##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841244
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259292191088434	32.0	32.0	32.0	32.0	32.0
2	31.464894846203954	32.0	32.0	32.0	32.0	32.0
3	31.590501685598948	32.0	32.0	32.0	32.0	32.0
4	31.668364945247752	32.0	32.0	32.0	32.0	32.0
5	31.648796306422394	32.0	32.0	32.0	32.0	32.0
6	35.3031153862613	36.0	36.0	36.0	36.0	36.0
7	35.309289575913766	36.0	36.0	36.0	36.0	36.0
8	35.26649818601975	36.0	36.0	36.0	36.0	36.0
9	35.33991208258246	36.0	36.0	36.0	36.0	36.0
10	35.23021263747498	36.0	36.0	36.0	36.0	36.0
11	35.34089039565215	36.0	36.0	36.0	36.0	36.0
12	35.274234348179604	36.0	36.0	36.0	36.0	36.0
13	35.297306132346854	36.0	36.0	36.0	36.0	36.0
14	35.27029969901717	36.0	36.0	36.0	36.0	36.0
15	35.24178003052622	36.0	36.0	36.0	36.0	36.0
16	35.264127886796224	36.0	36.0	36.0	36.0	36.0
17	35.24479699112267	36.0	36.0	36.0	36.0	36.0
18	35.242540808612006	36.0	36.0	36.0	36.0	36.0
19	35.222949584187226	36.0	36.0	36.0	36.0	36.0
20	35.21862503625583	36.0	36.0	36.0	36.0	36.0
21	35.20782436486917	36.0	36.0	36.0	36.0	36.0
22	35.17991926242565	36.0	36.0	36.0	36.0	36.0
23	35.15966948947035	36.0	36.0	36.0	36.0	36.0
24	35.14110412674563	36.0	36.0	36.0	36.0	36.0
25	35.114400815934495	36.0	36.0	36.0	36.0	36.0
26	35.08175273761239	36.0	36.0	36.0	36.0	36.0
27	35.06726585865694	36.0	36.0	36.0	36.0	36.0
28	35.03529772574901	36.0	36.0	36.0	36.0	36.0
29	35.016754948623706	36.0	36.0	36.0	36.0	36.0
30	34.98487834682922	36.0	36.0	36.0	36.0	36.0
31	34.980411153006735	36.0	36.0	36.0	36.0	36.0
32	34.94076391629539	36.0	36.0	36.0	36.0	36.0
33	34.93469314491396	36.0	36.0	36.0	36.0	36.0
34	34.92448564269106	36.0	36.0	36.0	36.0	36.0
35	34.90585965546263	36.0	36.0	36.0	36.0	36.0
36	34.875353642938315	36.0	36.0	36.0	32.0	36.0
37	34.84098073804984	36.0	36.0	36.0	32.0	36.0
38	34.81912976496712	36.0	36.0	36.0	32.0	36.0
39	34.761758776288445	36.0	36.0	36.0	32.0	36.0
40	34.78082577706349	36.0	36.0	36.0	32.0	36.0
41	34.75759113883725	36.0	36.0	36.0	32.0	36.0
42	34.728566266148704	36.0	36.0	36.0	32.0	36.0
43	34.72732405818051	36.0	36.0	36.0	32.0	36.0
44	34.66655571986249	36.0	36.0	36.0	32.0	36.0
45	34.637729362705706	36.0	36.0	36.0	32.0	36.0
46	34.593765899073276	36.0	36.0	36.0	32.0	36.0
47	34.570191288139945	36.0	36.0	36.0	32.0	36.0
48	34.54309926727561	36.0	36.0	36.0	32.0	36.0
49	34.511270214111484	36.0	36.0	36.0	32.0	36.0
50	34.12578871290612	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	11.0
21	26.0
22	45.0
23	147.0
24	417.0
25	923.0
26	2053.0
27	3906.0
28	6676.0
29	10434.0
30	15680.0
31	23254.0
32	34666.0
33	59585.0
34	135565.0
35	547851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7642319249351	18.16834903130756	12.310057682772102	25.757361360985232
2	15.644688853701563	19.56367717055867	38.9908845808191	25.800749394920665
3	19.16808718537591	23.063636860736864	28.691149514646202	29.077126439241024
4	12.440029289956303	15.869949741097708	36.05826609164523	35.63175487730076
5	14.08261346602587	37.01582063684334	33.83029635907817	15.07126953805262
6	34.47038363560502	35.614170017866456	16.429893455026562	13.485552891501959
7	30.30832909358046	30.260780463218754	20.001212490074224	19.42967795312656
8	27.398115172292464	35.12631293655586	18.62705707262102	18.848514818530653
9	26.792226749908473	14.673626201197273	18.692555310944268	39.84159173794999
10	16.316331904099055	27.309707183298997	31.598242125045918	24.775718787556034
11	37.103860473299065	21.253286799073752	22.419773573422216	19.223079154204964
12	24.450485769272372	23.69779005590537	29.38496962233267	22.46675455248959
13	30.015429530552375	19.999310544859757	24.985022181436065	25.00023774315181
