FastQCFastQC Report
Thu 2 Feb 2017
SRR4062140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93953
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7130.7588900833395421No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.6630975061999085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5000.5321809841090758No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3810.40552190989111575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.36826924100348046No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3250.3459176396708993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.3395314678615904No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.33740274392515407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.28844209338711907No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2300.2448032526901749No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2220.23628835694442965No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC2190.23309527103977523No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1840.1958426021521399No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1570.1671048290102498No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1440.15326812342341384No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.14581758964588679No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.13091652209083265No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1170.12453035028152375No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1120.11920854044043297No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1000.10643619682181517No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.10643619682181517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG505.8256774E-835.214481
TTTCTCG300.005706929629.34540241
GTCCTAA655.81289E-727.0880641
TCCTAAA701.1149641E-625.1398142
CCTAAAG602.8478936E-421.9973413
CTAAAGT602.8478936E-421.9973414
AACGCAT500.0025647321.9973423
TAAAGTG550.004456553619.997585
CGTGATT550.004456553619.9975828
GACGTGG550.004456553619.997587
CCGTGAT550.004456553619.9975827
AAAGTAC1101.2364217E-719.997583
TGTAGGA909.86976E-619.553192
AAGTACT1252.224624E-819.3576584
CTGTAGG808.84863E-519.2579211
AAAAGTA1051.7591337E-618.8548622
TGTACAA951.5733573E-518.52407635
CGGCGGC600.007345136318.34087839
CATGGAC600.007367753418.33111833
CGTGGAA600.007367753418.3311189
Produced by FastQC (version 0.11.5)