##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050214
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276591247117253	32.0	32.0	32.0	32.0	32.0
2	31.499143031801136	32.0	32.0	32.0	32.0	32.0
3	31.596407017998235	32.0	32.0	32.0	32.0	32.0
4	31.678634068865964	32.0	32.0	32.0	32.0	32.0
5	31.659802668789407	32.0	32.0	32.0	32.0	32.0
6	35.30463029439714	36.0	36.0	36.0	36.0	36.0
7	35.31434069627714	36.0	36.0	36.0	36.0	36.0
8	35.27356710156216	36.0	36.0	36.0	36.0	36.0
9	35.33972885526188	36.0	36.0	36.0	36.0	36.0
10	35.22918090979553	36.0	36.0	36.0	36.0	36.0
11	35.34690453564702	36.0	36.0	36.0	36.0	36.0
12	35.264270900978275	36.0	36.0	36.0	36.0	36.0
13	35.297168957945715	36.0	36.0	36.0	36.0	36.0
14	35.27102285819842	36.0	36.0	36.0	36.0	36.0
15	35.246608786399726	36.0	36.0	36.0	36.0	36.0
16	35.252842753953004	36.0	36.0	36.0	36.0	36.0
17	35.23935788325046	36.0	36.0	36.0	36.0	36.0
18	35.228689581361515	36.0	36.0	36.0	36.0	36.0
19	35.21940956795472	36.0	36.0	36.0	36.0	36.0
20	35.217648022212614	36.0	36.0	36.0	36.0	36.0
21	35.20013444878853	36.0	36.0	36.0	36.0	36.0
22	35.18108214135405	36.0	36.0	36.0	36.0	36.0
23	35.15219183899662	36.0	36.0	36.0	36.0	36.0
24	35.13441070105712	36.0	36.0	36.0	36.0	36.0
25	35.11143633583251	36.0	36.0	36.0	36.0	36.0
26	35.07913815660427	36.0	36.0	36.0	36.0	36.0
27	35.055886704995366	36.0	36.0	36.0	36.0	36.0
28	35.026154669429275	36.0	36.0	36.0	36.0	36.0
29	35.00685479340401	36.0	36.0	36.0	36.0	36.0
30	34.98319485362031	36.0	36.0	36.0	36.0	36.0
31	34.98898795864462	36.0	36.0	36.0	36.0	36.0
32	34.948492402500825	36.0	36.0	36.0	36.0	36.0
33	34.937069016409986	36.0	36.0	36.0	36.0	36.0
34	34.916123761442904	36.0	36.0	36.0	36.0	36.0
35	34.89236669859667	36.0	36.0	36.0	36.0	36.0
36	34.868655340721034	36.0	36.0	36.0	32.0	36.0
37	34.84183414047042	36.0	36.0	36.0	32.0	36.0
38	34.83126867476533	36.0	36.0	36.0	32.0	36.0
39	34.78277474876549	36.0	36.0	36.0	32.0	36.0
40	34.773167183069354	36.0	36.0	36.0	32.0	36.0
41	34.74614792794611	36.0	36.0	36.0	32.0	36.0
42	34.70108473130238	36.0	36.0	36.0	32.0	36.0
43	34.71079227662172	36.0	36.0	36.0	32.0	36.0
44	34.6574412453081	36.0	36.0	36.0	32.0	36.0
45	34.61365588346756	36.0	36.0	36.0	32.0	36.0
46	34.60392072472848	36.0	36.0	36.0	32.0	36.0
47	34.57480094533114	36.0	36.0	36.0	32.0	36.0
48	34.54060600982276	36.0	36.0	36.0	32.0	36.0
49	34.52958254222473	36.0	36.0	36.0	32.0	36.0
50	34.127724444732216	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	13.0
21	41.0
22	109.0
23	241.0
24	558.0
25	1402.0
26	2997.0
27	5513.0
28	9129.0
29	13777.0
30	20191.0
31	28483.0
32	42613.0
33	70056.0
34	158810.0
35	696275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72965239770716	18.03923884489436	12.139219985560228	26.09188877183825
2	15.810728712169833	20.255219710632915	37.48602585906448	26.44802571813277
3	18.25391611035095	24.090732070541883	29.020779594234693	28.63457222487248
