##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125067
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.767644542525208	32.0	32.0	32.0	32.0	32.0
2	31.4236209391766	32.0	32.0	32.0	32.0	32.0
3	31.57897766796997	32.0	32.0	32.0	32.0	32.0
4	31.67961972382803	32.0	32.0	32.0	32.0	32.0
5	31.677165039538806	32.0	32.0	32.0	32.0	32.0
6	35.285399026122	36.0	36.0	36.0	36.0	36.0
7	35.2770195175386	36.0	36.0	36.0	36.0	36.0
8	35.25065764750094	36.0	36.0	36.0	36.0	36.0
9	35.24196630605995	36.0	36.0	36.0	36.0	36.0
10	35.210327264586184	36.0	36.0	36.0	36.0	36.0
11	35.256318613223314	36.0	36.0	36.0	36.0	36.0
12	35.19940511885629	36.0	36.0	36.0	36.0	36.0
13	35.13831786162617	36.0	36.0	36.0	36.0	36.0
14	35.14499428306428	36.0	36.0	36.0	36.0	36.0
15	35.04556757577938	36.0	36.0	36.0	36.0	36.0
16	35.045367682921956	36.0	36.0	36.0	36.0	36.0
17	34.956159498508796	36.0	36.0	36.0	36.0	36.0
18	34.91666066988094	36.0	36.0	36.0	36.0	36.0
19	34.900845147001206	36.0	36.0	36.0	36.0	36.0
20	34.79761247971088	36.0	36.0	36.0	32.0	36.0
21	34.71527261387896	36.0	36.0	36.0	32.0	36.0
22	34.48502802497861	36.0	36.0	36.0	32.0	36.0
23	34.33210199333158	36.0	36.0	36.0	32.0	36.0
24	34.33388503761984	36.0	36.0	36.0	32.0	36.0
25	34.19056185884366	36.0	36.0	36.0	32.0	36.0
26	33.973925975677034	36.0	36.0	36.0	32.0	36.0
27	33.846090495494416	36.0	36.0	36.0	32.0	36.0
28	33.75204490393149	36.0	36.0	36.0	32.0	36.0
29	33.444169924920246	36.0	36.0	36.0	21.0	36.0
30	33.60461992372088	36.0	36.0	36.0	27.0	36.0
31	33.18843499884062	36.0	36.0	36.0	14.0	36.0
32	33.319932516171335	36.0	36.0	36.0	21.0	36.0
33	33.08221193440316	36.0	36.0	36.0	14.0	36.0
34	33.013025018590035	36.0	36.0	36.0	14.0	36.0
35	32.80999784115714	36.0	36.0	36.0	14.0	36.0
36	32.51421238216316	36.0	36.0	36.0	14.0	36.0
37	32.29825613471179	36.0	36.0	36.0	14.0	36.0
38	32.00677237000968	36.0	32.0	36.0	14.0	36.0
39	31.520017270742883	36.0	32.0	36.0	14.0	36.0
40	31.06213469580305	36.0	32.0	36.0	14.0	36.0
41	30.99932835999904	36.0	32.0	36.0	14.0	36.0
42	30.7053899110077	36.0	32.0	36.0	14.0	36.0
43	30.406374183437677	36.0	27.0	36.0	14.0	36.0
44	30.777983001111405	36.0	32.0	36.0	14.0	36.0
45	29.870997145529994	36.0	21.0	36.0	14.0	36.0
46	30.2021796317174	36.0	21.0	36.0	14.0	36.0
47	29.950842348501205	36.0	21.0	36.0	14.0	36.0
48	29.865416136950593	36.0	21.0	36.0	14.0	36.0
49	30.211566600302238	36.0	21.0	36.0	14.0	36.0
50	29.554007052220012	36.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	26.0
22	68.0
23	175.0
24	462.0
25	1017.0
26	2010.0
27	3386.0
28	5121.0
29	6946.0
30	8753.0
31	10846.0
32	13082.0
33	12195.0
34	14448.0
35	46524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.090065589505684	17.325227963525837	12.558790593505039	23.025915853463445
2	10.507672255939102	23.84836199934432	36.650114744004924	28.99385100071165
3	19.968496885669282	31.973262331390373	26.29630518042329	21.761935602517053
4	12.153485731647837	19.799787313999694	31.363988901948552	36.68273805240391
5	10.477584014967977	40.23443434319205	34.130506048757866	15.15747559308211
6	32.38130267218908	30.033742184126783	20.785825084355462	16.799130059328675
7	24.9106478927295	32.01084218858692	23.995138605707343	19.083371312976244
8	30.73632532962332	30.30695547186708	22.49994003214277	16.45677916636683
9	29.61292747087561	15.414138022020197	18.443714169205304	36.529220337898884
10	16.168933451669908	30.520441043600634	32.33946604619924	20.971159458530227
11	30.238192328911705	25.643055322347223	18.538863169341234	25.57988917939984
12	24.32776032046823	28.08094861154421	29.275508327536443	18.315782740451116
13	32.37784547482549	20.34909288621299	22.886133032694474	24.38692860626704
14	25.00339817857628	22.167318317381884	25.858140036940203	26.971143467101633
15	32.20114018885877	20.602557029432226	27.047902324354144	20.14840045735486
16	21.31131811458042	24.94622796146004	33.500979490664854	20.241474433294687
