FastQCFastQC Report
Thu 2 Feb 2017
SRR4062139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541864
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26320.17070247440760014No Hit
TATCAACGCAGAGTACTTTTTTTTT16460.10675390306797486No Hit
GCGCAAGACGGACCAGAGCGAAAGC15430.10007367705582335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGTA456.7524693E-414.7806079
CGTCTTA655.449279E-513.154654515
GTATTAC655.4742377E-513.148251
GGTATCA7900.012.9818171
CGGTCCA2900.012.77789410
TCCAACG1001.4454963E-712.34795418
GAATATA701.09522996E-412.2090891
TAACGAA1103.8082362E-812.09204613
CGAACGA1103.8135113E-812.09086816
CGCGGTC951.0377462E-612.00190710
GTATCAA19650.011.888071
GCTAGAC802.8692797E-511.8738023
TCGCACG1459.640644E-1111.79421213
CGCATCG1950.011.69340613
ACGGTAT2300.011.5674319
CAAGACG3450.011.5648034
GTGTAGG1657.2759576E-1211.5102521
GCCGGTC3150.011.4613948
AACCGCG1001.9271683E-611.40107257
GCGTTAT1251.8428182E-811.395151
Produced by FastQC (version 0.11.5)