##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062140_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93953 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.71194107692144 32.0 32.0 32.0 32.0 32.0 2 30.62402477834662 32.0 32.0 32.0 32.0 32.0 3 30.662054431471056 32.0 32.0 32.0 32.0 32.0 4 30.54094068310751 32.0 32.0 32.0 32.0 32.0 5 30.680499824380274 32.0 32.0 32.0 32.0 32.0 6 34.16535927538237 36.0 36.0 36.0 32.0 36.0 7 34.0725149808947 36.0 36.0 36.0 32.0 36.0 8 33.9210349855779 36.0 36.0 36.0 32.0 36.0 9 33.82535948825477 36.0 36.0 36.0 32.0 36.0 10 33.8354922141922 36.0 36.0 36.0 32.0 36.0 11 34.05810351984503 36.0 36.0 36.0 32.0 36.0 12 33.92074760784648 36.0 36.0 36.0 32.0 36.0 13 33.85226655881132 36.0 36.0 36.0 32.0 36.0 14 33.778548848892534 36.0 36.0 36.0 32.0 36.0 15 33.67191042329676 36.0 36.0 36.0 27.0 36.0 16 33.79217268208572 36.0 36.0 36.0 32.0 36.0 17 33.63581790895448 36.0 36.0 36.0 27.0 36.0 18 33.590295147573784 36.0 36.0 36.0 27.0 36.0 19 33.613423733143165 36.0 36.0 36.0 27.0 36.0 20 33.45620682681766 36.0 36.0 36.0 21.0 36.0 21 33.361691484039895 36.0 36.0 36.0 21.0 36.0 22 33.242121060530266 36.0 36.0 36.0 21.0 36.0 23 33.17169222909327 36.0 36.0 36.0 21.0 36.0 24 33.077996445031026 36.0 36.0 36.0 14.0 36.0 25 32.58573967834981 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 7.0 6 22.0 7 5.0 8 23.0 9 13.0 10 10.0 11 2.0 12 10.0 13 4.0 14 96.0 15 128.0 16 151.0 17 209.0 18 223.0 19 277.0 20 398.0 21 469.0 22 636.0 23 776.0 24 907.0 25 1055.0 26 1382.0 27 1659.0 28 1963.0 29 2630.0 30 3454.0 31 4626.0 32 6325.0 33 8574.0 34 17678.0 35 40237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.27197155964279 16.900299092079916 11.508126576619727 23.319602771657564 2 12.554149415133098 22.42504230838824 38.8704990793269 26.150309197151767 3 20.266516236841824 30.33431609421732 26.059838429853226 23.33932923908763 4 12.13275994209316 18.425870731499614 33.09205484118198 36.34931448522524 5 11.68510375520373 39.686764690224976 32.84179593922681 15.786335615344486 6 31.804141280729407 32.82241915555366 19.31852072770653 16.054918836010394 7 26.108825848803257 32.742598947121635 22.223406295956863 18.925168908118245 8 28.816991165531718 32.49677632490382 20.75620490851157 17.93002760105289 9 29.57803523656246 15.435447598136918 18.55195421165386 36.43456295364676 10 16.45773371708773 29.957360622534914 30.7440571367658 22.840848523611555 11 31.3498704815103 24.84729610165336 20.93402551993945 22.868807896896886 12 25.85166709303317 26.775816491856403 29.209303317131408 18.16321309797902 13 31.249600306964254 21.579160538040117 23.727910298224298 23.443328856771334 14 24.383979195975613 22.92918958093533 25.533955748816982 27.15287547427207 15 28.058750173202164 25.073277837584335 26.170047217573888 20.697924771639613 16 20.695718898871377 27.293751532009676 30.794726689473627 21.21580287964532 17 23.209413369713506 26.512406207366983 29.094901091405184 21.183279331514324 18 24.677885178988202 23.7528374878774 31.748958255624355 19.820319077510046 19 22.98347133859778 28.43547854257915 30.02973240832507 18.55131771049799 20 23.879499584407835 25.0026640523433 31.21736535879457 19.900471004454296 21 22.360149174214172 26.853489611081514 28.48907831646244 22.297282898241875 22 22.722477381954583 28.173186560245522 25.336473396489804 23.767862661310087 23 