17	26.18116689454452	25.088152750125932	29.84960061407086	18.881079741258684
18	28.49352746927647	20.465830314951187	31.78536304540766	19.255279170364684
19	22.719022603884316	31.36718718766741	30.273373471819102	15.640416736629167
20	23.204362461720518	23.173179175961682	33.693140476704485	19.92931788561331
21	21.30697945900997	27.97380603996258	29.04922961292747	21.66998488809998
22	21.570038459385767	28.114530611592187	23.159586461656552	27.15584446736549
23	17.68971831098531	29.672895328104136	26.537775752196822	26.099610608713732
24	19.716631885309475	34.93327576419039	25.191297464559	20.158794885941138
25	24.100075160315352	22.953480562263127	22.52810516047527	30.418339116946253
26	22.34979930596645	23.6746997585275	28.73682695536757	25.238673980138486
27	25.454356464934797	25.80776703686824	24.39732303485332	24.340553463343646
28	22.44610759291243	24.996801637561568	28.92119234951705	23.635898420008957
29	24.134043369794664	23.289675686048746	31.740548838994435	20.83573210516216
30	23.75766589108238	27.043904467205575	32.174754331678216	17.023675310033823
31	26.784472074521247	22.82892895694239	25.465158117778756	24.921440850757605
32	16.417731437800843	26.964962499800105	31.425007595989317	25.192298466409735
33	20.108421885869173	28.76618132680883	28.432760040618227	22.69263674670377
34	16.723969743975182	27.633409559752454	30.55026945772632	25.092351238546044
35	19.130546027329352	30.57081404367259	31.20647333029496	19.092166598703095
36	24.754731463935332	26.94155932420223	28.292835040418336	20.010874171444108
37	23.374245392395952	28.809818894175027	28.45640267061128	19.359533042817734
38	24.887460321267802	28.108134040154475	25.7821807511174	21.22222488746032
39	23.67808739455483	24.97901091432455	27.236237156678527	24.10666453444209
40	21.675581888107974	29.847201899781716	26.56815946652594	21.909056745584365
41	19.21929845602757	29.984728185692468	25.9692804656704	24.82669289260956
42	26.262383956629165	29.907005381373892	26.771735393128154	17.058875268868793
43	25.50933921832022	24.326744706710166	24.413900083157422	25.75001599181219
44	22.865709830887937	28.278894974613202	28.870587294606803	19.984807899892058
45	29.39168119233045	23.689092159339868	26.42204915804455	20.49717749028513
46	19.27797545276456	27.624835085755407	33.398632710990285	19.698556750489747
47	25.838950322627074	26.36906618052724	29.744057185348655	18.047926311497037
48	23.016831247751167	30.77599648182945	27.04753528165354	19.15963698876584
49	20.867882587176062	29.306029761000456	32.76188800844375	17.064199643379737
50	20.781526110840137	32.06383982472833	25.709442441009735	21.445191623421795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	177.0
1	124.5
2	72.0
3	240.5
4	409.0
5	348.0
6	287.0
7	249.0
8	211.0
9	278.0
10	345.0
11	433.5
12	522.0
13	798.0
14	1074.0
15	1259.5
16	1445.0
17	1426.0
18	1407.0
19	1242.5
20	1078.0
21	1093.5
22	1109.0
23	1127.5
24	1146.0
25	1100.0
26	1054.0
27	1119.5
28	1185.0
29	1309.5
30	1434.0
31	1617.0
32	1800.0
33	2015.0
34	2230.0
35	2428.0
36	2626.0
37	2864.0
38	3102.0
39	3456.5
40	3811.0
41	4553.5
42	5296.0
43	6764.0
44	8232.0
45	10775.0
46	13318.0
47	17012.5
48	20707.0
49	20301.0
50	19895.0
51	15332.5
52	10770.0
53	7701.5
54	4633.0
55	4180.0
56	3727.0
57	3401.5
58	3076.0
59	2823.0
60	2570.0
61	2344.0
62	2118.0
63	1739.5
64	1361.0
65	1154.5
66	948.0
67	819.0
68	690.0
69	567.0
70	444.0
71	364.0
72	284.0
73	229.5
74	175.0
75	145.5
76	116.0
77	96.0
78	76.0
79	61.5
80	47.0
81	38.5
82	30.0
83	24.5
84	19.0
85	11.5
86	4.0
87	4.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03757985719654265
2	0.004797428578282041
3	0.0
4	0.0
5	0.0
6	7.995714297136734E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0015991428594273468
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	7.995714297136734E-4
26	7.995714297136734E-4
27	0.0
