##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062142_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841244
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256684148713095	32.0	32.0	32.0	32.0	32.0
2	30.79988445682822	32.0	32.0	32.0	32.0	32.0
3	30.837871057624184	32.0	32.0	32.0	32.0	32.0
4	30.830530737812097	32.0	32.0	32.0	32.0	32.0
5	30.758435127026168	32.0	32.0	32.0	32.0	32.0
6	34.42426573027564	36.0	36.0	36.0	32.0	36.0
7	34.359659028771674	36.0	36.0	36.0	32.0	36.0
8	34.29137919557227	36.0	36.0	36.0	32.0	36.0
9	34.43003100170699	36.0	36.0	36.0	32.0	36.0
10	34.13209722743937	36.0	36.0	36.0	32.0	36.0
11	34.42978256011335	36.0	36.0	36.0	32.0	36.0
12	34.24522730622744	36.0	36.0	36.0	32.0	36.0
13	34.33359524703891	36.0	36.0	36.0	32.0	36.0
14	34.23913751539387	36.0	36.0	36.0	32.0	36.0
15	34.19257789654369	36.0	36.0	36.0	32.0	36.0
16	34.186674734084285	36.0	36.0	36.0	32.0	36.0
17	34.133148052170355	36.0	36.0	36.0	32.0	36.0
18	34.123329260000666	36.0	36.0	36.0	32.0	36.0
19	34.120010365601416	36.0	36.0	36.0	32.0	36.0
20	34.10166016042908	36.0	36.0	36.0	32.0	36.0
21	34.06315052469914	36.0	36.0	36.0	32.0	36.0
22	34.041949779136615	36.0	36.0	36.0	32.0	36.0
23	33.998065959460035	36.0	36.0	36.0	32.0	36.0
24	33.9757192919058	36.0	36.0	36.0	32.0	36.0
25	33.617731597491336	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	16.0
5	77.0
6	176.0
7	39.0
8	125.0
9	134.0
10	80.0
11	15.0
12	29.0
13	33.0
14	138.0
15	212.0
16	309.0
17	445.0
18	566.0
19	761.0
20	1237.0
21	1658.0
22	2411.0
23	3631.0
24	5128.0
25	7030.0
26	9751.0
27	12529.0
28	16651.0
29	21739.0
30	28368.0
31	38927.0
32	55310.0
33	77855.0
34	172642.0
35	383222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.0927086032728	17.71317986110946	11.833274293362878	25.36083724225487
2	16.08332421984585	19.252202690301505	39.37228675627998	25.292186333572666
3	19.92251904631145	22.802437832768096	28.148362118256525	29.12668100266393
4	12.69348740994365	15.464250897591365	35.85621894096108	35.98604275150391
5	14.413954826848938	36.92347391377764	33.870944726271254	14.791626533102164
6	34.377401221337	35.434949650179966	16.559536015643772	13.628113112839262
7	30.297401308508636	30.265152687107456	19.83492513797888	19.60252086640503
8	27.41576750844616	35.03462359116673	18.73827087917384	18.811338021213274
9	26.767566769851843	14.820937425988143	18.735666246547105	39.675829557612914
10	16.384007960786462	27.32056687274883	31.543783135226246	24.751642031238468
11	36.93219152644059	21.471135807406984	22.250473768915047	19.34619889723738
12	24.311186230866284	23.854357607065488	29.342490537291404	22.49196562477682
13	29.882284326794895	20.106408303180345	24.862645210436106	25.148662159588646
14	22.66285041028723	20.405348312734674	25.344147382075892	31.5876538949022
15	24.58404256585447	28.58209044053755	22.095558914904	24.73830807870398
16	24.710410465568806	26.519296249528406	24.822521157620255	23.94777212728253
17	23.169484888483158	26.859972839379804	25.833772719644553	24.13676955249248
18	23.493276210877067	26.143401166250147	27.225990717600858	23.137331905271928
19	25.437640406655753	25.259633324448842	25.260823211361995	24.04190305753341
20	25.172586114581474	25.52906181212446	25.62865131774645	23.669700755547623
21	25.624010946516744	24.940329585341185	24.856802903206614	24.57885656493545
22	25.205600080919144	25.77857903599117	25.34720858694941	23.668612296140275
23	23.982225606310266	25.884942328397987	25.807729600789976	24.325102464501775
24	24.429389179008137	25.56329339087332	25.85491975218303	24.152397677935507
25	24.304387244462106	25.502271315745013	26.02946880480486	24.163872634988017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	387.0
1	387.0
2	348.0
3	309.0
4	309.0
5	309.0
6	641.5
7	974.0
8	974.0
9	974.0
10	1202.0
11	1430.0
12	1430.0
13	1430.0
14	1900.0
15	2370.0