4	12.542408063887992	16.25651177427817	35.91814231970086	35.28293784213298
5	14.251857240524313	36.82201913133895	33.50307651583392	15.423047112302827
6	33.816983095745705	35.45170532722534	16.971955106078884	13.759356470950069
7	29.87257835069805	30.81267246485002	20.61551264789843	18.699236536553503
8	27.766626611338264	33.282359595282486	19.414328889159734	19.53668490421952
9	27.146467291428223	14.626542780804675	18.861774838271057	39.36521508949605
10	16.171306364810153	26.917041511618606	31.521445193922755	25.39020692964849
11	36.37353910726766	21.557320698448127	22.60843980369715	19.46070039058706
12	24.528928893472084	23.88334556894649	28.902232213846148	22.68549332373528
13	29.365729270415365	20.003637353910726	25.27008781067478	25.360545564999132
14	23.2943000188533	20.090095923307057	25.563456590751983	31.05214746708766
15	24.906257200913338	27.322717084327575	22.762217986048558	25.00880772871053
16	25.341457469215506	25.98733198502386	24.16987556703269	24.501334978727943
17	23.74677922785261	26.208753644495314	25.621444772208328	24.423022355443745
18	24.289309481618456	25.362236731475868	26.744816041728757	23.603637745176922
19	25.403608601302786	25.47007130934391	25.659175805289024	23.467144284064283
20	25.44933784797736	24.834128728294857	25.69271727993967	24.023816143788114
21	26.002058630011245	24.541212116018368	25.060916214139418	24.39581303983097
22	25.264755564104078	25.030422371059615	25.355498974494722	24.34932309034159
23	24.158813497833297	24.904852634656017	25.855802584808984	25.080531282701703
24	24.483031472783036	25.347145187291293	25.731925740495466	24.437897599430208
25	24.80392072846175	24.820298358523463	25.71468835369587	24.66109255931892
26	24.116319593224212	25.400919816036794	26.219327563058815	24.26343302768018
27	24.66363870083126	25.424248483636607	25.454813799144933	24.4572990163872
28	24.324833886568666	25.007284423902053	25.898174228184338	24.769707461344943
29	24.413449526189098	25.225098266248207	25.818980620981748	24.542471586580945
30	24.55194334042403	25.121738484596296	26.0540353189196	24.272282856060073
31	24.846173622418373	24.77809200064749	25.47376239037907	24.90197198655507
32	24.333869418124237	25.287586161585285	25.52868148850655	24.849862931783925
33	24.295403495107173	24.920611191465333	25.94535764717757	24.838627666249923
34	24.671801546939022	25.1504709089576	26.0817194390037	24.09600810509968
35	25.132806392607975	24.952462024370295	25.81619157256671	24.09854001045502
36	24.319470735432468	25.317459712896063	25.693574249770524	24.669495301900948
37	25.12866707103755	25.081437567071134	25.252263635852906	24.537631726038406
38	24.469203419493553	25.29893907336981	25.627919643044176	24.60393786409246
39	24.946201133489744	25.035040677656234	25.198436881074986	24.820321307779032
40	25.172536592611777	25.116167021515658	25.76194139849859	23.949354987373976
41	24.441801584256087	25.255734810880504	25.952358054444403	24.350105550419013