28	0.0023987142891410203
29	0.0023987142891410203
30	0.0
31	0.0015991428594273468
32	7.995714297136734E-4
33	0.0
34	7.995714297136734E-4
35	0.0
36	0.0
37	0.0015991428594273468
38	0.0
39	0.0015991428594273468
40	0.0
41	0.0
42	0.004797428578282041
43	0.0023987142891410203
44	0.0015991428594273468
45	7.995714297136734E-4
46	0.0015991428594273468
47	0.0
48	0.0015991428594273468
49	0.0031982857188546936
50	0.0031982857188546936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	125067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.130969800187096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.85442263709378	52.226406646037724
2	8.89181426519866	10.693468300990668
3	1.9108026168820809	3.4469524334956465
4	0.6542205201850966	1.5735565736765094
5	0.3337588426147545	1.0034621442906602
6	0.2486569863305143	0.8971191441387416
7	0.17020371256848038	0.7164160010234514
8	0.12898250093080155	0.6204674294578106
9	0.09440987181532898	0.5109261435870374
>10	0.5704483804052976	6.546890866495558
>50	0.0651561087176214	2.7345342896207634
>100	0.06116695920429764	7.96213229708876
>500	0.007978299026647519	3.3246180047494542
>1k	0.007978299026647519	7.7430497253472135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2519	2.0141204314487435	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2188	1.7494622882135176	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1453	1.1617772873739676	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1346	1.0762231443946046	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1166	0.9323002870461432	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1012	0.8091662868702375	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	871	0.6964267152806095	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	725	0.5796892865424133	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	718	0.5740922865344176	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	645	0.5157235721653194	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	641	0.5125252864464647	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	558	0.4461608577802298	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	493	0.39418871484884105	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	434	0.3470140004957343	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	389	0.31103328615861897	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	387	0.3094341432991916	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	360	0.28784571469692244	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	353	0.28224871468892676	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	350	0.2798500003997857	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	349	0.27905042897007204	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	342	0.27345342896207636	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	319	0.25506328607866185	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	316	0.25266457178952084	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	314	0.2510654289300934	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	281	0.22467957174954226	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	258	0.20628942886612775	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	227	0.18150271454500388	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	220	0.17590571453700818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	219	0.1751061431072945	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	199	0.159114714513021	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	191	0.15271814307531165	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	190	0.15191857164559797	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	187	0.14951985735645695	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	185	0.1479207144970296	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	