##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062143_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2456769 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26865041035604 32.0 32.0 32.0 32.0 32.0 2 30.8249391782459 32.0 32.0 32.0 32.0 32.0 3 30.84801175853326 32.0 32.0 32.0 32.0 32.0 4 30.85673703958329 32.0 32.0 32.0 32.0 32.0 5 30.781755631074798 32.0 32.0 32.0 32.0 32.0 6 34.442115233463134 36.0 36.0 36.0 32.0 36.0 7 34.368172180616085 36.0 36.0 36.0 32.0 36.0 8 34.34223241989784 36.0 36.0 36.0 32.0 36.0 9 34.45326931429044 36.0 36.0 36.0 32.0 36.0 10 34.161797466509874 36.0 36.0 36.0 32.0 36.0 11 34.43689618356468 36.0 36.0 36.0 32.0 36.0 12 34.27201946947393 36.0 36.0 36.0 32.0 36.0 13 34.351465278176335 36.0 36.0 36.0 32.0 36.0 14 34.26743173656131 36.0 36.0 36.0 32.0 36.0 15 34.214607071320096 36.0 36.0 36.0 32.0 36.0 16 34.21987740809168 36.0 36.0 36.0 32.0 36.0 17 34.159168403704214 36.0 36.0 36.0 32.0 36.0 18 34.16195173416793 36.0 36.0 36.0 32.0 36.0 19 34.16013512055875 36.0 36.0 36.0 32.0 36.0 20 34.13726483849316 36.0 36.0 36.0 32.0 36.0 21 34.122757980094995 36.0 36.0 36.0 32.0 36.0 22 34.09098331996211 36.0 36.0 36.0 32.0 36.0 23 34.06149703126342 36.0 36.0 36.0 32.0 36.0 24 34.036551258990976 36.0 36.0 36.0 32.0 36.0 25 33.68761898249286 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 30.0 5 203.0 6 463.0 7 108.0 8 432.0 9 333.0 10 183.0 11 63.0 12 90.0 13 115.0 14 316.0 15 500.0 16 788.0 17 1139.0 18 1527.0 19 2156.0 20 3222.0 21 4692.0 22 6969.0 23 10184.0 24 14269.0 25 20073.0 26 27601.0 27 35762.0 28 47636.0 29 62365.0 30 82481.0 31 112512.0 32 159097.0 33 227071.0 34 506196.0 35 1128189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.114482967476015 17.326205733530724 11.951768623554539 25.607542675438722 2 16.39013759563393 19.299086114905624 38.36734012709401 25.943436162366428 3 19.618324867538362 22.9424332725891 27.909607371367606 29.52963448850493 4 12.809174009415816 15.617757749322717 35.38832609744332 36.18474214381815 5 14.938568435882141 36.36508366297026 33.26241813366124 15.433929767486365 6 34.68623294213001 34.96917822864928 16.583233037843474 13.761355791377236 7 30.639046580575968 30.028449699204124 19.85297593144038 19.479527788779524 8 27.853446449236387 33.630262578846995 18.82275520349453 19.693535768422084 9 26.946327158682653 14.770341464050754 18.523381517777388 39.75994985948921 10 16.498140018479578 26.82791533303798 30.709686780497726 25.96425786798472 11 37.035969721635844 21.39808565283086 22.071649230099506 19.494295395433788 12 24.850458284831113 23.372281837834667 28.622626651928513 23.154633225405707 13 29.53131979380973 19.757537323895445 24.946406505231376 25.764736377063453 14 23.48041319118018 19.953235019293203 24.88686713341997 31.67948465610665 15 24.974332784869404 27.599174899399596 22.007777400541833 25.41871491518916 16 25.483862028719972 26.099004768731593 23.927931922301706 24.489201280246725 17 23.841610450518445 26.26832229387152 25.082271261441054 24.80779599416898 18 24.442096401340965 25.469335829340277 26.235985938364003 23.85258183095475 19 25.554851602940847 25.006651333628042 25.070740447238528 24.367756616192583 20 25.726163393282008 24.77853320209375 24.90723327836046 24.588070126263776 21 26.391791637639177 24.4086744317742 24.45849985944585 24.74103407114077 22 25.85957716769094 24.692300201404986 25.010726251793315 24.437396379110762 23 24.4463346365067 24.72672670470678 25.467771614285127 25.359167044501397 24 24.768664977020293 25.014289747600078 25.461254311877866 24.755790963501763 25 24.749144259059936 24.79257295899369 25.38081592179249 25.077466860153883 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 499.0 1 499.0 2 624.5 3 750.0 4 750.0 5 750.0 6 1583.0 