##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062144_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050214
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23098054301314	32.0	32.0	32.0	32.0	32.0
2	30.813224733244844	32.0	32.0	32.0	32.0	32.0
3	30.83933274551663	32.0	32.0	32.0	32.0	32.0
4	30.831355323772108	32.0	32.0	32.0	32.0	32.0
5	30.78316704976319	32.0	32.0	32.0	32.0	32.0
6	34.43002283344156	36.0	36.0	36.0	32.0	36.0
7	34.35896874351322	36.0	36.0	36.0	32.0	36.0
8	34.32303606693493	36.0	36.0	36.0	32.0	36.0
9	34.42408309163656	36.0	36.0	36.0	32.0	36.0
10	34.1429222996456	36.0	36.0	36.0	32.0	36.0
11	34.42611220189409	36.0	36.0	36.0	32.0	36.0
12	34.26113820611799	36.0	36.0	36.0	32.0	36.0
13	34.32978802415508	36.0	36.0	36.0	32.0	36.0
14	34.24244201657948	36.0	36.0	36.0	32.0	36.0
15	34.200085887257266	36.0	36.0	36.0	32.0	36.0
16	34.20199978290139	36.0	36.0	36.0	32.0	36.0
17	34.14589502710876	36.0	36.0	36.0	32.0	36.0
18	34.14204152677454	36.0	36.0	36.0	32.0	36.0
19	34.14298800054084	36.0	36.0	36.0	32.0	36.0
20	34.12508593486661	36.0	36.0	36.0	32.0	36.0
21	34.091677505727404	36.0	36.0	36.0	32.0	36.0
22	34.06091806050957	36.0	36.0	36.0	32.0	36.0
23	34.03555275401013	36.0	36.0	36.0	32.0	36.0
24	34.00525607161969	36.0	36.0	36.0	32.0	36.0
25	33.64876015745362	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	17.0
5	78.0
6	232.0
7	50.0
8	182.0
9	140.0
10	80.0
11	27.0
12	40.0
13	51.0
14	232.0
15	285.0
16	449.0
17	580.0
18	826.0
19	1144.0
20	1555.0
21	2106.0
22	3239.0
23	4614.0
24	6519.0
25	8839.0
26	12173.0
27	15568.0
28	20290.0
29	26551.0
30	34506.0
31	47013.0
32	66945.0
33	95624.0
34	213602.0
35	486657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.040677811168116	17.53602157295154	11.64011944346051	25.783181172419834
2	16.37440821472236	19.897887283710336	37.80179620876547	25.925908292801836
3	18.80821476929288	23.819326874917873	28.696179606667645	28.676278749121604
4	12.765106461593549	16.02537670329503	35.778218583616564	35.431298251494866
5	14.568067278118512	36.65339176702218	33.50761203256462	15.270928922294688
6	33.64564167405909	35.3532215909145	17.1866141589733	13.8145225760531
7	29.922416480230453	30.717220986728154	20.62335627126302	18.73700626177837
8	27.759554715419952	33.30702348916196	19.426006899661306	19.507414895756774
9	27.04773780719166	15.021955539793582	18.929812820204816	39.000493832809944
10	16.18650434968326	26.907300831798263	31.419358283467393	25.48683653505109
11	35.99670455726547	21.714927039698832	22.71776764268094	19.570600760354758
12	24.460918690853614	24.03956997449405	28.809706562418057	22.68980477223427
13	29.018070388037103	20.267761714575013	25.20072117561923	25.513446721768652
14	23.32632971776446	20.32370431523134	25.40806229277528	30.94190367422892
15	24.811785614852727	27.349724066651156	22.666961605790977	25.17152871270514
16	25.19045253868396	26.259617960453753	24.001643549877066	24.548285950985225
17	23.60748825431692	26.416918359875524	25.596341295991216	24.37925208981634
18	24.19706623655422	25.622290348780012	26.465737806807827	23.714905607857943
19	25.246832565600418	25.709092607365854	25.451656654209476	23.592418172824257
20	25.29194968925097	25.171004890210906	25.40374499994758	24.133300420590544
21	25.920807724234677	24.76066671177744	24.76619436960398	24.5523311943839
22	25.242243721107567	25.338226183100605	24.94247724348282	24.47705285230901
23	24.090183087907356	25.21709049185577	25.59123327983898	25.101493140397892
24	24.417118507243902	25.717218485608928	25.43072185523247	24.434941151914696
25	24.598438164147158	25.148626007597606	25.416810416305307	24.83612541194993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	533.0
1	533.0
2	567.5
3	602.0
4	602.0
5	602.0
6	1318.0
7	2034.0
8	2034.0
9	2034.0
10	2164.5
11	2295.0
12	2295.0
13	2295.0