19.06471754000895 29.595968205936877 27.815543290642914 23.523770963411256 24 20.52768424193342 31.925322876262737 27.439154341247175 20.10783854055667 25 22.413076603407674 25.95074962438863 26.729677027502213 24.90649674470148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 666.0 1 666.0 2 476.0 3 286.0 4 286.0 5 286.0 6 699.5 7 1113.0 8 1113.0 9 1113.0 10 970.0 11 827.0 12 827.0 13 827.0 14 627.0 15 427.0 16 427.0 17 427.0 18 699.5 19 972.0 20 972.0 21 972.0 22 1396.0 23 1820.0 24 1820.0 25 1820.0 26 2593.5 27 3367.0 28 3367.0 29 3367.0 30 4189.5 31 5012.0 32 5012.0 33 5012.0 34 5130.5 35 5249.0 36 5249.0 37 5249.0 38 5852.0 39 6455.0 40 6455.0 41 6455.0 42 8007.5 43 9560.0 44 9560.0 45 9560.0 46 15556.0 47 21552.0 48 21552.0 49 21552.0 50 17811.0 51 14070.0 52 14070.0 53 14070.0 54 11005.5 55 7941.0 56 7941.0 57 7941.0 58 6716.0 59 5491.0 60 5491.0 61 5491.0 62 4782.5 63 4074.0 64 4074.0 65 4074.0 66 3338.5 67 2603.0 68 2603.0 69 2603.0 70 1963.0 71 1323.0 72 1323.0 73 1323.0 74 982.5 75 642.0 76 642.0 77 642.0 78 453.0 79 264.0 80 264.0 81 264.0 82 190.0 83 116.0 84 116.0 85 116.0 86 84.0 87 52.0 88 52.0 89 52.0 90 35.5 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 26.0 99 48.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002128723936436303 2 0.0 3 0.0 4 0.009579257713963365 5 0.03193085904654455 6 0.07344097580705246 7 0.12240162634508744 8 0.1234659883133056 9 0.1383670558683597 10 0.15220376145519568 11 0.15326812342341384 12 0.14581758964588679 13 0.13943141783657786 14 0.13304524602726894 15 0.140495779804796 16 0.1298521601226145 17 0.13730269390014155 18 0.1277234361861782 19 0.1234659883133056 20 0.11814417847221483 21 0.10962928272646962 22 0.11920854044043297 23 0.10537183485359702 24 0.11601545453577852 25 0.11282236863112408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 93953.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91469138824732 #Duplication Level Percentage of deduplicated Percentage of total 1 83.26549598790557 45.7249901546518 2 11.468387797030664 12.595659531893608 3 2.6282125828584717 4.32982448671144 4 0.9070822188626584 1.99248560450438 5 0.41477691204403616 1.1388673059934222 6 0.25972012249486376 0.8557470224473939 7 0.162809629026631 0.6258448373122731 8 0.11435438229251463 0.5023788489989676 9 0.07946660464395085 0.39274956627249796 >10 0.53300771407528 5.891243494087469 >50 0.06202271581966896 2.427809649505604 >100 0.07946660464395085 9.866635445382265 >500 0.017443888824281894 7.1014230519515085 >1k 0.00775283947745862 6.554341000287378 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2334 2.4842208338211655 No Hit CCCATGTACTCTGCGTTGATACCAC 1681 1.789192468574713 No Hit GTATCAACGCAGAGTACATGGGAAG 1116 1.1878279565314571 No Hit GTATCAACGCAGAGTACTTTTTTTT 1027 1.0930997413600418 No Hit GTACATGGAAGCAGTGGTATCAACG 991 1.0547827105041883 No Hit TCCATGTACTCTGCGTTGATACCAC 872 0.9281236362862282 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 863 0.9185443785722648 No Hit GAGTACATGGGAAGCAGTGGTATCA 740 0.7876278564814323 No Hit TATCAACGCAGAGTACTTTTTTTTT 688 0.7322810341340883 No Hit CATGTACTCTGCGTTGATACCACTG 681 0.7248305003565613 No Hit GCGTTGATACCACTGCTTCCCATGT 630 0.6705480399774355 No Hit TATCAACGCAGAGTACATGGGAAGC 618 0.6577756963588177 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 589 0.6269091992804913 