42	25.119718598209246	25.678579964596242	25.374724687693718	23.826976749500794
43	24.615967670057856	25.07208343014287	25.62237423964836	24.68957466015091
44	24.388787688053423	25.30321367934814	25.527463727197674	24.78053490540076
45	24.384823259879678	25.63888682576229	25.500911267146964	24.475378647211066
46	24.66755983922894	25.1315715552153	25.48760270770309	24.713265897852672
47	24.92446741553348	25.219361098600373	25.499781472068893	24.356390013797256
48	25.038850778729582	25.861477929329673	24.79212928929737	24.307542002643377
49	24.59337806864938	25.503510360634447	25.295735861669034	24.60737570904714
50	24.41996872982531	26.044132462135856	24.931965469500152	24.60393333853868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	151.5
2	80.0
3	257.5
4	435.0
5	419.5
6	404.0
7	320.0
8	236.0
9	326.0
10	416.0
11	611.0
12	806.0
13	1388.5
14	1971.0
15	2527.5
16	3084.0
17	3182.5
18	3281.0
19	3097.0
20	2913.0
21	3424.0
22	3935.0
23	4382.5
24	4830.0
25	6169.0
26	7508.0
27	9793.5
28	12079.0
29	14240.5
30	16402.0
31	18089.5
32	19777.0
33	22649.5
34	25522.0
35	29386.5
36	33251.0
37	38353.0
38	43455.0
39	46501.5
40	49548.0
41	52690.0
42	55832.0
43	57086.0
44	58340.0
45	62896.0
46	67452.0
47	70897.0
48	74342.0
49	75810.0
50	77278.0
51	76035.5
52	74793.0
53	73751.0
54	72709.0
55	72353.0
56	71997.0
57	69632.5
58	67268.0
59	61312.0
60	55356.0
61	49093.5
62	42831.0
63	37057.5
64	31284.0
65	26298.0
66	21312.0
67	18384.0
68	15456.0
69	13939.5
70	12423.0
71	9911.0
72	7399.0
73	6480.5
74	5562.0
75	4224.0
76	2886.0
77	2502.0
78	2118.0
79	1813.0
80	1508.0
81	1203.5
82	899.0
83	719.5
84	540.0
85	405.5
86	271.0
87	188.0
88	105.0
89	67.0
90	29.0
91	24.0
92	19.0
93	17.5
94	16.0
95	12.5
96	9.0
97	13.0
98	17.0
99	12.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03199347942419355
2	0.005713121325748848
3	9.521868876248079E-5
4	9.521868876248079E-5
5	0.0
6	4.7609344381240395E-4
7	0.0
8	0.0
9	0.0
10	1.9043737752496158E-4
11	0.0
12	9.521868876248079E-5
13	0.0
14	0.0
15	0.0
16	9.521868876248079E-4
17	0.0
18	2.8565606628744235E-4
19	9.521868876248079E-5
20	1.9043737752496158E-4
21	9.521868876248079E-5
22	0.0
23	9.521868876248079E-5
24	4.7609344381240395E-4
25	9.521868876248079E-5
26	3.8087475504992315E-4
27	3.8087475504992315E-4
28	0.0026661232853494622
29	5.713121325748847E-4
30	0.0011426242651497694
31	3.8087475504992315E-4
32	6.665308213373656E-4
33	2.8565606628744235E-4
34	0.0010474055763872885
35	9.521868876248079E-5
36	1.9043737752496158E-4
37	0.002190029841537058
38	0.0
39	5.713121325748847E-4
40	1.9043737752496158E-4
41	4.7609344381240395E-4
42	0.004094403616786675
43	0.003999184928024193
44	0.004475278371836597
45	0.0026661232853494622
46	0.0023804672190620197
47	4.7609344381240395E-4
48	0.003999184928024193
49	0.0035230914842117895
50	0.0011426242651497694
