##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93953
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.597511521718307	32.0	32.0	32.0	32.0	32.0
2	31.3419156386704	32.0	32.0	32.0	32.0	32.0
3	31.5270294721829	32.0	32.0	32.0	32.0	32.0
4	31.676582972337233	32.0	32.0	32.0	32.0	32.0
5	31.668547039477186	32.0	32.0	32.0	32.0	32.0
6	35.28400370397965	36.0	36.0	36.0	36.0	36.0
7	35.294264153353275	36.0	36.0	36.0	36.0	36.0
8	35.283343799559354	36.0	36.0	36.0	36.0	36.0
9	35.27023086011091	36.0	36.0	36.0	36.0	36.0
10	35.204549083052164	36.0	36.0	36.0	36.0	36.0
11	35.27424350473109	36.0	36.0	36.0	36.0	36.0
12	35.166838738518194	36.0	36.0	36.0	36.0	36.0
13	35.14975572892829	36.0	36.0	36.0	36.0	36.0
14	35.124530350281525	36.0	36.0	36.0	36.0	36.0
15	35.05464434344832	36.0	36.0	36.0	36.0	36.0
16	35.04555469223974	36.0	36.0	36.0	36.0	36.0
17	35.029536044618055	36.0	36.0	36.0	36.0	36.0
18	34.968792907091846	36.0	36.0	36.0	36.0	36.0
19	34.94780368907858	36.0	36.0	36.0	36.0	36.0
20	34.87480974529818	36.0	36.0	36.0	36.0	36.0
21	34.820186689089226	36.0	36.0	36.0	32.0	36.0
22	34.69056868859962	36.0	36.0	36.0	32.0	36.0
23	34.62904856683661	36.0	36.0	36.0	32.0	36.0
24	34.52528391855502	36.0	36.0	36.0	32.0	36.0
25	34.42684108011453	36.0	36.0	36.0	32.0	36.0
26	34.27147616361372	36.0	36.0	36.0	32.0	36.0
27	34.09652698689771	36.0	36.0	36.0	32.0	36.0
28	33.99761582919119	36.0	36.0	36.0	32.0	36.0
29	33.83639692186519	36.0	36.0	36.0	32.0	36.0
30	33.809500494928315	36.0	36.0	36.0	32.0	36.0
31	33.616670037146235	36.0	36.0	36.0	27.0	36.0
32	33.675263163496645	36.0	36.0	36.0	27.0	36.0
33	33.55457515992038	36.0	36.0	36.0	21.0	36.0
34	33.43697380605196	36.0	36.0	36.0	21.0	36.0
35	33.25699019722627	36.0	36.0	36.0	14.0	36.0
36	33.0634359733058	36.0	36.0	36.0	14.0	36.0
37	32.93340287164859	36.0	36.0	36.0	14.0	36.0
38	32.76030568475727	36.0	36.0	36.0	14.0	36.0
39	32.399774355262736	36.0	36.0	36.0	14.0	36.0
40	32.12001745553628	36.0	36.0	36.0	14.0	36.0
41	32.0660649473673	36.0	36.0	36.0	14.0	36.0
42	31.861026257809755	36.0	36.0	36.0	14.0	36.0
43	31.672357455323407	36.0	32.0	36.0	14.0	36.0
44	31.804327695762776	36.0	36.0	36.0	14.0	36.0
45	31.293167860526008	36.0	32.0	36.0	14.0	36.0
46	31.405745425904442	36.0	32.0	36.0	14.0	36.0
47	31.233020765702	36.0	32.0	36.0	14.0	36.0
48	31.1096718572052	36.0	32.0	36.0	14.0	36.0
49	31.194980468957883	36.0	32.0	36.0	14.0	36.0
50	30.743478122039743	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	1.0
20	9.0
21	19.0
22	47.0
23	115.0
24	323.0
25	872.0
26	1576.0
27	2609.0
28	3617.0
29	4150.0
30	4679.0
31	5574.0
32	6680.0
33	7052.0
34	12320.0
35	44309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.7059199318569	18.357112436115845	12.311541737649064	23.625425894378193
2	12.330093987163249	22.958200725925767	38.3005673290828	26.411137957828185
3	19.594903834896172	30.21510755377689	26.820857237129204	23.36913137419774
4	12.094345044862857	18.19633220865752	32.90368588549594	36.80563686098368
5	11.408895937330367	39.29943695251881	33.42096580204996	15.870701308100859
6	32.00962183219269	32.71210073121667	19.15957978989495	16.118697646695686
7	25.949144785158534	31.84251700318244	22.806083893010335	19.402254318648687
8	29.06772535203772	31.70627867125052	21.412834076612775	17.813161900098986