14	3017.5
15	3740.0
16	3740.0
17	3740.0
18	6087.5
19	8435.0
20	8435.0
21	8435.0
22	13006.0
23	17577.0
24	17577.0
25	17577.0
26	25293.5
27	33010.0
28	33010.0
29	33010.0
30	41502.0
31	49994.0
32	49994.0
33	49994.0
34	59630.0
35	69266.0
36	69266.0
37	69266.0
38	78869.5
39	88473.0
40	88473.0
41	88473.0
42	99199.0
43	109925.0
44	109925.0
45	109925.0
46	123885.5
47	137846.0
48	137846.0
49	137846.0
50	138307.0
51	138768.0
52	138768.0
53	138768.0
54	129186.0
55	119604.0
56	119604.0
57	119604.0
58	109149.5
59	98695.0
60	98695.0
61	98695.0
62	85912.5
63	73130.0
64	73130.0
65	73130.0
66	59847.5
67	46565.0
68	46565.0
69	46565.0
70	35448.0
71	24331.0
72	24331.0
73	24331.0
74	18462.5
75	12594.0
76	12594.0
77	12594.0
78	9944.5
79	7295.0
80	7295.0
81	7295.0
82	5141.0
83	2987.0
84	2987.0
85	2987.0
86	2202.5
87	1418.0
88	1418.0
89	1418.0
90	978.0
91	538.0
92	538.0
93	538.0
94	340.5
95	143.0
96	143.0
97	143.0
98	279.5
99	416.0
100	416.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.521868876248079E-4
2	1.9043737752496158E-4
3	7.617495100998463E-4
4	0.011140586585210253
5	0.03456438402078053
6	0.06827179984269872
7	0.10959671076561539
8	0.11207239667343988
9	0.1214990468609255
10	0.13349660164499808
11	0.1421615023223838
12	0.13701969312920986
13	0.13054482229336115
14	0.11730942455537632
15	0.1342583511550979
16	0.12045164128453818
17	0.12549823178894967
18	0.11292936487230221
19	0.09721828122649288
20	0.09283822154341877
21	0.08998166088054435
22	0.10131268484327956
23	0.08398288348850806
24	0.09398084580856854
25	0.08912469268168202
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1050214.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.768544755394046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06195673895489	49.26017146050996
2	11.93984722654981	14.511342811183193
3	3.181809617485656	5.800618204299608
4	1.2521167165245273	3.043572429083911
5	0.6714694951147371	2.0402112032880875
6	0.40965461224354344	1.4936468783025216
7	0.26368106339174163	1.1216460151309662
8	0.1826707167433812	0.8880506900736055
9	0.14254037655724772	0.7795774127042846
>10	0.7481813510021692	8.451710786206295
>50	0.07815813262231301	3.3734035891792717
>100	0.06414095613944791	7.4904671302607015
>500	0.003301372086872694	1.322369614563345
>1k	4.7162458383895626E-4	0.4232117752142478
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2049	0.19510309327432315	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1289	0.12273688981483774	No Hit
GTACATGGAAGCAGTGGTATCAACG	1092	0.10397880812862903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.521868876248079E-5	0.0	0.0	0.0
6	0.0	9.521868876248079E-5	0.0	0.0	0.0
7	0.0	9.521868876248079E-5	0.0	9.521868876248079E-5	0.0
8	0.0	9.521868876248079E-5	0.0	9.521868876248079E-5	0.0
9	0.0	9.521868876248079E-5	0.0	9.521868876248079E-5	0.0
10	0.0	9.521868876248079E-5	0.0	9.521868876248079E-5	0.0
11	0.0	9.521868876248079E-5	0.0	9.521868876248079E-5	0.0
12	0.0	9.521868876248079E-5	0.0	9.521868876248079E-5	0.0
13	0.0	1.9043737752496158E-4	0.0	9.521868876248079E-5	9.521868876248079E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGCG	65	3.366964E-6	14.616485	5
CTAATGT	40	0.0052766213	14.250393	4
CGCATCG	165	0.0	13.818563	13
AACGCCG	185	0.0	13.352357	5
CGCCAGT	185	0.0	13.351084	18
TCGCGTA	50	0.001497591	13.301636	9
TTAACGA	65	5.447777E-5	13.154209	4
TAGGAGT	65	5.447777E-5	13.154209	4
ATACCGT	145	0.0	13.105685	6
CGTCGTA	125	1.0186341E-10	12.922206	10
TACCGTC	125	1.0186341E-10	12.922206	7
ACAGCGT	60	4.084166E-4	12.668829	8
ACCGTCG	120	7.366907E-10	12.668829	8
CCGTCCA	75	1.4756286E-5	12.668225	9
CGCCCCT	75	1.47635255E-5	12.667621	5
ACGGTAT	130	1.9645086E-10	12.424605	9
ATCGCCA	185	0.0	12.324665	16
CTAGCCT	55	0.0030666674	12.091243	4
GTAACAC	55	0.003071052	12.088937	3
GCATCGC	190	0.0	12.000331	14
>>END_MODULE