14	22.692940454850195	20.240382100793585	25.358397801351334	31.708279643004882
15	24.755481168364945	28.48305604557061	22.144229260476152	24.617233525588293
16	24.854767788939885	26.36349479755456	24.838601158529265	23.94313625497629
17	23.281235884000363	26.78057733546985	25.85765842014921	24.08052836038058
18	23.686525399350007	25.992639454520816	27.299397795164772	23.02143735096441
19	25.482499726595375	24.990371402351755	25.51471392366543	24.01241494738744
20	25.25937901193476	25.380985212305646	25.770410346631163	23.58922542912843
21	25.725829842471388	24.74216755186367	25.057177228009948	24.474825377654994
22	25.174146858699736	25.558696406750002	25.531712559019738	23.735444175530525
23	23.981270594500526	25.644165069825164	25.976529996053465	24.398034339620846
24	24.590929785542006	25.31842988361187	26.082780476845407	24.007859854000717
25	24.35161344773561	25.26252849952808	26.25546513369518	24.13039291904113
26	23.64643103802843	26.171278316863578	26.408785137160784	23.773505507947206
27	24.034553630758296	25.879442681841034	26.02886443037267	24.05713925702799
28	23.7242299138055	25.93561624132082	25.790353966836932	24.549799878036747
29	24.085102740947313	25.236852178089066	25.787466626725724	24.8905784542379
30	24.022153117555597	25.797160368790685	26.06379184913627	24.11689466451745
31	24.84350519826637	25.203568057705155	25.578252155741154	24.374674588287316
32	24.292765152145538	25.89576740835487	25.559119103602562	24.25234833589703
33	23.77832273985695	25.663157168992	26.280578336053523	24.27794175509753
34	24.243699426912638	25.74352171158288	26.31744118454697	23.695337676957514
35	24.365017004599146	25.730496420178234	26.287648158736538	23.61683841648608
36	23.64994098005209	25.79189554479632	26.207115440165186	24.35104803498641
37	24.357991362616747	25.67559053620485	25.524620583980308	24.441797517198093
38	24.188226008149837	25.925771833142345	25.686126736119363	24.199875422588452
39	25.029004940349676	25.142528374915006	25.66925333675687	24.15921334797845
40	25.4300773616216	25.254028557707397	25.712397354394206	23.6034967262768
41	24.455296188826694	25.36443356628394	26.535328331968277	23.64494191292109
42	24.708722802537775	26.2758719890063	25.80274343236936	23.21266177608656
43	24.089851630213182	25.722145150610608	26.234146329865705	23.953856889310504
44	23.7088180540795	26.029296034502845	25.921474644736247	24.340411266681407
45	23.95372446274586	26.182505925903033	25.733635116532856	24.130134494818254
46	24.071086807928914	25.342803649439805	25.679574430146513	24.90653511248477
47	24.758450660694503	25.52529848936565	25.77077062798073	23.945480221959116
48	24.511365608602723	25.91539450941606	25.173497602297612	24.399742279683604
49	24.551960248210932	25.90939349991679	25.204346068804835	24.33430018306745
50	24.2686339353854	26.47372788070858	25.10419217482888	24.153446009077143
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	109.0
2	24.0
3	74.5
4	125.0
5	138.5
6	152.0
7	116.0
8	80.0
9	125.5
10	171.0
11	292.0
12	413.0
13	773.5
14	1134.0
15	1483.5
16	1833.0
17	1891.5
18	1950.0
19	1942.5
20	1935.0
21	2232.0
22	2529.0
23	3043.5
24	3558.0
25	4596.0
26	5634.0
27	7806.5
28	9979.0
29	11864.5
30	13750.0
31	16118.5
32	18487.0
33	21707.5
34	24928.0
35	28872.0
36	32816.0
37	35486.5
38	38157.0
39	39701.5
40	41246.0
41	42791.0
42	44336.0
43	45591.0
44	46846.0
45	50292.0
46	53738.0
47	56615.0
48	59492.0
49	61385.0
50	63278.0
51	62812.0
52	62346.0
53	60695.0
54	59044.0
55	57955.5
56	56867.0
57	54288.0
58	51709.0
59	46942.0
60	42175.0
61	36752.0
62	31329.0
63	26831.5
64	22334.0
65	18970.0
66	15606.0
67	13205.5
68	10805.0
69	9469.0
70	8133.0
71	6499.5
72	4866.0
