FastQCFastQC Report
Thu 2 Feb 2017
SRR4062142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841244
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15650.18603401629016075No Hit
TATCAACGCAGAGTACTTTTTTTTT10590.12588499888260718No Hit
GTCCTACAGTGGACATTTCTAAATT9150.10876749195239431No Hit
CTGTAGGACGTGGAATATGGCAAGA8950.10639006043430918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC350.002165589516.2896447
ACGGTAT704.4168337E-714.9321749
TGTCGAG400.005280489614.24834918
GTCTTAA551.9663399E-413.8116461
CGGACCA707.234985E-613.5747039
TCGCGTA500.00149582513.3032089
GGTCTAG500.001503899113.293711
ACCATAC1301.4551915E-1113.1476261
GTTCAAA1750.012.479811
CCCGTTT853.9318475E-612.2956212
TGTAGGA5500.012.2600252
TAAACTG701.08850705E-412.2157795
CGCGTAA550.003060891312.09382610
TCTTACC550.003073199712.0873492
TAGAACA3250.011.98465254
GGTATCA4950.011.8933641
GACGTGA2650.011.8330437
CGAGCCG1052.715915E-711.76124115
GCGTAAC658.0044725E-411.69443211
ATTTGCG658.024489E-411.69095216
Produced by FastQC (version 0.11.5)