442975.0
35	1726578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.278728780277056	17.66311337040502	11.799332170490832	26.25882567882709
2	15.999851782872387	19.73840425547842	37.78230497096292	26.479438990686276
3	18.50973018370503	23.33900472025067	28.585249703206568	29.566015392837734
4	12.378279410366714	16.083847512143056	35.72168548350274	35.8161875939875
5	14.275240194333694	37.06775592939703	33.661115184368875	14.995888691900404
6	34.83061079518873	35.46229753126434	16.49436691725046	13.21272475629647
7	30.68670234664471	30.297279089786294	20.085781653505	18.930236910064
8	28.040083835472885	34.01283730678543	18.658557580747782	19.2885212769939
9	27.114188405254687	14.258617463228415	18.356562745611736	40.27063138590516
10	15.90696354914613	27.27303689248733	31.734844767710157	25.085154790656382
11	37.19446835585164	21.09704704517385	22.037389123844456	19.671095475130056
12	24.07432912908417	23.772184587746548	29.191736217672815	22.961750065496464
13	29.74448894045436	19.613757999925145	25.020023204461246	25.62172985515925
14	23.222014296942252	19.57475953441371	25.43684269620869	31.766383472435344
15	25.16157041805457	27.37149593921928	22.07661214865826	25.39032149406789
16	25.538075179133834	26.065380807931476	23.731260888848134	24.665283124086553
17	23.998502937984206	26.254013997529846	25.158014895767057	24.589468168718888
18	24.564083121467633	25.654619171322846	25.902046373793603	23.879251333415922
19	25.546053370260864	25.248400014970617	25.182379580074105	24.023167034694414
20	25.61409759690526	24.876229841969426	25.100827579599144	24.40884498152617
21	26.277490279385564	24.63622315065053	24.635699178748897	24.450587391215013
22	25.60922938732737	24.740222313709346	25.142295744601217	24.508252554362066
23	24.729855159249972	24.69070698753696	25.296642838429584	25.28279501478349
24	24.642339449009068	25.17967705848735	25.378600886193485	24.799382606310097
25	24.909624068311068	24.858574158041392	25.318734456256703	24.91306731739084
26	24.554323303486818	25.52285047449523	25.539655045937486	24.38317117608047
27	25.040682842993416	25.18608564430844	24.99397429832388	24.779257214374255
28	24.709734694778852	25.056234107548807	25.429531271403583	24.804499926268758
29	24.56468129524648	25.328559190057387	25.29113254161001	24.815626973086122
30	24.631550739293846	25.34146445528494	25.513590835495986	24.51339396992523
31	24.973436456148164	25.037997376393907	25.024710926140436	24.963855241317493
32	24.894316105907333	25.17445561800423	24.956182587336347	24.97504568875209
33	24.657593718924257	24.90992361612471	25.27210047984598	25.16038218510505
34	24.979143937706898	25.085922485436313	25.464606448256568	24.470327128600218
35	25.236311592966192	24.952879902511178	25.455856066254075	24.35495243826856
36	24.570819908102745	25.304568252443865	25.230014480348608	24.894597359104786
37	25.29334422711361	25.12267666618885	25.0515651824702	24.532413924227345
38	24.745387177663837	25.09461431939818	25.35929488379056	24.800703619147423
39	25.146637883025036	25.05917164192511	24.90516069535849	24.889029779691366
40	25.36542389171439	25.12649252572236	25.18603823425828	24.322045348304968
41	24.512495723975007	25.474666844319753	25.508425921282218	24.504411510423022
42	25.2669653179948	25.413271781566582	25.093822902931333	24.225939997507286
