##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062143_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2456769 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30717173653689 32.0 32.0 32.0 32.0 32.0 2 31.462215617341315 32.0 32.0 32.0 32.0 32.0 3 31.590997769835095 32.0 32.0 32.0 32.0 32.0 4 31.6642745003702 32.0 32.0 32.0 32.0 32.0 5 31.641120512347722 32.0 32.0 32.0 32.0 32.0 6 35.29336213539002 36.0 36.0 36.0 36.0 36.0 7 35.29977014525989 36.0 36.0 36.0 36.0 36.0 8 35.25225774177385 36.0 36.0 36.0 36.0 36.0 9 35.33258926663435 36.0 36.0 36.0 36.0 36.0 10 35.22067683205055 36.0 36.0 36.0 36.0 36.0 11 35.33514302728502 36.0 36.0 36.0 36.0 36.0 12 35.25992065188058 36.0 36.0 36.0 36.0 36.0 13 35.292277784358234 36.0 36.0 36.0 36.0 36.0 14 35.25789115704407 36.0 36.0 36.0 36.0 36.0 15 35.234878818480695 36.0 36.0 36.0 36.0 36.0 16 35.24551758834469 36.0 36.0 36.0 36.0 36.0 17 35.22715078218587 36.0 36.0 36.0 36.0 36.0 18 35.22912003529839 36.0 36.0 36.0 36.0 36.0 19 35.215665778915316 36.0 36.0 36.0 36.0 36.0 20 35.21582126768939 36.0 36.0 36.0 36.0 36.0 21 35.20435783746864 36.0 36.0 36.0 36.0 36.0 22 35.18701107023086 36.0 36.0 36.0 36.0 36.0 23 35.151857582051875 36.0 36.0 36.0 36.0 36.0 24 35.14122451072934 36.0 36.0 36.0 36.0 36.0 25 35.122906549211585 36.0 36.0 36.0 36.0 36.0 26 35.081991021540894 36.0 36.0 36.0 36.0 36.0 27 35.07085647857002 36.0 36.0 36.0 36.0 36.0 28 35.045336781764995 36.0 36.0 36.0 36.0 36.0 29 35.02660201264344 36.0 36.0 36.0 36.0 36.0 30 34.997729538267535 36.0 36.0 36.0 36.0 36.0 31 35.00000936188954 36.0 36.0 36.0 36.0 36.0 32 34.97637018376575 36.0 36.0 36.0 36.0 36.0 33 34.96468613858283 36.0 36.0 36.0 36.0 36.0 34 34.95650995270618 36.0 36.0 36.0 36.0 36.0 35 34.93149457682021 36.0 36.0 36.0 36.0 36.0 36 34.90595493512007 36.0 36.0 36.0 32.0 36.0 37 34.88537872303013 36.0 36.0 36.0 32.0 36.0 38 34.86992142932445 36.0 36.0 36.0 32.0 36.0 39 34.834756137023874 36.0 36.0 36.0 32.0 36.0 40 34.8353593683411 36.0 36.0 36.0 32.0 36.0 41 34.81168640600724 36.0 36.0 36.0 32.0 36.0 42 34.78430165799064 36.0 36.0 36.0 32.0 36.0 43 34.779923957034626 36.0 36.0 36.0 32.0 36.0 44 34.72811241105696 36.0 36.0 36.0 32.0 36.0 45 34.70870114365657 36.0 36.0 36.0 32.0 36.0 46 34.67454856358086 36.0 36.0 36.0 32.0 36.0 47 34.65580117626036 36.0 36.0 36.0 32.0 36.0 48 34.628108299966335 36.0 36.0 36.0 32.0 36.0 49 34.610161964759406 36.0 36.0 36.0 32.0 36.0 50 34.209158451608594 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 2.0 19 8.0 20 23.0 21 68.0 22 149.0 23 410.0 24 982.0 25 2498.0 26 5203.0 27 10181.0 28 17861.0 29 28810.0 30 44489.0 31 65806.0 32 101267.0 33 174019.0 34 400910.0 35 1604082.