9	28.7675752770002	14.320990282375229	19.462922950837118	37.44851148978744
10	16.240034911072556	29.223122199397572	31.715857928964482	22.820984960565387
11	32.12244420082382	23.58093940587315	21.446893659595755	22.849722733707278
12	25.337136653433102	26.38553319212798	29.89367023937501	18.383659915063916
13	32.030909071557055	20.693325386097303	24.325992783625853	22.949772758719785
14	24.012006003001503	21.592711249241642	27.322171724159954	27.07311102359691
15	27.984204868391643	24.59314763764861	26.606920481517353	20.81572701244239
16	20.85639475476839	25.635430858310627	31.884366485013626	21.62380790190736
17	23.256309005566614	25.887411791001885	29.84790267474163	21.008376528689876
18	24.556959330729196	22.04932253360723	33.46247591881047	19.931242216853107
19	23.219056336678978	26.996476961885197	31.240088129170967	18.544378572264854
20	23.632280313351497	23.482203678474114	32.79440565395095	20.09111035422343
21	22.128085319255373	25.29136908879972	30.131022958287655	22.449522633657253
22	22.551701382606197	26.650559322214303	26.994348237948763	23.803391057230744
23	19.079752642278585	27.440315902632168	29.741466477919808	23.738464977169436
24	21.084395056997796	29.89111345275729	28.987450905259127	20.037040584985792
25	22.644300873841175	23.518142049748278	27.793684076080595	26.043873000329953
26	21.07649569465584	24.647430098027737	31.249667386884934	23.026406820431493
27	22.848658371739063	25.757559630879268	28.59089119027599	22.80289080710568
28	21.054144093323256	25.619192575010906	30.78986301661469	22.536800315051142
29	22.490207765667574	24.020776566757494	31.86627213896458	21.622743528610357
30	21.772820163487737	26.328337874659404	32.6954189373297	19.20342302452316
31	24.637850840313774	23.552201632731258	28.963417879152342	22.846529647802623
32	18.282740122615802	26.742379087193463	32.10256301089918	22.872317779291553
33	19.900375719774782	28.056581482230474	30.948452949879197	21.094589848115547
34	18.674429495912808	26.916936307901906	31.615080040871934	22.793554155313352
35	19.016955286153713	28.853788596425872	32.649303375091804	19.47995274232861
36	22.162380790190735	26.585916212534062	30.829572547683924	20.42213044959128
37	21.146968664850135	28.08987568119891	30.11218494550409	20.650970708446867
38	22.357987504390493	28.387598054346324	28.707971006779985	20.5464434344832
39	22.430602861035425	25.827018051771116	29.325613079019075	22.41676600817439
40	21.452215469436847	28.441880514725447	29.29337008929997	20.81253392653774
41	20.34847210839462	28.240716102732215	29.3540387214884	22.056773067384754
42	22.797138781853405	29.212526344921553	29.381772507610755	18.60856236561429
43	22.021522315298725	26.969951782349998	28.651715292339457	22.356810610011816
44	20.653539116551357	28.77488025545503	29.54550292708888	21.026077700904736
45	23.485396798365123	27.153227179836513	28.33468153950954	21.02669448228883
46	19.486546321525886	28.473050068119893	30.78486886920981	21.255534741144412
47	22.347343884708312	27.95759581918619	29.483890881611018	20.21116941449448
48	20.89005737155265	29.850237895028155	28.48566775591012	20.774036977509073
49	20.914935286103542	29.82373978201635	29.85141348773842	19.409911444141688
50	20.165616485013622	31.893945844686648	26.671066076294274	21.26937159400545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	341.0