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 486 0.5172799165540216 No Hit ACGCAGAGTACATGGGAAGCAGTGG 479 0.5098293827764946 No Hit GGTATCAACGCAGAGTACATGGGAA 472 0.5023788489989676 No Hit GAGTACATGGAAGCAGTGGTATCAA 451 0.48002724766638644 No Hit GCTTCCCATGTACTCTGCGTTGATA 426 0.4534181984609326 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 424 0.4512894745244963 No Hit GGTATCAACGCAGAGTACTTTTTTT 410 0.43638840696944214 No Hit ACTCTGCGTTGATACCACTGCTTCC 376 0.400200100050025 No Hit CAGTGGTATCAACGCAGAGTACATG 345 0.3672048790352623 No Hit AAAAAGTACTCTGCGTTGATACCAC 334 0.3554968973848626 No Hit GCTTCCATGTACTCTGCGTTGATAC 272 0.28950645535533726 No Hit GTGGTATCAACGCAGAGTACATGGG 259 0.2756697497685013 No Hit GTACTCTGCGTTGATACCACTGCTT 241 0.25651123434057455 No Hit CATGGAAGCAGTGGTATCAACGCAG 238 0.2533181484359201 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 235 0.25012506253126565 No Hit GTACATGGGAGTGGTATCAACGCAA 208 0.22138728938937555 No Hit GCGTTGATACCACTGCTTCCATGTA 208 0.22138728938937555 No Hit GGGAAGCAGTGGTATCAACGCAGAG 196 0.20861494577075773 No Hit GCAGTGGTATCAACGCAGAGTACAT 180 0.19158515427926728 No Hit GTATCAACGCAGAGTACATGGGAGT 177 0.18839206837461284 No Hit AAAGTACTCTGCGTTGATACCACTG 174 0.1851989824699584 No Hit CTGCTTCCCATGTACTCTGCGTTGA 166 0.17668408672421315 No Hit ATACCACTGCTTCCCATGTACTCTG 164 0.17455536278777686 No Hit GTATCAACGCAGAGTACATGGAAGC 163 0.1734910008195587 No Hit ATCAACGCAGAGTACATGGGAAGCA 163 0.1734910008195587 No Hit GCAGAGTACATGGGAAGCAGTGGTA 153 0.1628473811373772 No Hit AAGCAGTGGTATCAACGCAGAGTAC 147 0.15646120932806828 No Hit CCATGTACTCTGCGTTGATACCACT 140 0.14901067555054123 No Hit CATGGGAAGCAGTGGTATCAACGCA 134 0.14262450374123234 No Hit GAAGCAGTGGTATCAACGCAGAGTA 132 0.140495779804796 No Hit GTTGATACCACTGCTTCCCATGTAC 129 0.13730269390014155 No Hit TACCACTGCTTCCCATGTACTCTGC 125 0.13304524602726894 No Hit ATCAACGCAGAGTACTTTTTTTTTT 125 0.13304524602726894 No Hit GTCCTAAAGTGTGTATTTCTCATTT 124 0.1319808840590508 No Hit CTTTAGGACGTGAAATATGGCGAGG 123 0.13091652209083265 No Hit CTGTAGGACGTGGAATATGGCAAGA 119 0.12665907421796005 No Hit GTCCTACAGTGGACATTTCTAAATT 118 0.1255947122497419 No Hit ACGCAGAGTACATGGAAGCAGTGGT 116 0.1234659883133056 No Hit GATACCACTGCTTCCCATGTACTCT 116 0.1234659883133056 No Hit CCCCATGTACTCTGCGTTGATACCA 113 0.12027290240865113 No Hit ACATGGGAAGCAGTGGTATCAACGC 109 0.11601545453577852 No Hit GGTATCAACGCAGAGTACATGGGAG 98 0.10430747288537887 No Hit CCACTGCTTCCCATGTACTCTGCGT 97 0.10324311091716072 No Hit GGTATCAACGCAGAGTACATGGAAG 97 0.10324311091716072 No Hit TATCAACGCAGAGTACATGGAAGCA 94 0.10005002501250625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGA 30 7.631754E-4 19.006397 7 CGTGAAA 30 7.631754E-4 19.006397 9 GGACGTG 35 9.788248E-5 19.006395 6 AGGACGT 35 9.828437E-5 18.996271 5 TTTAGGA 30 7.6592E-4 18.99627 2 GTGAAAT 35 0.0021449958 16.291197 10 TGAAATA 45 6.661896E-4 14.782753 11 TAGGACG 45 6.688918E-4 14.774878 4 GTCCTAC 65 3.2980552E-6 14.612516 1 ACGTGAA 40 0.005218663 14.254798 8 CTTTAGG 40 0.0052371756 14.247204 1 TTAGGAC 40 0.0052371756 14.247204 3 TCCTACA 70 7.1047198E-6 13.568765 2 CCTACAG 60 4.0354242E-4 12.66418 3 TACAGTG 70 1.07196334E-4 12.211888 5 AAGTACT 135 3.4924597E-10 11.960614 4 AAAAAGT 135 3.4924597E-10 11.960614 1 AGTACTC 140 6.384653E-10 11.53345 5 AAAAGTA 140 6.384653E-10 11.53345 2 AAAGTAC 140 6.384653E-10 11.53345 3 >>END_MODULE