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83969146441617 17.827889294292444 12.387514764927692 25.944904476363696 2 15.84235031111321 19.69668599099103 38.02431917781119 26.436644520084567 3 19.043114473441058 23.308159276315735 28.175704443491068 29.473021806752143 4 12.607086787565294 16.002969754177133 35.43182122535737 35.9581222329002 5 14.606281024029567 36.730660034639044 33.04455249077547 15.618506450555913 6 34.84502193329689 35.16922850233661 16.417959049433666 13.567790514932836 7 30.735978840501488 29.937409662853938 19.901993227690514 19.42461826895406 8 27.907182156726982 33.785675413520764 18.692559210898544 19.614583218853706 9 27.10230387960773 14.447390047660157 18.522742675440792 39.92756339729132 10 16.507657008045935 26.733689654989952 30.7998025048346 25.958850832129514 11 37.25368563344783 21.20577880948514 22.107980033938883 19.43255552312814 12 24.88353605894571 23.31541956122045 28.66284945796695 23.13819492186689 13 29.660541955715008 19.620078240974223 24.977399177537652 25.74198062577312 14 23.628798637560145 19.733682735332465 24.888379819185282 31.74913880792211 15 25.07000047623525 27.514023499970897 22.01920489879187 25.396771125001983 16 25.582526544160896 25.986189099035112 23.95643847270078 24.474845884103217 17 23.963791467573873 26.247604068595788 25.00499639974291 24.783608064087424 18 24.564050692679192 25.282312309073113 26.338172352667023 23.815464645580672 19 25.697979744941424 24.846576947201793 25.128898972593678 24.326544335263105 20 25.88836371747551 24.64599774337299 24.92657007347877 24.53906846567273 21 26.635970510850026 24.207332560502255 24.519246424570817 24.637450504076902 22 25.984575676427045 24.589450615829165 25.002147129013757 24.423826578730033 23 24.518066011931122 24.608021595852765 25.55597435329669 25.317938038919426 24 24.923150190903392 24.86433240798782 25.464314498074685 24.7482029030341 25 24.961331247120196 24.617260754390735 25.424745722421854 24.996662276067216 26 24.2514926187216 25.661563460107555 25.853035466281554 24.233908454889296 27 24.95977427189702 24.89387449572832 25.334658112856722 24.811693119517937 28 24.553337558477338 25.063489097866626 25.472326731444067 24.91084661221197 29 24.432698174547706 24.83237075157087 25.49279315326155 25.24213792061987 30 24.458867322412377 25.088725122061916 25.681826970239076 24.770580585286627 31 25.25712727331933 24.766725280450675 24.917126622055065 25.059020824174926 32 24.975455398021452 25.02592854305196 24.814429783714434 25.18418627521215 33 24.526318146017225 24.85276369534734 25.39795218508893 25.22296597354651 34 25.15526635639175 25.099338718776785 25.455948641007254 24.289446283824212 35 25.35114266118249 24.822113633172076 25.61417148160284 24.212572224042592 36 24.22769184930227 25.32474695320135 25.379575285976365 25.06798591152001 37 25.214493832004862 24.991472348836698 25.001241495754083 24.792792323404353 38 24.717301463833188 25.011468314684855 25.16129925117095 25.109930970311005 39 25.585152434941566 24.475720595436336 24.866765197803765 25.07236177181833 40 25.796982051215256 24.872963177638262 25.123332768906142 24.20672200224034 41 24.729203156552344 25.099369853679544 25.760248069487464 24.411178920280648 42 25.630360706800197 25.414863906076935 25.09300270409 23.861772683032864 43 25.003195413584212 24.687236174849776 25.467568383885997 24.842000027680015 44 24.501546618991966 25.3052837422033 25.048144850973824 