FastQCFastQC Report
Thu 2 Feb 2017
SRR4062144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050214
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.12102295341711308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTG500.002580571721.99947533
GACGGTA2300.020.086488
TACGACG2350.019.6591055
ACGGTAT2350.019.6591059
CGACGGT2400.019.2495427
GTATAAT1051.7861148E-618.863881
TGTAGGA4200.018.858492
AATCGTA1303.5868652E-818.6149426
GTATCAA23000.018.4674631
ACGACGG2650.018.2637166
GTCTACG851.4266986E-418.1241171
TAACCTA1102.7963524E-617.999575
CTACGAC2950.017.8978794
GTCCTAA2600.017.7755781
CGTAAAC1551.4388206E-817.03185332
TAGGACG3400.016.8231284
GTCCTAC3800.016.7954711
TTGTCGA1451.2477358E-716.69084744
ATACGAA2400.016.50039340
GTCGTAA1602.1287633E-816.49960730
Produced by FastQC (version 0.11.5)