1	210.5
2	80.0
3	315.0
4	550.0
5	485.0
6	420.0
7	322.0
8	224.0
9	300.0
10	376.0
11	474.5
12	573.0
13	922.5
14	1272.0
15	1486.5
16	1701.0
17	1562.0
18	1423.0
19	1228.0
20	1033.0
21	1015.0
22	997.0
23	991.0
24	985.0
25	913.5
26	842.0
27	952.0
28	1062.0
29	1241.0
30	1420.0
31	1734.5
32	2049.0
33	2514.0
34	2979.0
35	3216.5
36	3454.0
37	3505.5
38	3557.0
39	3719.0
40	3881.0
41	4222.5
42	4564.0
43	5188.5
44	5813.0
45	6513.0
46	7213.0
47	8128.0
48	9043.0
49	8847.0
50	8651.0
51	7408.5
52	6166.0
53	5296.0
54	4426.0
55	4277.0
56	4128.0
57	3900.5
58	3673.0
59	3399.0
60	3125.0
61	2784.0
62	2443.0
63	2091.5
64	1740.0
65	1503.5
66	1267.0
67	1089.0
68	911.0
69	747.5
70	584.0
71	459.5
72	335.0
73	299.0
74	263.0
75	211.0
76	159.0
77	124.0
78	89.0
79	76.5
80	64.0
81	51.5
82	39.0
83	33.0
84	27.0
85	16.5
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035123944951199
2	0.004257447872872606
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010643619682181515
17	0.0
18	0.0
19	0.0
20	0.0010643619682181515
21	0.0
22	0.0
23	0.0
24	0.002128723936436303
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0010643619682181515
30	0.0010643619682181515
31	0.0
32	0.0010643619682181515
33	0.0
34	0.0010643619682181515
35	0.0
36	0.0010643619682181515
37	0.0010643619682181515
38	0.0
39	0.0010643619682181515
40	0.0
41	0.0
42	0.007450533777527061
43	0.004257447872872606
44	0.0031930859046544547
45	0.0010643619682181515
46	0.0010643619682181515
47	0.0
48	0.004257447872872606
49	0.0010643619682181515
50	0.0010643619682181515
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	93953.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.72841740018946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77406478609903	61.52437921088204
2	9.918238340431214	14.22839079114025
3	2.2065260939888116	4.748118740221175
4	0.7820035316288524	2.243675029003864
5	0.37096941728123933	1.3304524602726897
6	0.20180736300099417	0.8685193660660118
7	0.13503286789037114	0.6779985737549626
8	0.0905165378166224	0.519408640490458
9	0.054903473757623424	0.3544325354166445
>10	0.3902598269798638	5.3526763381690845
>50	0.04748408541199864	2.361819207476078
>100	0.02522592037512427	4.368141517567294
>500	0.002967755338249915	1.4219875895394505
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	713	0.7588900833395421	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	623	0.6630975061999085	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	500	0.5321809841090758	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	381	0.40552190989111575	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	346	0.36826924100348046	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	325	0.3459176396708993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	319	0.3395314678615904	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	317	0.33740274392515407	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	271	0.28844209338711907	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	230	0.2448032526901749	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	222	0.23628835694442965	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	219	0.23309527103977523	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	184	0.1958426021521399	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	