185	0.1479207144970296	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	180	0.14392285734846122	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	179	0.14312328591874754	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	175	0.13992500019989285	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	172	0.13752628591075183	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	167	0.13352842876218346	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	156	0.12473314303533306	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	152	0.12153485731647837	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	147	0.11753700016791001	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	145	0.11593785730848265	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTATGCGTTGATA	141	0.11273957158962795	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	140	0.11194000015991429	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	140	0.11194000015991429	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTATGCGTTGAT	137	0.10954128587077326	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	135	0.10794214301134593	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	133	0.10634300015191858	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	129	0.10314471443306387	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	127	0.10154557157363653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.995714297136735E-4	0.0
8	0.0	0.0	0.0	7.995714297136735E-4	0.0
9	0.0	0.0	0.0	7.995714297136735E-4	0.0
10	0.0	0.0	0.0	7.995714297136735E-4	0.0
11	0.0	0.0	0.0	7.995714297136735E-4	0.0
12	0.0	0.0	0.0	7.995714297136735E-4	7.995714297136735E-4
13	0.0	0.0	0.0	7.995714297136735E-4	7.995714297136735E-4
14	0.0	0.0	0.0	7.995714297136735E-4	7.995714297136735E-4
15	0.0	0.0	0.0	7.995714297136735E-4	7.995714297136735E-4
16	0.0	0.0	0.0	7.995714297136735E-4	7.995714297136735E-4
17	0.0	0.0	0.0	7.995714297136735E-4	7.995714297136735E-4
18	0.0	0.0	0.0	0.003198285718854694	7.995714297136735E-4
19	0.0	0.0	0.0	0.003198285718854694	7.995714297136735E-4
20	0.0	0.0	0.0	0.003198285718854694	7.995714297136735E-4
21	0.0	0.0	0.0	0.003997857148568367	7.995714297136735E-4
22	0.0	0.0	0.0	0.006396571437709388	7.995714297136735E-4
23	0.0	0.0	0.0	0.007196142867423061	7.995714297136735E-4
24	0.0	0.0	0.0	0.008795285726850408	7.995714297136735E-4
25	0.0	0.0	0.0	0.010394428586277754	7.995714297136735E-4
26	0.0	0.0	0.0	0.012793142875418776	7.995714297136735E-4
27	0.0	0.0	0.0	0.014392285734846123	7.995714297136735E-4
28	0.0	0.0	0.0	0.01839014288341449	7.995714297136735E-4
29	0.0	0.0	0.0	0.025586285750837552	7.995714297136735E-4
30	0.0	0.0	0.0	0.03678028576682898	7.995714297136735E-4
31	0.0	0.0	0.0	0.053571285790816124	7.995714297136735E-4
32	0.0	0.0	0.0	0.06876314295537592	7.995714297136735E-4
33	0.0	0.0	0.0	0.0935498572764998	7.995714297136735E-4
34	0.0	0.0	0.0	0.11753700016791	7.995714297136735E-4
35	0.0	0.0	0.0	0.14951985735645693	7.995714297136735E-4
36	0.0	0.0	0.0	0.18550057169357226	7.995714297136735E-4
37	0.0	0.0	0.0	0.22787785746839695	7.995714297136735E-4
38	0.0	0.0	0.0	0.31503114330718734	7.995714297136735E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCAGT	25	0.0023447617	35.2	40
CCCATGA	35	3.201517E-4	31.428572	31
AAAAAGT	180	0.0	30.555555	1
GGAATCT	30	0.00572559	29.333336	36
TCCTAGG	30	0.00572559	29.333336	2
AAAAGTA	200	0.0	28.599998	2
AGTGTTA	40	6.996698E-4	27.5	44
AGTGGTT	50	8.282873E-5	26.4	44
AGTACTC	245	0.0	23.34694	5
GCGATGA	95	2.636807E-8	23.157894	43
AAGTACT	240	0.0	22.916668	4
AAAGTAC	240	0.0	22.916668	3
TGCGATG	100	4.540925E-8	22.0	42
GGGAAGA	110	5.140464E-9	22.0	37
ATGCGAT	50	0.0025676426	22.0	41
CGATGAT	90	4.02164E-7	22.0	44
TGGGATG	90	4.02164E-7	22.0	36
TGGTATA	130	1.5825208E-9	20.307692	44
AGTGCTA	55	0.004461557	20.0	44
GCTTACC	55	0.004461557	20.0	27
>>END_MODULE