43	24.928559478374716	24.8640337629683	25.31069843098462	24.89670832767236
44	24.618534892529542	25.321655331004816	25.146006219019064	24.913803557446577
45	24.851217265646376	25.458025509675135	25.10153081255715	24.58922641212134
46	24.676871346627014	25.31871223116901	24.940097595445245	25.06431882675873
47	24.9134783625731	25.177150877192982	25.251443274853802	24.657927485380114
48	25.268441517757058	25.463440340652838	24.657807008055585	24.61031113353452
49	24.82846898368902	25.501755357771106	24.901602652873322	24.76817300566655
50	24.896794510478493	25.644664715961273	24.817860556751977	24.640680216808253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	35.5
2	45.0
3	131.0
4	217.0
5	236.0
6	255.0
7	230.0
8	205.0
9	295.5
10	386.0
11	601.0
12	816.0
13	1551.5
14	2287.0
15	2961.5
16	3636.0
17	3962.5
18	4289.0
19	4733.0
20	5177.0
21	6560.5
22	7944.0
23	9728.5
24	11513.0
25	15006.0
26	18499.0
27	23736.0
28	28973.0
29	34268.5
30	39564.0
31	44471.5
32	49379.0
33	56349.0
34	63319.0
35	71398.0
36	79477.0
37	91367.0
38	103257.0
39	111864.5
40	120472.0
41	129958.5
42	139445.0
43	145569.5
44	151694.0
45	162165.5
46	172637.0
47	181482.5
48	190328.0
49	197245.0
50	204162.0
51	200232.0
52	196302.0
53	194428.5
54	192555.0
55	190615.5
56	188676.0
57	182951.5
58	177227.0
59	162478.5
60	147730.0
61	130052.5
62	112375.0
63	97256.5
64	82138.0
65	69365.5
66	56593.0
67	48757.0
68	40921.0
69	35848.0
70	30775.0
71	24185.5
72	17596.0
73	14945.0
74	12294.0
75	9452.5
76	6611.0
77	5726.0
78	4841.0
79	4091.5
80	3342.0
81	2555.5
82	1769.0
83	1450.5
84	1132.0
85	849.0
86	566.0
87	407.0
88	248.0
89	159.5
90	71.0
91	56.0
92	41.0
93	34.5
94	28.0
95	30.0
96	32.0
97	29.5
98	27.0
99	17.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032149406789176244
2	0.005389423256858416
3	2.9941240315880087E-4
4	7.485310078970022E-5
5	3.742655039485011E-5
6	2.2455930236910064E-4
7	0.0
8	0.0
9	0.0
10	3.742655039485011E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.613982559227516E-4
17	0.0
18	1.1227965118455032E-4
19	0.0
20	4.491186047382013E-4
21	3.742655039485011E-5
22	0.0
23	0.0
24	8.608106590815524E-4
25	1.871327519742505E-4
26	2.9941240315880087E-4
27	4.491186047382013E-4
28	0.0012725027134249036
29	2.6198585276395077E-4
30	9.730903102661027E-4
31	1.871327519742505E-4
32	6.362513567124518E-4
33	3.742655039485011E-5
34	5.613982559227516E-4
35	1.4970620157940044E-4
36	1.1227965118455032E-4
37	0.0014596354653991543
38	0.0
39	4.491186047382013E-4
40	1.1227965118455032E-4
41	9.730903102661027E-4
42	0.004154347093828362
43	0.0036303753883004603
44	0.0035929488379056102
45	0.0019087540701373555
46	0.0020210337213219056
47	9.356637598712527E-4
48	0.0033309629851416595
49	0.002994124031588008
50	0.0017216213181631048
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2671900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.68330467512709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.14808512005692	33.85822076046283
2	16.724384738149173	16.618454048990223
3	6.585960409273954	9.816368327768478
4	3.15865546452951	6.277297672318989
5	1.6834477511253225	4.181962376190847
6	0.9803340620501262	2.922374153294483
7	0.6223698454813733	2.164497345756399
8	0.431486720300525	1.7150148950369863