25.145024787830906 45 24.75512913038328 25.40355916038691 25.039046233915947 24.802265475313867 46 24.672416649468822 25.02414821816068 25.002981638399106 25.300453493971393 47 25.043360394995794 24.916525388371152 25.372984876268706 24.667129340364347 48 25.40616319472712 25.375267437581616 24.45258742807272 24.765981939618538 49 24.941221294499226 25.354911034114448 24.66165609409803 25.0422115772883 50 24.984053961313506 25.823261311918355 24.499424640059136 24.693260086709003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 122.5 2 67.0 3 175.5 4 284.0 5 320.0 6 356.0 7 286.0 8 216.0 9 327.0 10 438.0 11 697.5 12 957.0 13 1733.5 14 2510.0 15 3501.0 16 4492.0 17 4509.5 18 4527.0 19 4517.5 20 4508.0 21 5459.5 22 6411.0 23 7951.5 24 9492.0 25 12263.0 26 15034.0 27 19911.5 28 24789.0 29 29857.0 30 34925.0 31 39410.5 32 43896.0 33 50730.0 34 57564.0 35 66818.0 36 76072.0 37 86156.5 38 96241.0 39 101603.5 40 106966.0 41 114198.0 42 121430.0 43 125376.5 44 129323.0 45 140837.0 46 152351.0 47 163178.0 48 174005.0 49 181309.0 50 188613.0 51 184739.5 52 180866.0 53 180856.0 54 180846.0 55 181673.0 56 182500.0 57 176264.5 58 170029.0 59 156133.5 60 142238.0 61 124759.0 62 107280.0 63 91751.0 64 76222.0 65 64153.5 66 52085.0 67 44970.0 68 37855.0 69 32901.5 70 27948.0 71 21495.5 72 15043.0 73 13298.5 74 11554.0 75 8532.0 76 5510.0 77 4774.0 78 4038.0 79 3500.0 80 2962.0 81 2363.0 82 1764.0 83 1486.5 84 1209.0 85 961.0 86 713.0 87 483.0 88 253.0 89 157.0 90 61.0 91 47.5 92 34.0 93 33.5 94 33.0 95 28.5 96 24.0 97 33.0 98 42.0 99 28.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03174901669631943 2 0.004355313828854076 3 2.849270729156872E-4 4 0.0 5 1.6281547023753556E-4 6 8.140773511876778E-5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 5.698541458313744E-4 17 0.0 18 1.6281547023753556E-4 19 0.0 20 2.0351933779691947E-4 21 4.070386755938389E-5 22 0.0 23 4.070386755938389E-5 24 0.001180412159222133 25 2.0351933779691947E-4 26 5.291502782719906E-4 27 3.256309404750711E-4 28 0.001953785642850427 29 3.256309404750711E-4 30 9.768928214252135E-4 31 3.66334808034455E-4 32 6.919657485095262E-4 33 1.6281547023753556E-4 34 9.361889538658294E-4 35 1.6281547023753556E-4 36 4.0703867559383893E-4 37 0.0022387127157661143 38 0.0 39 6.512618809501422E-4 40 4.070386755938389E-5 41 0.0015467469672565876 42 0.0048844641071260665 43 0.005006575709804219 44 0.0048844641071260665 45 0.0026864552589193364 46 0.0026864552589193364 47 0.0013839314970190524 48 0.004925167974685451 49 0.003948275153260237 50 0.0018723779077316589 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2456769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.30116438162458 #Duplication Level Percentage of deduplicated Percentage of total 1 70.20752015298386 33.91104971735494 2 16.25504131998126 15.702748456530294 3 5.8517171997406425 8.479342631383581 4 2.7072797994856965 5.23059066488041 5 1.397638960655772 3.375379459239869 6 0.8428320471046519 2.442586155198177 7 0.5687030238772164 1.922831276843428 8 0.3689664806635954 1.4257208507073464 9 