FastQCFastQC Report
Thu 2 Feb 2017
SRR4062143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456769
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24910.10139333409042525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33550.027.0937231
GGTATCA13650.026.4437071
GATATAC6300.020.262761
GTAGGAC13250.020.0898783
GTCCTAC12850.019.35471
TGTAGGA14600.018.9871962
TACACCG1658.367351E-1118.6660275
TCAACGC49000.018.5422234
GTATAGG3100.018.4596211
ATCAACG49300.018.429393
CAACGCA50050.018.1971785
TATCAAC50500.018.1236272
TTAGGAC7000.017.9136733
AACGCAG50900.017.8500776
TAGGACG8650.017.8028584
GCGAGAA9300.017.74132720
GTATTAG3850.017.7219751
ATACCGT6750.017.59946
GTAATAC3150.017.459723
TCCTACA15550.017.402752
Produced by FastQC (version 0.11.5)