FastQCFastQC Report
Thu 2 Feb 2017
SRR4062140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93953
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC23342.4842208338211655No Hit
CCCATGTACTCTGCGTTGATACCAC16811.789192468574713No Hit
GTATCAACGCAGAGTACATGGGAAG11161.1878279565314571No Hit
GTATCAACGCAGAGTACTTTTTTTT10271.0930997413600418No Hit
GTACATGGAAGCAGTGGTATCAACG9911.0547827105041883No Hit
TCCATGTACTCTGCGTTGATACCAC8720.9281236362862282No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8630.9185443785722648No Hit
GAGTACATGGGAAGCAGTGGTATCA7400.7876278564814323No Hit
TATCAACGCAGAGTACTTTTTTTTT6880.7322810341340883No Hit
CATGTACTCTGCGTTGATACCACTG6810.7248305003565613No Hit
GCGTTGATACCACTGCTTCCCATGT6300.6705480399774355No Hit
TATCAACGCAGAGTACATGGGAAGC6180.6577756963588177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5890.6269091992804913No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4860.5172799165540216No Hit
ACGCAGAGTACATGGGAAGCAGTGG4790.5098293827764946No Hit
GGTATCAACGCAGAGTACATGGGAA4720.5023788489989676No Hit
GAGTACATGGAAGCAGTGGTATCAA4510.48002724766638644No Hit
GCTTCCCATGTACTCTGCGTTGATA4260.4534181984609326No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4240.4512894745244963No Hit
GGTATCAACGCAGAGTACTTTTTTT4100.43638840696944214No Hit
ACTCTGCGTTGATACCACTGCTTCC3760.400200100050025No Hit
CAGTGGTATCAACGCAGAGTACATG3450.3672048790352623No Hit
AAAAAGTACTCTGCGTTGATACCAC3340.3554968973848626No Hit
GCTTCCATGTACTCTGCGTTGATAC2720.28950645535533726No Hit
GTGGTATCAACGCAGAGTACATGGG2590.2756697497685013No Hit
GTACTCTGCGTTGATACCACTGCTT2410.25651123434057455No Hit
CATGGAAGCAGTGGTATCAACGCAG2380.2533181484359201No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2350.25012506253126565No Hit
GTACATGGGAGTGGTATCAACGCAA2080.22138728938937555No Hit
GCGTTGATACCACTGCTTCCATGTA2080.22138728938937555No Hit
GGGAAGCAGTGGTATCAACGCAGAG1960.20861494577075773No Hit
GCAGTGGTATCAACGCAGAGTACAT1800.19158515427926728No Hit
GTATCAACGCAGAGTACATGGGAGT1770.18839206837461284No Hit
AAAGTACTCTGCGTTGATACCACTG1740.1851989824699584No Hit
CTGCTTCCCATGTACTCTGCGTTGA1660.17668408672421315No Hit
ATACCACTGCTTCCCATGTACTCTG1640.17455536278777686No Hit
GTATCAACGCAGAGTACATGGAAGC1630.1734910008195587No Hit
ATCAACGCAGAGTACATGGGAAGCA1630.1734910008195587No Hit
GCAGAGTACATGGGAAGCAGTGGTA1530.1628473811373772No Hit
AAGCAGTGGTATCAACGCAGAGTAC1470.15646120932806828No Hit
CCATGTACTCTGCGTTGATACCACT1400.14901067555054123No Hit
CATGGGAAGCAGTGGTATCAACGCA1340.14262450374123234No Hit
GAAGCAGTGGTATCAACGCAGAGTA1320.140495779804796No Hit
GTTGATACCACTGCTTCCCATGTAC1290.13730269390014155No Hit
TACCACTGCTTCCCATGTACTCTGC1250.13304524602726894No Hit
ATCAACGCAGAGTACTTTTTTTTTT1250.13304524602726894No Hit
GTCCTAAAGTGTGTATTTCTCATTT1240.1319808840590508No Hit
CTTTAGGACGTGAAATATGGCGAGG1230.13091652209083265No Hit
CTGTAGGACGTGGAATATGGCAAGA1190.12665907421796005No Hit
GTCCTACAGTGGACATTTCTAAATT1180.1255947122497419No Hit
ACGCAGAGTACATGGAAGCAGTGGT1160.1234659883133056No Hit
GATACCACTGCTTCCCATGTACTCT1160.1234659883133056No Hit
CCCCATGTACTCTGCGTTGATACCA1130.12027290240865113No Hit
ACATGGGAAGCAGTGGTATCAACGC1090.11601545453577852No Hit
GGTATCAACGCAGAGTACATGGGAG980.10430747288537887No Hit
CCACTGCTTCCCATGTACTCTGCGT970.10324311091716072No Hit
GGTATCAACGCAGAGTACATGGAAG970.10324311091716072No Hit
TATCAACGCAGAGTACATGGAAGCA940.10005002501250625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGA307.631754E-419.0063977
CGTGAAA307.631754E-419.0063979
GGACGTG359.788248E-519.0063956
AGGACGT359.828437E-518.9962715
TTTAGGA307.6592E-418.996272
GTGAAAT350.002144995816.29119710
TGAAATA456.661896E-414.78275311
TAGGACG456.688918E-414.7748784
GTCCTAC653.2980552E-614.6125161
ACGTGAA400.00521866314.2547988
CTTTAGG400.005237175614.2472041
TTAGGAC400.005237175614.2472043
TCCTACA707.1047198E-613.5687652
CCTACAG604.0354242E-412.664183
TACAGTG701.07196334E-412.2118885
AAGTACT1353.4924597E-1011.9606144
AAAAAGT1353.4924597E-1011.9606141
AGTACTC1406.384653E-1011.533455
AAAAGTA1406.384653E-1011.533452
AAAGTAC1406.384653E-1011.533453
Produced by FastQC (version 0.11.5)