##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062141_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125067
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.795605555422295	32.0	32.0	32.0	32.0	32.0
2	30.71846290388352	32.0	32.0	32.0	32.0	32.0
3	30.748862609641233	32.0	32.0	32.0	32.0	32.0
4	30.65970239951386	32.0	32.0	32.0	32.0	32.0
5	30.86562402552232	32.0	32.0	32.0	32.0	32.0
6	34.3102656975861	36.0	36.0	36.0	32.0	36.0
7	34.15092710307275	36.0	36.0	36.0	32.0	36.0
8	34.08853654441219	36.0	36.0	36.0	32.0	36.0
9	33.935218722764596	36.0	36.0	36.0	32.0	36.0
10	33.993755347133934	36.0	36.0	36.0	32.0	36.0
11	34.246124077494464	36.0	36.0	36.0	32.0	36.0
12	34.051260524358945	36.0	36.0	36.0	32.0	36.0
13	33.96849688566928	36.0	36.0	36.0	32.0	36.0
14	33.908481054154976	36.0	36.0	36.0	32.0	36.0
15	33.732175553903105	36.0	36.0	36.0	32.0	36.0
16	33.8746112083923	36.0	36.0	36.0	32.0	36.0
17	33.700128731000184	36.0	36.0	36.0	27.0	36.0
18	33.68899869669857	36.0	36.0	36.0	27.0	36.0
19	33.69705038099578	36.0	36.0	36.0	27.0	36.0
20	33.6101609537288	36.0	36.0	36.0	27.0	36.0
21	33.544500147920715	36.0	36.0	36.0	27.0	36.0
22	33.25219282464599	36.0	36.0	36.0	21.0	36.0
23	33.181142907401636	36.0	36.0	36.0	21.0	36.0
24	33.183173818833104	36.0	36.0	36.0	21.0	36.0
25	32.38010026625729	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	10.0
6	25.0
7	3.0
8	14.0
9	15.0
10	9.0
11	3.0
12	5.0
13	9.0
14	73.0
15	104.0
16	136.0
17	175.0
18	229.0
19	270.0
20	406.0
21	534.0
22	716.0
23	927.0
24	1110.0
25	1324.0
26	1742.0
27	2170.0
28	2728.0
29	3459.0
30	4629.0
31	6457.0
32	8926.0
33	12485.0
34	24503.0
35	51866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.42199531436156	16.542862397351733	12.239431326611388	22.795710961675315
2	10.950930301358472	23.672111748103017	36.45166190921666	28.92529604132185
3	20.277455723024026	31.75788589933235	25.89773317874705	22.066925198896573
4	12.229818879692935	20.227100075966575	31.264643556834994	36.2784374875055
5	10.957457869774212	39.956330131409516	34.329635524558306	14.75657647425797
6	32.382613426351874	29.878473194498895	20.821165985295174	16.917747393854054
7	25.012205174994197	32.13522533554227	23.96296029516515	18.88960919429838
8	30.46749317688866	30.89888989379157	22.335785118013813	16.297831811305954
9	28.848154966781397	16.236292323701274	18.203794124709837	36.71175858480749
10	16.142409953005036	31.131962179861816	31.886123275717132	20.839504591416013
11	30.056364189524587	25.923524042849593	18.364798001633282	25.65531376599254
12	23.863045061921113	28.76550029219402	28.993651784785097	18.37780286109977
13	32.35192878813349	20.87525915964234	22.184065384276714	24.588746667947454
14	24.418902478068773	22.98216686943715	25.409009412819362	27.189921239674714
15	32.35268218096816	21.190070206617193	26.46237101435353	19.99487659806111
16	21.130232930441046	25.630352997678703	32.92403746097815	20.315376610902106
17	25.90612192622951	25.692398821721312	29.48978611680328	18.9116931352459
18	28.33302651694027	21.087890890754686	31.048751790873947	19.5303308014311
19	22.038238989683954	32.6544005250058	29.25546814351226	16.051892341797984
20	22.52392842280483	23.925221678030667	33.476583757482636	20.07426614168187
21	20.75701196334974	29.321009882767175	27.9262193414156	21.995758812467493
22	21.370496630975815	29.197676093532433	22.416414590035373	27.01541268545638
23	17.3424234422386	31.102415760456424	25.428299365452784	26.126861431852188
24	19.188200741054924	36.28688268764455	24.382787678961563	20.142128892338963
25	23.393563049149368	25.130835587279737	21.878751020277516	29.596850343293372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	402.0
1	402.0
2	342.5
3	283.0
4	283.0
5	283.0
6	787.5