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1050214.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.14509717066282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.11182636987513	51.21814157815696
2	12.054307462154618	15.223408320455986
3	3.2432688982850753	6.143895891983989
4	1.2432952806293185	3.140320052286593
5	0.6163129490025725	1.9458570526152599
6	0.3688925843606672	1.3976254850994758
7	0.2609483652430839	1.1534326915860105
8	0.1810353087713749	0.9145193730951531
9	0.12643295846760894	0.7185259303210388
>10	0.6720803134456633	7.836174077019462
>50	0.06558340456064783	2.9256065885429305
>100	0.05359378798511351	6.337315541053679
>500	0.0022709223929834542	0.9236718385277441
>1k	1.5139482619889696E-4	0.12150557925571961
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1271	0.12102295341711308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.9043737752496158E-4
13	0.0	0.0	0.0	0.0	1.9043737752496158E-4
14	0.0	0.0	0.0	0.0	1.9043737752496158E-4
15	0.0	0.0	0.0	0.0	1.9043737752496158E-4
16	0.0	0.0	0.0	0.0	1.9043737752496158E-4
17	0.0	0.0	0.0	0.0	1.9043737752496158E-4
18	0.0	0.0	0.0	9.521868876248079E-5	1.9043737752496158E-4
19	0.0	0.0	0.0	9.521868876248079E-5	1.9043737752496158E-4
20	0.0	0.0	0.0	2.8565606628744235E-4	1.9043737752496158E-4
21	0.0	0.0	0.0	3.8087475504992315E-4	1.9043737752496158E-4
22	0.0	0.0	0.0	0.0011426242651497694	1.9043737752496158E-4
23	0.0	0.0	0.0	0.001809155086487135	1.9043737752496158E-4
24	0.0	0.0	0.0	0.0023804672190620197	1.9043737752496158E-4
25	0.0	0.0	0.0	0.0025709045965869814	1.9043737752496158E-4
26	0.0	0.0	0.0	0.003046998040399385	1.9043737752496158E-4
27	0.0	0.0	0.0	0.004380059683074117	1.9043737752496158E-4
28	0.0	0.0	0.0	0.005903558703273809	1.9043737752496158E-4
29	0.0	0.0	0.0	0.007998369856048387	1.9043737752496158E-4
30	0.0	0.0	0.0	0.01209277347283506	1.9043737752496158E-4
31	0.0	0.0	0.0	0.02151942366032066	1.9043737752496158E-4
32	0.0	0.0	0.0	0.03180304204666858	1.9043737752496158E-4
33	0.0	0.0	0.0	0.03999184928024193	1.9043737752496158E-4
34	0.0	0.0	0.0	0.05141809193173963	1.9043737752496158E-4
35	0.0	0.0	0.0	0.06208258507313748	1.9043737752496158E-4
36	0.0	0.0	0.0	0.07646060707627207	1.9043737752496158E-4
37	0.0	0.0	0.0	0.1020744343533794	1.9043737752496158E-4
38	0.0	0.0	0.0	0.13359182033376055	1.9043737752496158E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTTG	50	0.0025805717	21.999475	33
GACGGTA	230	0.0	20.08648	8
TACGACG	235	0.0	19.659105	5
ACGGTAT	235	0.0	19.659105	9
CGACGGT	240	0.0	19.249542	7
GTATAAT	105	1.7861148E-6	18.86388	1
TGTAGGA	420	0.0	18.85849	2
AATCGTA	130	3.5868652E-8	18.61494	26
GTATCAA	2300	0.0	18.467463	1
ACGACGG	265	0.0	18.263716	6
GTCTACG	85	1.4266986E-4	18.124117	1
TAACCTA	110	2.7963524E-6	17.99957	5
CTACGAC	295	0.0	17.897879	4
GTCCTAA	260	0.0	17.775578	1
CGTAAAC	155	1.4388206E-8	17.031853	32
TAGGACG	340	0.0	16.823128	4
GTCCTAC	380	0.0	16.795471	1
TTGTCGA	145	1.2477358E-7	16.690847	44
ATACGAA	240	0.0	16.500393	40
GTCGTAA	160	2.1287633E-8	16.499607	30
>>END_MODULE