FastQCFastQC Report
Thu 2 Feb 2017
SRR4062143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456769
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42310.17221806364375325No Hit
TATCAACGCAGAGTACTTTTTTTTT25430.10350993520351325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9950.014.9817181
GTATCAA29950.012.8076541
TTGCGCG1700.012.29262718
GTATTAC1800.012.1322241
AGGCCCG5100.011.92303910
CGCATCG3350.011.91118913
CGAACGA1459.822543E-1111.79335716
GCGTTAT2350.011.7169671
CGCGTAA2550.011.55044510
TCGCGTA2550.011.5504459
TTTGCGC2000.011.39954817
GATATAC5000.011.3937411
TCCAACG2100.011.30815218
ATTTGCG1850.011.29754116
CGTTATT2450.011.241932
TGCGCGC1950.011.20308519
GCGTAAC2550.011.17807811
AACCGCG1451.2150849E-911.139767
GTATCGA600.00587190411.0847019
GTGCTAT2150.011.0404451
Produced by FastQC (version 0.11.5)