7	1292.0
8	1292.0
9	1292.0
10	1040.5
11	789.0
12	789.0
13	789.0
14	634.5
15	480.0
16	480.0
17	480.0
18	788.5
19	1097.0
20	1097.0
21	1097.0
22	1537.0
23	1977.0
24	1977.0
25	1977.0
26	2695.0
27	3413.0
28	3413.0
29	3413.0
30	3951.5
31	4490.0
32	4490.0
33	4490.0
34	4939.0
35	5388.0
36	5388.0
37	5388.0
38	6446.5
39	7505.0
40	7505.0
41	7505.0
42	10247.0
43	12989.0
44	12989.0
45	12989.0
46	27121.0
47	41253.0
48	41253.0
49	41253.0
50	31787.0
51	22321.0
52	22321.0
53	22321.0
54	15615.5
55	8910.0
56	8910.0
57	8910.0
58	6995.5
59	5081.0
60	5081.0
61	5081.0
62	4256.5
63	3432.0
64	3432.0
65	3432.0
66	2717.5
67	2003.0
68	2003.0
69	2003.0
70	1517.0
71	1031.0
72	1031.0
73	1031.0
74	755.5
75	480.0
76	480.0
77	480.0
78	349.0
79	218.0
80	218.0
81	218.0
82	168.5
83	119.0
84	119.0
85	119.0
86	85.0
87	51.0
88	51.0
89	51.0
90	39.0
91	27.0
92	27.0
93	27.0
94	16.0
95	5.0
96	5.0
97	5.0
98	18.0
99	31.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0031982857188546936
2	0.0
3	0.0015991428594273468
4	0.009594857156564081
5	0.030383714329119592
6	0.05916828579881183
7	0.09594857156564082
8	0.09914685728449552
9	0.10954128587077326
10	0.12793142875418775
11	0.13192928590275613
12	0.11993571445705102
13	0.115138285878769
14	0.10474385729249124
15	0.11993571445705102
16	0.10954128587077326
17	0.11114042873020061
18	0.1023451430033502
19	0.09275028584678613
20	0.08875242869821776
21	0.08155628583079469
22	0.08395500011993572
23	0.07675885725251265
24	0.08795285726850409
25	0.08075671440108102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	125067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.975892921394134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.26311111111112	38.79760448399658
2	9.685333333333332	8.712130298160186
3	1.9484444444444442	2.6289908608985586
4	0.632888888888889	1.138589715912271
5	0.3128888888888889	0.7036228581480327
6	0.19377777777777777	0.5229197150327425
7	0.1351111111111111	0.4253720006076743
8	0.09777777777777777	0.35181142907401636
9	0.051555555555555556	0.20868814315526876
>10	0.48177777777777775	4.194551720277931
>50	0.05333333333333334	1.7006884310009833
>100	0.09955555555555555	10.386432871980618
>500	0.017777777777777778	5.612991436589987
>1k	0.024888888888888887	20.281129314687327
>5k	0.0017777777777777776	4.3344767204778245
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5421	4.3344767204778245	No Hit
CCCATGTACTCTGCGTTGATACCAC	4388	3.508519433583599	No Hit
GTACATGGAAGCAGTGGTATCAACG	3029	2.4219018606027167	No Hit
TCCATGTACTCTGCGTTGATACCAC	2701	2.159642431656632	No Hit
GTATCAACGCAGAGTACATGGGAAG	1989	1.5903475737004964	No Hit
CATGTACTCTGCGTTGATACCACTG	1886	1.507991716439988	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1762	1.4088448591554927	No Hit
TATCAACGCAGAGTACATGGGAAGC	1453	1.1617772873739676	No Hit
GCGTTGATACCACTGCTTCCCATGT	1412	1.128994858755707	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1302	1.0410420014872028	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1191	0.9522895727889851	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1108	0.8859251441227503	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1094	0.8747311441067588	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1038	0.829955144042793	No Hit
GGTATCAACGCAGAGTACATGGGAA	1012	0.8091662868702375	No Hit
CAGTGGTATCAACGCAGAGTACATG	931	0.74440100106343	No Hit
GCTTCCATGTACTCTGCGTTGATAC	842	0.673239143818913	No Hit
GTATCAACGCAGAGTACTTTTTTTT	817	0.6532498580760713	No Hit
AAAAAGTACTCTGCGTTGATACCAC	717	0.5732927151047039	No Hit
CATGGAAGCAGTGGTATCAACGCAG	687	0.5493055722132937	No Hit
TATCAACGCAGAGTACTTTTTTTTT	678	0.5421094293458707	No Hit
GTACTCTGCGTTGATACCACTGCTT	668	0.5341137150487338	No Hit
GCGTTGATACCACTGCTTCCATGTA	668	0.5341137150487338	No Hit