0.27531441233498094 1.1968206018140057 >10 1.3602702942197584 11.606128686362416 >50 0.08947529380910609 3.0037686752291197 >100 0.06354436527175564 6.2960617454271 >500 0.007882524913593796 2.6260489264601645 >1k 0.003814124958189748 2.7809221525693006 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2491 0.10139333409042525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.140773511876778E-5 2 0.0 0.0 0.0 0.0 8.140773511876778E-5 3 0.0 0.0 0.0 0.0 8.140773511876778E-5 4 0.0 0.0 0.0 0.0 8.140773511876778E-5 5 0.0 0.0 0.0 0.0 1.2211160267815166E-4 6 0.0 0.0 0.0 0.0 1.6281547023753556E-4 7 0.0 0.0 0.0 0.0 1.6281547023753556E-4 8 0.0 0.0 0.0 0.0 1.6281547023753556E-4 9 0.0 0.0 0.0 0.0 1.6281547023753556E-4 10 0.0 0.0 0.0 0.0 2.0351933779691944E-4 11 0.0 0.0 0.0 0.0 2.0351933779691944E-4 12 0.0 0.0 0.0 0.0 5.291502782719906E-4 13 0.0 0.0 0.0 0.0 6.919657485095262E-4 14 0.0 0.0 0.0 4.070386755938389E-5 6.919657485095262E-4 15 0.0 0.0 0.0 8.140773511876778E-5 8.954850863064456E-4 16 0.0 0.0 0.0 1.2211160267815166E-4 9.361889538658294E-4 17 0.0 0.0 0.0 1.6281547023753556E-4 9.361889538658294E-4 18 0.0 0.0 0.0 1.6281547023753556E-4 9.768928214252133E-4 19 0.0 0.0 0.0 1.6281547023753556E-4 9.768928214252133E-4 20 0.0 0.0 0.0 2.0351933779691944E-4 0.0010175966889845972 21 0.0 0.0 0.0 2.0351933779691944E-4 0.0010583005565439811 22 0.0 0.0 0.0 2.442232053563033E-4 0.001099004424103365 23 0.0 0.0 0.0 3.66334808034455E-4 0.001099004424103365 24 0.0 0.0 0.0 4.884464107126066E-4 0.001139708291662749 25 0.0 0.0 0.0 6.919657485095262E-4 0.001139708291662749 26 0.0 0.0 0.0 8.140773511876778E-4 0.0013025237619002845 27 0.0 0.0 0.0 9.768928214252133E-4 0.0013432276294596684 28 0.0 0.0 0.0 0.0012211160267815166 0.001424635364578436 29 0.0 0.0 0.0 0.0017502663050535073 0.001424635364578436 30 0.0 0.0 0.0 0.0025643436562411853 0.001424635364578436 31 0.0 0.0 0.0 0.0046809447693291475 0.0015467469672565879 32 0.0 0.0 0.0 0.006797545882417109 0.0015467469672565879 33 0.0 4.070386755938389E-5 0.0 0.009117666333301992 0.0016281547023753555 34 0.0 4.070386755938389E-5 0.0 0.011274971313949337 0.0016281547023753555 35 0.0 4.070386755938389E-5 0.0 0.014449872983581281 0.0016281547023753555 36 0.0 4.070386755938389E-5 0.0 0.01860166747463844 0.0016281547023753555 37 0.0 4.070386755938389E-5 0.0 0.024666543740986636 0.0016281547023753555 38 0.0 4.070386755938389E-5 0.0 0.032359574709710195 0.0016281547023753555 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3355 0.0 27.093723 1 GGTATCA 1365 0.0 26.443707 1 GATATAC 630 0.0 20.26276 1 GTAGGAC 1325 0.0 20.089878 3 GTCCTAC 1285 0.0 19.3547 1 TGTAGGA 1460 0.0 18.987196 2 TACACCG 165 8.367351E-11 18.666027 5 TCAACGC 4900 0.0 18.542223 4 GTATAGG 310 0.0 18.459621 1 ATCAACG 4930 0.0 18.42939 3 CAACGCA 5005 0.0 18.197178 5 TATCAAC 5050 0.0 18.123627 2 TTAGGAC 700 0.0 17.913673 3 AACGCAG 5090 0.0 17.850077 6 TAGGACG 865 0.0 17.802858 4 GCGAGAA 930 0.0 17.741327 20 GTATTAG 385 0.0 17.721975 1 ATACCGT 675 0.0 17.5994 6 GTAATAC 315 0.0 17.45972 3 TCCTACA 1555 0.0 17.40275 2 >>END_MODULE