FastQCFastQC Report
Thu 2 Feb 2017
SRR4062141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125067
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC54214.3344767204778245No Hit
CCCATGTACTCTGCGTTGATACCAC43883.508519433583599No Hit
GTACATGGAAGCAGTGGTATCAACG30292.4219018606027167No Hit
TCCATGTACTCTGCGTTGATACCAC27012.159642431656632No Hit
GTATCAACGCAGAGTACATGGGAAG19891.5903475737004964No Hit
CATGTACTCTGCGTTGATACCACTG18861.507991716439988No Hit
GAGTACATGGGAAGCAGTGGTATCA17621.4088448591554927No Hit
TATCAACGCAGAGTACATGGGAAGC14531.1617772873739676No Hit
GCGTTGATACCACTGCTTCCCATGT14121.128994858755707No Hit
GCTTCCCATGTACTCTGCGTTGATA13021.0410420014872028No Hit
GAGTACATGGAAGCAGTGGTATCAA11910.9522895727889851No Hit
ACGCAGAGTACATGGGAAGCAGTGG11080.8859251441227503No Hit
ACTCTGCGTTGATACCACTGCTTCC10940.8747311441067588No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10380.829955144042793No Hit
GGTATCAACGCAGAGTACATGGGAA10120.8091662868702375No Hit
CAGTGGTATCAACGCAGAGTACATG9310.74440100106343No Hit
GCTTCCATGTACTCTGCGTTGATAC8420.673239143818913No Hit
GTATCAACGCAGAGTACTTTTTTTT8170.6532498580760713No Hit
AAAAAGTACTCTGCGTTGATACCAC7170.5732927151047039No Hit
CATGGAAGCAGTGGTATCAACGCAG6870.5493055722132937No Hit
TATCAACGCAGAGTACTTTTTTTTT6780.5421094293458707No Hit
GTACTCTGCGTTGATACCACTGCTT6680.5341137150487338No Hit
GCGTTGATACCACTGCTTCCATGTA6680.5341137150487338No Hit
GTATCAACGCAGAGTACATGGAAGC5070.40538271486483246No Hit
GTGGTATCAACGCAGAGTACATGGG5050.4037835720054051No Hit
CTGCTTCCCATGTACTCTGCGTTGA4540.36300542909000777No Hit
GCAGTGGTATCAACGCAGAGTACAT4340.3470140004957343No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4310.34461528620659326No Hit
ATACCACTGCTTCCCATGTACTCTG4260.34061742905802495No Hit
TATCAACGCAGAGTACATGGAAGCA4110.3286238576123198No Hit
AAGCAGTGGTATCAACGCAGAGTAC3890.31103328615861897No Hit
AAAGTACTCTGCGTTGATACCACTG3830.30623585758033695No Hit
GGGAAGCAGTGGTATCAACGCAGAG3810.3046367147209096No Hit
ACGCAGAGTACATGGAAGCAGTGGT3800.30383714329119593No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3710.2966410004237729No Hit
GCAGAGTACATGGGAAGCAGTGGTA3500.2798500003997857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3480.2782508575403584No Hit
GGTATCAACGCAGAGTACTTTTTTT3390.27105471467293535No Hit
TACCACTGCTTCCCATGTACTCTGC3370.26945557181350793No Hit
CCATGTACTCTGCGTTGATACCACT3350.2678564289540806No Hit
GAAGCAGTGGTATCAACGCAGAGTA3340.2670568575243669No Hit
CCACTGCTTCCCATGTACTCTGCGT3200.2558628575083755No Hit
ATCAACGCAGAGTACATGGGAAGCA3180.25426371464894815No Hit
CATGGGAAGCAGTGGTATCAACGCA3130.25026585750037983No Hit
GTTGATACCACTGCTTCCCATGTAC3110.24866671464095244No Hit
GGTATCAACGCAGAGTACATGGAAG2980.2382722860546747No Hit
ACATGGGAAGCAGTGGTATCAACGC2800.22388000031982858No Hit
GATACCACTGCTTCCCATGTACTCT2520.20149200028784572No Hit
ATACCACTGCTTCCATGTACTCTGC2510.20069242885813204No Hit
ATGGGAAGCAGTGGTATCAACGCAG2460.19669457170956367No Hit
GTGGTATCAACGCAGAGTACATGGA2270.18150271454500388No Hit
CCCCATGTACTCTGCGTTGATACCA2230.1783044288261492No Hit
CTTCCCATGTACTCTGCGTTGATAC1990.159114714513021No Hit
GGAAGCAGTGGTATCAACGCAGAGT1890.15111900021588429No Hit
CCACTGCTTCCATGTACTCTGCGTT1730.1383258573404655No Hit
GTACATGGGAGTGGTATCAACGCAA1710.13672671448103818No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1640.13112971447304245No Hit
ACATGGAAGCAGTGGTATCAACGCA1620.12953057161361511No Hit
TGATACCACTGCTTCCCATGTACTC1610.12873100018390143No Hit
TGGAAGCAGTGGTATCAACGCAGAG1590.12713185732447407No Hit
CCCATGTACTCTGCGTTGATACCAA1510.1207352858867647No Hit
GTACATGGGGTGGTATCAACGCAAA1470.11753700016791001No Hit
GTTGATACCACTGCTTCCATGTACT1450.11593785730848265No Hit
GTACATGGGAAGCAGTGGTATAAAC1320.10554342872220489No Hit
CCCATGTACTCTGCGTTGATACAAC1270.10154557157363653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTG400.005244735614.24946
ATACCAA500.00148213613.30476119
GATACAA655.3566815E-513.15855218
GGGAGTG701.075941E-412.2137717
ATGGGGT550.00304236712.09045
TGATACA752.0457033E-411.3995217
AAAAGTA1451.1623342E-911.1374622
ATACAAC600.005809101311.087319
AAAAAGT1502.0354491E-910.7662131
AAAGTAC1605.8917067E-910.0933253
AAGTACT1701.4806574E-910.05844
AGTACTC1701.4806574E-910.05845
TCCATGT6950.09.7046281
ATGGAAG8150.09.5578795
CCATGTA18900.09.4996012
CATGGGG800.00446818689.4995994
TGGGAGT800.00446818689.4995996
GGGAAGC13450.09.4642857
ACATGGA8650.09.4446893
TGGAAGC7950.09.3203626
Produced by FastQC (version 0.11.5)