FastQCFastQC Report
Thu 2 Feb 2017
SRR4062142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841244
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAT1107.2759576E-1225.99873541
ATACGAA1151.2732926E-1124.86835540
GTATCAA11350.024.8182181
GGTATCA5650.023.3700311
CTAGCGG1306.730261E-1122.00154529
CTACACG803.614903E-621.998934
TGTAGGA6950.021.20762
ACTATAC1259.949872E-1021.1189733
GTAGGAC6150.020.7469583
CGAAATT755.4721484E-520.53233513
TCTAGCG1401.8371793E-1020.43000628
CAATACG1401.8553692E-1020.42757838
TCGCGTA1401.8553692E-1020.4275789
CGAATGC1452.9467628E-1019.72435243
CGCAATA1452.9467628E-1019.72317736
CGCGTAA1452.9467628E-1019.72317710
TAGGACA2350.019.6586194
GTCCTAA3700.019.6276661
CTGTAGG7350.019.4617771
TAGGACC4900.019.3051824
Produced by FastQC (version 0.11.5)