73	4283.0
74	3700.0
75	2850.0
76	2000.0
77	1730.0
78	1460.0
79	1210.0
80	960.0
81	742.0
82	524.0
83	416.0
84	308.0
85	237.0
86	166.0
87	114.0
88	62.0
89	41.0
90	20.0
91	18.0
92	16.0
93	14.0
94	12.0
95	10.5
96	9.0
97	7.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03150096761462786
2	0.003803890428936194
3	2.3774315180851214E-4
4	0.0
5	1.1887157590425607E-4
6	3.5661472771276824E-4
7	0.0
8	0.0
9	0.0
10	1.1887157590425607E-4
11	0.0
12	1.1887157590425607E-4
13	0.0
14	0.0
15	0.0
16	5.943578795212804E-4
17	0.0
18	2.3774315180851214E-4
19	0.0
20	4.7548630361702427E-4
21	0.0
22	0.0
23	0.0
24	8.321010313297925E-4
25	2.3774315180851214E-4
26	5.943578795212804E-4
27	1.1887157590425607E-4
28	8.321010313297925E-4
29	2.3774315180851214E-4
30	9.509726072340485E-4
31	2.3774315180851214E-4
32	0.0011887157590425607
33	3.5661472771276824E-4
34	0.0013075873349468168
35	1.1887157590425607E-4
36	3.5661472771276824E-4
37	0.0020208167903723534
38	0.0
39	9.509726072340485E-4
40	0.0
41	8.321010313297925E-4
42	0.004160505156648963
43	0.004160505156648963
44	0.004517119884361731
45	0.0026151746698936337
46	0.0022585599421808657
47	9.509726072340485E-4
48	0.0026151746698936337
49	0.002852917821702146
50	0.0011887157590425607
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	841244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.16319714432406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23554164163797	48.78443743259429
2	14.184428496315965	17.918677069847462
3	4.251654198050024	8.0564421690278
4	1.7115060852601531	4.324167851079894
5	0.826011561713022	2.60867655579853
6	0.439199438556216	1.6644744433921623
7	0.27309790069463324	1.2074815578893305
8	0.18802790595891802	0.950115495417405
9	0.1321119123236076	0.7510149686888715
>10	0.6713162724352993	7.556902159863929
>50	0.05567319951646973	2.369050129942049
>100	0.031052696126287874	3.6706650544690254
>500	3.786914114090136E-4	0.137895111989352
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.1887157590425608E-4	0.0
16	0.0	0.0	0.0	1.1887157590425608E-4	0.0
17	0.0	0.0	0.0	1.1887157590425608E-4	0.0
18	0.0	0.0	0.0	3.5661472771276824E-4	0.0
19	0.0	0.0	0.0	4.754863036170243E-4	0.0
20	0.0	0.0	0.0	5.943578795212804E-4	0.0
21	0.0	0.0	0.0	7.132294554255365E-4	0.0
22	0.0	0.0	0.0	9.509726072340486E-4	0.0
23	0.0	0.0	0.0	0.0015453304867553291	0.0
24	0.0	0.0	0.0	0.0021396883662766096	0.0
25	0.0	0.0	0.0	0.002852917821702146	0.0
26	0.0	0.0	0.0	0.003447275701223426	0.0
27	0.0	0.0	0.0	0.004398248308457475	0.0
28	0.0	0.0	0.0	0.005705835643404292	0.0
29	0.0	0.0	0.0	0.007845524009680901	0.0
30	0.0	0.0	0.0	0.01093618498319156	0.0
31	0.0	0.0	0.0	0.016642020626595852	0.0
32	0.0	0.0	0.0	0.02365544360494696	0.0
33	0.0	0.0	0.0	0.02971789397606402	0.0
34	0.0	0.0	0.0	0.036136959074893844	0.0
35	0.0	0.0	0.0	0.04291263890143644	0.0
36	0.0	0.0	0.0	0.05242236497377693	0.0
37	0.0	0.0	0.0	0.06585485305095787	0.0
38	0.0	0.0	0.0	0.08178364422212818	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	110	7.2759576E-12	25.998735	41
ATACGAA	115	1.2732926E-11	24.868355	40
GTATCAA	1135	0.0	24.818218	1
GGTATCA	565	0.0	23.370031	1
CTAGCGG	130	6.730261E-11	22.001545	29
CTACACG	80	3.614903E-6	21.99893	4
TGTAGGA	695	0.0	21.2076	2
ACTATAC	125	9.949872E-10	21.118973	3
GTAGGAC	615	0.0	20.746958	3
CGAAATT	75	5.4721484E-5	20.532335	13
TCTAGCG	140	1.8371793E-10	20.430006	28
CAATACG	140	1.8553692E-10	20.427578	38
TCGCGTA	140	1.8553692E-10	20.427578	9
CGAATGC	145	2.9467628E-10	19.724352	43
CGCAATA	145	2.9467628E-10	19.723177	36
CGCGTAA	145	2.9467628E-10	19.723177	10
TAGGACA	235	0.0	19.658619	4
GTCCTAA	370	0.0	19.627666	1
CTGTAGG	735	0.0	19.461777	1
TAGGACC	490	0.0	19.305182	4
>>END_MODULE