157	0.1671048290102498	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	144	0.15326812342341384	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.14581758964588679	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.13091652209083265	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	117	0.12453035028152375	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	112	0.11920854044043297	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT	100	0.10643619682181517	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100	0.10643619682181517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0010643619682181517
2	0.0	0.0	0.0	0.0	0.0010643619682181517
3	0.0	0.0	0.0	0.0	0.0010643619682181517
4	0.0	0.0	0.0	0.0	0.0010643619682181517
5	0.0	0.0	0.0	0.0	0.0010643619682181517
6	0.0	0.0	0.0	0.0	0.0010643619682181517
7	0.0	0.0	0.0	0.0	0.0010643619682181517
8	0.0	0.0	0.0	0.0	0.0010643619682181517
9	0.0	0.0	0.0	0.0	0.0010643619682181517
10	0.0	0.0	0.0	0.0	0.0010643619682181517
11	0.0	0.0	0.0	0.0	0.0010643619682181517
12	0.0	0.0	0.0	0.0	0.0010643619682181517
13	0.0	0.0	0.0	0.0	0.0010643619682181517
14	0.0	0.0	0.0	0.0	0.0010643619682181517
15	0.0	0.0	0.0	0.0	0.0010643619682181517
16	0.0	0.0	0.0	0.0021287239364363034	0.0010643619682181517
17	0.0	0.0	0.0	0.003193085904654455	0.0010643619682181517
18	0.0	0.0	0.0	0.003193085904654455	0.0010643619682181517
19	0.0	0.0	0.0	0.005321809841090758	0.0010643619682181517
20	0.0	0.0	0.0	0.005321809841090758	0.003193085904654455
21	0.0	0.0	0.0	0.008514895745745214	0.003193085904654455
22	0.0	0.0	0.0	0.01277234361861782	0.003193085904654455
23	0.0	0.0	0.0	0.024480325269017486	0.003193085904654455
24	0.0	0.0	0.0	0.03193085904654455	0.003193085904654455
25	0.0	0.0	0.0	0.035123944951199006	0.003193085904654455
26	0.0	0.0	0.0	0.043638840696944216	0.003193085904654455
27	0.0	0.0	0.0	0.05747554628378019	0.003193085904654455
28	0.0	0.0	0.0	0.07769842367992506	0.003193085904654455
29	0.0	0.0	0.0	0.09153512926676104	0.003193085904654455
30	0.0	0.0	0.0	0.12346598831330559	0.003193085904654455
31	0.0	0.0	0.0	0.16604046704203165	0.003193085904654455
32	0.0	0.0	0.0	0.21819420348472107	0.003193085904654455
33	0.0	0.0	0.0	0.27992719764137386	0.003193085904654455
34	0.0	0.0	0.0	0.3256947622747544	0.003193085904654455
35	0.0	0.0	0.0	0.40658627185933394	0.003193085904654455
36	0.0	0.0	0.0	0.4864134194756953	0.003193085904654455
37	0.0	0.0	0.0	0.5917852543292923	0.003193085904654455
38	0.0	0.0	0.0	0.7376028439751791	0.003193085904654455
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACATG	50	5.8256774E-8	35.21448	1
TTTCTCG	30	0.0057069296	29.345402	41
GTCCTAA	65	5.81289E-7	27.088064	1
TCCTAAA	70	1.1149641E-6	25.139814	2
CCTAAAG	60	2.8478936E-4	21.997341	3
CTAAAGT	60	2.8478936E-4	21.997341	4
AACGCAT	50	0.00256473	21.99734	23
TAAAGTG	55	0.0044565536	19.99758	5
CGTGATT	55	0.0044565536	19.99758	28
GACGTGG	55	0.0044565536	19.99758	7
CCGTGAT	55	0.0044565536	19.99758	27
AAAGTAC	110	1.2364217E-7	19.99758	3
TGTAGGA	90	9.86976E-6	19.55319	2
AAGTACT	125	2.224624E-8	19.357658	4
CTGTAGG	80	8.84863E-5	19.257921	1
AAAAGTA	105	1.7591337E-6	18.854862	2
TGTACAA	95	1.5733573E-5	18.524076	35
CGGCGGC	60	0.0073451363	18.340878	39
CATGGAC	60	0.0073677534	18.331118	33
CGTGGAA	60	0.0073677534	18.331118	9
>>END_MODULE