FastQCFastQC Report
Thu 2 Feb 2017
SRR4062144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050214
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20490.19510309327432315No Hit
TATCAACGCAGAGTACTTTTTTTTT12890.12273688981483774No Hit
GTACATGGAAGCAGTGGTATCAACG10920.10397880812862903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG653.366964E-614.6164855
CTAATGT400.005276621314.2503934
CGCATCG1650.013.81856313
AACGCCG1850.013.3523575
CGCCAGT1850.013.35108418
TCGCGTA500.00149759113.3016369
TTAACGA655.447777E-513.1542094
TAGGAGT655.447777E-513.1542094
ATACCGT1450.013.1056856
CGTCGTA1251.0186341E-1012.92220610
TACCGTC1251.0186341E-1012.9222067
ACAGCGT604.084166E-412.6688298
ACCGTCG1207.366907E-1012.6688298
CCGTCCA751.4756286E-512.6682259
CGCCCCT751.47635255E-512.6676215
ACGGTAT1301.9645086E-1012.4246059
ATCGCCA1850.012.32466516
CTAGCCT550.003066667412.0912434
GTAACAC550.00307105212.0889373
GCATCGC1900.012.00033114
Produced by FastQC (version 0.11.5)