16	2370.0
17	2370.0
18	3954.0
19	5538.0
20	5538.0
21	5538.0
22	9140.5
23	12743.0
24	12743.0
25	12743.0
26	19576.5
27	26410.0
28	26410.0
29	26410.0
30	34619.0
31	42828.0
32	42828.0
33	42828.0
34	50074.5
35	57321.0
36	57321.0
37	57321.0
38	65668.0
39	74015.0
40	74015.0
41	74015.0
42	83743.0
43	93471.0
44	93471.0
45	93471.0
46	101777.0
47	110083.0
48	110083.0
49	110083.0
50	110834.5
51	111586.0
52	111586.0
53	111586.0
54	104206.5
55	96827.0
56	96827.0
57	96827.0
58	87097.5
59	77368.0
60	77368.0
61	77368.0
62	67049.5
63	56731.0
64	56731.0
65	56731.0
66	45840.5
67	34950.0
68	34950.0
69	34950.0
70	26421.0
71	17892.0
72	17892.0
73	17892.0
74	13601.0
75	9310.0
76	9310.0
77	9310.0
78	7204.5
79	5099.0
80	5099.0
81	5099.0
82	3547.0
83	1995.0
84	1995.0
85	1995.0
86	1445.0
87	895.0
88	895.0
89	895.0
90	599.5
91	304.0
92	304.0
93	304.0
94	193.0
95	82.0
96	82.0
97	82.0
98	204.0
99	326.0
100	326.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.132294554255365E-4
2	0.0
3	5.943578795212804E-4
4	0.012362643894042633
5	0.035899215923085336
6	0.0625264489256387
7	0.10674667516202196
8	0.10948072140781985
9	0.11970367693558585
10	0.13384939446819236
11	0.13931748695978813
12	0.13123421979829872
13	0.12885678828021357
14	0.1163752728102667
15	0.1348003670754264
16	0.11922819063196885
17	0.12564725573079866
18	0.11221476765361774
19	0.09842566484872402
20	0.09438403126797933
21	0.09438403126797933
22	0.10686554673792623
23	0.08427994731611756
24	0.09331418708484102
25	0.09058014083904313
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	841244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.30053903290087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18362009303276	46.54213896120513
2	13.982296961368194	16.862800873771878
3	4.271652417501638	7.7274883000962875
4	1.6866366235896664	4.068203902203553
5	0.8376454109017989	2.5255234897907113
6	0.4709756305011888	1.704005063434922
7	0.30929347589665196	1.3055394321149352
8	0.212911438894671	1.0270939625295357
9	0.1478872973884783	0.802591537377974
>10	0.784106137004688	8.661723841340601
>50	0.06540816473217421	2.7012273650987453
>100	0.0451880317481339	4.950047658769361
>500	0.0019819312000213547	0.8080173846065908
>1k	3.9638624000427094E-4	0.3135982276597684
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1565	0.18603401629016075	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1059	0.12588499888260718	No Hit
GTCCTACAGTGGACATTTCTAAATT	915	0.10876749195239431	No Hit
CTGTAGGACGTGGAATATGGCAAGA	895	0.10639006043430918	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1887157590425608E-4
2	0.0	0.0	0.0	0.0	1.1887157590425608E-4
3	0.0	0.0	0.0	0.0	1.1887157590425608E-4
4	0.0	0.0	0.0	0.0	1.1887157590425608E-4
5	0.0	0.0	0.0	0.0	1.1887157590425608E-4
6	0.0	0.0	0.0	0.0	1.1887157590425608E-4
7	0.0	0.0	0.0	0.0	1.1887157590425608E-4
8	0.0	0.0	0.0	0.0	1.1887157590425608E-4
9	0.0	0.0	0.0	0.0	1.1887157590425608E-4
10	0.0	0.0	0.0	0.0	1.1887157590425608E-4
11	0.0	0.0	0.0	0.0	1.1887157590425608E-4
12	0.0	0.0	0.0	0.0	3.5661472771276824E-4
13	0.0	0.0	0.0	0.0	3.5661472771276824E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAGTC	35	0.0021655895	16.289644	7
ACGGTAT	70	4.4168337E-7	14.932174	9
TGTCGAG	40	0.0052804896	14.248349	18
GTCTTAA	55	1.9663399E-4	13.811646	1
CGGACCA	70	7.234985E-6	13.574703	9
TCGCGTA	50	0.001495825	13.303208	9
GGTCTAG	50	0.0015038991	13.29371	1
ACCATAC	130	1.4551915E-11	13.147626	1
GTTCAAA	175	0.0	12.47981	1
CCCGTTT	85	3.9318475E-6	12.29562	12
TGTAGGA	550	0.0	12.260025	2
TAAACTG	70	1.08850705E-4	12.215779	5
CGCGTAA	55	0.0030608913	12.093826	10
TCTTACC	55	0.0030731997	12.087349	2
TAGAACA	325	0.0	11.9846525	4
GGTATCA	495	0.0	11.893364	1
GACGTGA	265	0.0	11.833043	7
CGAGCCG	105	2.715915E-7	11.761241	15
GCGTAAC	65	8.0044725E-4	11.694432	11
ATTTGCG	65	8.024489E-4	11.690952	16
>>END_MODULE