GTATCAACGCAGAGTACATGGAAGC	507	0.40538271486483246	No Hit
GTGGTATCAACGCAGAGTACATGGG	505	0.4037835720054051	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	454	0.36300542909000777	No Hit
GCAGTGGTATCAACGCAGAGTACAT	434	0.3470140004957343	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	431	0.34461528620659326	No Hit
ATACCACTGCTTCCCATGTACTCTG	426	0.34061742905802495	No Hit
TATCAACGCAGAGTACATGGAAGCA	411	0.3286238576123198	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	389	0.31103328615861897	No Hit
AAAGTACTCTGCGTTGATACCACTG	383	0.30623585758033695	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	381	0.3046367147209096	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	380	0.30383714329119593	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	371	0.2966410004237729	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	350	0.2798500003997857	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	348	0.2782508575403584	No Hit
GGTATCAACGCAGAGTACTTTTTTT	339	0.27105471467293535	No Hit
TACCACTGCTTCCCATGTACTCTGC	337	0.26945557181350793	No Hit
CCATGTACTCTGCGTTGATACCACT	335	0.2678564289540806	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	334	0.2670568575243669	No Hit
CCACTGCTTCCCATGTACTCTGCGT	320	0.2558628575083755	No Hit
ATCAACGCAGAGTACATGGGAAGCA	318	0.25426371464894815	No Hit
CATGGGAAGCAGTGGTATCAACGCA	313	0.25026585750037983	No Hit
GTTGATACCACTGCTTCCCATGTAC	311	0.24866671464095244	No Hit
GGTATCAACGCAGAGTACATGGAAG	298	0.2382722860546747	No Hit
ACATGGGAAGCAGTGGTATCAACGC	280	0.22388000031982858	No Hit
GATACCACTGCTTCCCATGTACTCT	252	0.20149200028784572	No Hit
ATACCACTGCTTCCATGTACTCTGC	251	0.20069242885813204	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	246	0.19669457170956367	No Hit
GTGGTATCAACGCAGAGTACATGGA	227	0.18150271454500388	No Hit
CCCCATGTACTCTGCGTTGATACCA	223	0.1783044288261492	No Hit
CTTCCCATGTACTCTGCGTTGATAC	199	0.159114714513021	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	189	0.15111900021588429	No Hit
CCACTGCTTCCATGTACTCTGCGTT	173	0.1383258573404655	No Hit
GTACATGGGAGTGGTATCAACGCAA	171	0.13672671448103818	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	164	0.13112971447304245	No Hit
ACATGGAAGCAGTGGTATCAACGCA	162	0.12953057161361511	No Hit
TGATACCACTGCTTCCCATGTACTC	161	0.12873100018390143	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	159	0.12713185732447407	No Hit
CCCATGTACTCTGCGTTGATACCAA	151	0.1207352858867647	No Hit
GTACATGGGGTGGTATCAACGCAAA	147	0.11753700016791001	No Hit
GTTGATACCACTGCTTCCATGTACT	145	0.11593785730848265	No Hit
GTACATGGGAAGCAGTGGTATAAAC	132	0.10554342872220489	No Hit
CCCATGTACTCTGCGTTGATACAAC	127	0.10154557157363653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.995714297136735E-4	0.0
8	0.0	0.0	0.0	7.995714297136735E-4	0.0
9	0.0	0.0	0.0	7.995714297136735E-4	0.0
10	0.0	0.0	0.0	7.995714297136735E-4	0.0
11	0.0	0.0	0.0	7.995714297136735E-4	0.0
12	0.0	0.0	0.0	7.995714297136735E-4	0.0
13	0.0	0.0	0.0	7.995714297136735E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGGTG	40	0.0052447356	14.2494	6
ATACCAA	50	0.001482136	13.304761	19
GATACAA	65	5.3566815E-5	13.158552	18
GGGAGTG	70	1.075941E-4	12.213771	7
ATGGGGT	55	0.003042367	12.0904	5
TGATACA	75	2.0457033E-4	11.39952	17
AAAAGTA	145	1.1623342E-9	11.137462	2
ATACAAC	60	0.0058091013	11.0873	19
AAAAAGT	150	2.0354491E-9	10.766213	1
AAAGTAC	160	5.8917067E-9	10.093325	3
AAGTACT	170	1.4806574E-9	10.0584	4
AGTACTC	170	1.4806574E-9	10.0584	5
TCCATGT	695	0.0	9.704628	1
ATGGAAG	815	0.0	9.557879	5
CCATGTA	1890	0.0	9.499601	2
CATGGGG	80	0.0044681868	9.499599	4
TGGGAGT	80	0.0044681868	9.499599	6
GGGAAGC	1345	0.0	9.464285	7
ACATGGA	865	0.0	9.444689	3
TGGAAGC	795	0.0	9.320362	6
>>END_MODULE