7 2416.0 8 2416.0 9 2416.0 10 3113.0 11 3810.0 12 3810.0 13 3810.0 14 5265.5 15 6721.0 16 6721.0 17 6721.0 18 11359.5 19 15998.0 20 15998.0 21 15998.0 22 25577.5 23 35157.0 24 35157.0 25 35157.0 26 52466.0 27 69775.0 28 69775.0 29 69775.0 30 89886.5 31 109998.0 32 109998.0 33 109998.0 34 131962.5 35 153927.0 36 153927.0 37 153927.0 38 177347.5 39 200768.0 40 200768.0 41 200768.0 42 228882.5 43 256997.0 44 256997.0 45 256997.0 46 284250.0 47 311503.0 48 311503.0 49 311503.0 50 320868.5 51 330234.0 52 330234.0 53 330234.0 54 310349.0 55 290464.0 56 290464.0 57 290464.0 58 267820.5 59 245177.0 60 245177.0 61 245177.0 62 215219.0 63 185261.0 64 185261.0 65 185261.0 66 151428.5 67 117596.0 68 117596.0 69 117596.0 70 88927.5 71 60259.0 72 60259.0 73 60259.0 74 45332.5 75 30406.0 76 30406.0 77 30406.0 78 23760.5 79 17115.0 80 17115.0 81 17115.0 82 11818.5 83 6522.0 84 6522.0 85 6522.0 86 4800.0 87 3078.0 88 3078.0 89 3078.0 90 2046.5 91 1015.0 92 1015.0 93 1015.0 94 648.0 95 281.0 96 281.0 97 281.0 98 661.5 99 1042.0 100 1042.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0011397082916627488 2 2.0351933779691947E-4 3 8.140773511876779E-4 4 0.010745821035677346 5 0.03191183216655697 6 0.06203269416050105 7 0.1063592059326701 8 0.10782454516480793 9 0.11661658055763485 10 0.13228756956799764 11 0.13839314970190525 12 0.13322375852186347 13 0.12671113971236206 14 0.11380801369603735 15 0.13228756956799764 16 0.1166979882927536 17 0.12194878720791413 18 0.11055170429128665 19 0.09663098158597735 20 0.09056610531962915 21 0.08950780476308517 22 0.10216670757405355 23 0.08108210417829272 24 0.09003695504135717 25 0.08836809647142242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2456769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.03376722915041 #Duplication Level Percentage of deduplicated Percentage of total 1 71.2302011441951 33.502247003016365 2 15.508060041964008 14.588049723788476 3 5.6868611137430145 8.02423505664888 4 2.601461285088502 4.894260981539965 5 1.3393284403562646 3.149683105354881 6 0.8393414559338696 2.368643439850189 7 0.5490990312374361 1.8078337214681495 8 0.38466688696783935 1.4473866257925816 9 0.2664756616530181 1.128001881817971 >10 1.406766559123473 11.880103813162277 >50 0.09855526314573179 3.2103303788592865 >100 0.07453076573816494 7.232698303308667 >500 0.009276932197292007 2.9587188758484135 >1k 0.005375418656374138 3.807807089543893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4231 0.17221806364375325 No Hit TATCAACGCAGAGTACTTTTTTTTT 2543 0.10350993520351325 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.140773511876778E-5 2 0.0 0.0 0.0 0.0 8.140773511876778E-5 3 0.0 0.0 0.0 0.0 8.140773511876778E-5 4 0.0 0.0 0.0 0.0 8.140773511876778E-5 5 0.0 0.0 0.0 0.0 1.2211160267815166E-4 6 0.0 0.0 0.0 0.0 2.442232053563033E-4 7 0.0 0.0 0.0 0.0 2.442232053563033E-4 8 0.0 0.0 0.0 0.0 2.442232053563033E-4 9 0.0 0.0 0.0 0.0 2.442232053563033E-4 10 0.0 0.0 0.0 0.0 2.442232053563033E-4 11 0.0 0.0 0.0 0.0 2.442232053563033E-4 12 0.0 0.0 0.0 0.0 8.547812187470617E-4 13 0.0 0.0 0.0 0.0 0.0010175966889845972 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 995 0.0 14.981718 1 GTATCAA 2995 0.0 12.807654 1 TTGCGCG 170 0.0 12.292627 18 GTATTAC 180 0.0 12.132224 1 AGGCCCG 510 0.0 11.923039 10 CGCATCG 335 0.0 11.911189 13 CGAACGA 145 9.822543E-11 11.793357 16 GCGTTAT 235 0.0 11.716967 1 CGCGTAA 255 0.0 11.550445 10 TCGCGTA 255 0.0 11.550445 9 TTTGCGC 200 0.0 11.399548 17 GATATAC 500 0.0 11.393741 1 TCCAACG 210 0.0 11.308152 18 ATTTGCG 185 0.0 11.297541 16 CGTTATT 245 0.0 11.24193 2 TGCGCGC 195 0.0 11.203085 19 GCGTAAC 255 0.0 11.178078 11 AACCGCG 145 1.2150849E-9 11.13976 7 GTATCGA 60 0.005871904 11.084701 9 GTGCTAT 215 0.0 11.040445 1 >>END_MODULE