9	0.293762497865853	1.3135582515235995
>10	1.2406736621930454	10.536722174156173
>50	0.07359542915962189	2.5407520285604392
>100	0.04998882463336903	4.98089411156298
>500	0.005139294920177263	1.6802311308393114
>1k	0.0021161802612094534	1.3936527235383527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.742655039485011E-5
2	0.0	0.0	0.0	0.0	3.742655039485011E-5
3	0.0	0.0	0.0	0.0	3.742655039485011E-5
4	0.0	0.0	0.0	0.0	3.742655039485011E-5
5	0.0	0.0	0.0	0.0	3.742655039485011E-5
6	0.0	0.0	0.0	0.0	1.4970620157940044E-4
7	0.0	0.0	0.0	0.0	1.4970620157940044E-4
8	0.0	0.0	0.0	0.0	1.4970620157940044E-4
9	0.0	0.0	0.0	0.0	2.9941240315880087E-4
10	0.0	0.0	0.0	2.2455930236910064E-4	2.9941240315880087E-4
11	0.0	0.0	0.0	2.2455930236910064E-4	2.9941240315880087E-4
12	0.0	0.0	0.0	2.2455930236910064E-4	5.239717055279015E-4
13	0.0	0.0	0.0	2.2455930236910064E-4	6.73677907107302E-4
14	0.0	0.0	0.0	2.2455930236910064E-4	6.73677907107302E-4
15	0.0	0.0	0.0	2.6198585276395077E-4	8.233841086867024E-4
16	0.0	0.0	0.0	2.6198585276395077E-4	9.356637598712527E-4
17	0.0	0.0	0.0	3.36838953553651E-4	9.730903102661028E-4
18	0.0	0.0	0.0	3.742655039485011E-4	0.001047943411055803
19	0.0	0.0	0.0	3.742655039485011E-4	0.001047943411055803
20	0.0	0.0	0.0	4.491186047382013E-4	0.0011227965118455032
21	0.0	0.0	0.0	4.865451551330514E-4	0.0011602230622403532
22	3.742655039485011E-5	0.0	0.0	5.239717055279015E-4	0.0011976496126352035
23	3.742655039485011E-5	0.0	0.0	5.988248063176017E-4	0.0013099292638197536
24	3.742655039485011E-5	0.0	0.0	7.485310078970022E-4	0.0013099292638197536
25	3.742655039485011E-5	0.0	0.0	9.730903102661028E-4	0.0013099292638197536
26	3.742655039485011E-5	0.0	0.0	0.001047943411055803	0.001422208915004304
27	3.742655039485011E-5	0.0	0.0	0.0012350761630300535	0.0014596354653991543
28	3.742655039485011E-5	0.0	0.0	0.001422208915004304	0.0014596354653991543
29	3.742655039485011E-5	0.0	0.0	0.0016841947677682548	0.0014596354653991543
30	3.742655039485011E-5	0.0	0.0	0.0022081664732961563	0.0014596354653991543
31	3.742655039485011E-5	0.0	0.0	0.0029192709307983085	0.0015344885661888543
32	3.742655039485011E-5	0.0	0.0	0.004977731202515065	0.0015344885661888543
33	3.742655039485011E-5	0.0	0.0	0.006399940117519368	0.0015344885661888543
34	3.742655039485011E-5	0.0	0.0	0.008233841086867023	0.0017964744189528051
35	3.742655039485011E-5	0.0	0.0	0.00995546240503013	0.0017964744189528051
36	3.742655039485011E-5	0.0	0.0	0.013211572289382087	0.0017964744189528051
37	3.742655039485011E-5	0.0	0.0	0.01751562558478985	0.0017964744189528051
38	3.742655039485011E-5	0.0	0.0	0.023017328492832816	0.0018713275197425054
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2850	0.0	24.785389	1
GGTATCA	1340	0.0	24.633266	1
GATATAC	480	0.0	18.79655	1
TAGATCG	110	2.7995538E-6	17.999624	5
GGACCGT	175	1.9281288E-10	17.599632	6
TACGAAT	475	0.0	17.137766	41
CGGATCG	155	1.441731E-8	17.031902	26
TACCGTC	530	0.0	16.603428	7
GTCCTAA	545	0.0	16.55476	1
ATACCGT	520	0.0	16.499657	6
TTAACGG	175	3.6325218E-9	16.342516	35
ATACGAA	510	0.0	15.961348	40
CGCAATA	510	0.0	15.960751	36
TAACGGC	180	5.2750693E-9	15.888854	36
ATCAACG	4475	0.0	15.780677	3
TCAACGC	4485	0.0	15.745492	4
ACCGTCG	520	0.0	15.65352	8
TAGGACG	450	0.0	15.644118	4
CAACGCA	4575	0.0	15.483831	5
TATCAAC	4715	0.0	15.304889	2
>>END_MODULE