##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062139_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1541864
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25988024884166	32.0	32.0	32.0	32.0	32.0
2	30.768314844889044	32.0	32.0	32.0	32.0	32.0
3	30.78375070693654	32.0	32.0	32.0	32.0	32.0
4	30.788660348772655	32.0	32.0	32.0	32.0	32.0
5	30.680678710962834	32.0	32.0	32.0	32.0	32.0
6	34.37233698951399	36.0	36.0	36.0	32.0	36.0
7	34.30298716358901	36.0	36.0	36.0	32.0	36.0
8	34.2409375924206	36.0	36.0	36.0	32.0	36.0
9	34.40289805067113	36.0	36.0	36.0	32.0	36.0
10	34.03716605355596	36.0	36.0	36.0	32.0	36.0
11	34.40290194206493	36.0	36.0	36.0	32.0	36.0
12	34.1802817887959	36.0	36.0	36.0	32.0	36.0
13	34.28859808647196	36.0	36.0	36.0	32.0	36.0
14	34.16914656545584	36.0	36.0	36.0	32.0	36.0
15	34.116556324033766	36.0	36.0	36.0	32.0	36.0
16	34.12504345389736	36.0	36.0	36.0	32.0	36.0
17	34.05292295559141	36.0	36.0	36.0	32.0	36.0
18	34.06285508968366	36.0	36.0	36.0	32.0	36.0
19	34.06953336999891	36.0	36.0	36.0	32.0	36.0
20	34.04899264785999	36.0	36.0	36.0	32.0	36.0
21	34.03497260458769	36.0	36.0	36.0	32.0	36.0
22	34.01445004228648	36.0	36.0	36.0	32.0	36.0
23	33.969950008561064	36.0	36.0	36.0	32.0	36.0
24	33.94407094270312	36.0	36.0	36.0	32.0	36.0
25	33.575160973989924	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	16.0
5	116.0
6	325.0
7	78.0
8	253.0
9	200.0
10	89.0
11	39.0
12	78.0
13	77.0
14	195.0
15	310.0
16	513.0
17	640.0
18	982.0
19	1363.0
20	2152.0
21	3036.0
22	4585.0
23	6663.0
24	9625.0
25	13349.0
26	18531.0
27	23644.0
28	31483.0
29	41697.0
30	54827.0
31	74122.0
32	105413.0
33	148883.0
34	322906.0
35	675671.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.018422662111966	17.40155177121525	11.697498655836394	25.88252691083639
2	16.778825574306357	19.350459834226193	37.43854824692255	26.4321663445449
3	19.149982164283166	22.911567273081037	28.264876609268086	29.67357395336771
4	12.849829119092544	15.558302853247385	35.37238027471968	36.21948775294039
5	15.019041749116035	36.394783793427834	33.2925682031985	15.29360625425763
6	34.76027275022276	35.04367910379474	16.564377761784208	13.631670384198294
7	30.706019457817053	30.194480784026023	20.154390019834377	18.94510973832255
8	28.160225116106336	33.23650501271602	18.942424374246436	19.66084549693121
9	27.04676131696595	14.47013256020077	18.53979241001133	39.94331371282194
10	16.36368831762591	26.75149887259843	31.064394372343852	25.820418437431808
11	37.17203665577731	21.285615010423907	21.963720912107966	19.57862742169082
12	24.354226048645977	23.690316848928347	28.75234037247235	23.203116729953326
13	29.41176088601142	19.540305272625144	25.055148431589647	25.992785409773788
14	23.53894512250155	19.738355161959948	24.978329371477102	31.7443703440614
15	25.21544250299054	27.2394006185002	21.986694842498057	25.558462036011203
16	25.72777866879472	25.961340542562223	23.531569812403777	24.779310976239284
17	24.042591737623674	26.08445231448744	25.08972303552493	24.783232912363953
18	24.70758117118164	25.3862071159631	25.88972263469446	24.016489078160802
19	25.658314149680532	25.067288853226554	25.168433120787547	24.105963876305363
20	25.714476751470173	24.682254620851005	24.723216331713257	24.880052295965562
21	26.570838583364655	24.416232220488983	24.424930881349617	24.587998314796746
22	25.89659540128248	24.381435033068254	24.971385425816674	24.75058413983259
23	24.66972562220363	24.495054890488397	25.31005515337077	25.525164333937205
24	24.70914796354888	25.128499409921883	25.29390304542628	24.868449581102958
25	24.799467713928696	24.693270151409422	25.22003862319664	25.287223511465246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	594.0
1	594.0
2	568.0
3	542.0
4	542.0
5	542.0
6	1098.5
7	1655.0
8	1655.0
9	1655.0
10	2024.5
11	2394.0
12	2394.0
13	2394.0
14	3409.5
15	4425.0
16	4425.0
17	4425.0
18	7276.5
19	10128.0
20	10128.0
21	10128.0
22	15945.5
23	21763.0
24	21763.0
25	21763.0
26	32249.0
27	42735.0
28	42735.0
29	42735.0
30	53809.0
31	64883.0
32	64883.0
33	64883.0
34	79584.5
35	94286.0
36	94286.0
37	94286.0
38	109331.0
39	124376.0
40	124376.0
41	124376.0
42	143255.5
43	162135.0
44	162135.0
45	162135.0
46	179130.5
47	196126.0
48	196126.0
49	196126.0
50	202696.5
51	209267.0
52	209267.0
53	209267.0
54	196701.5
55	184136.0
56	184136.0
57	184136.0
58	169370.5
59	154605.0
60	154605.0
61	154605.0
62	135902.5
63	117200.0
64	117200.0
65	117200.0
66	95589.0
67	73978.0
68	73978.0
69	73978.0
70	55931.5
71	37885.0
72	37885.0
73	37885.0
74	28689.0
75	19493.0
76	19493.0
77	19493.0
78	15394.0
79	11295.0
80	11295.0
81	11295.0
82	7825.5
83	4356.0
84	4356.0
85	4356.0
86	3225.0
87	2094.0
88	2094.0
89	2094.0
90	1408.5
91	723.0
92	723.0
93	723.0
94	450.5
95	178.0
96	178.0
97	178.0
98	395.0
99	612.0
100	612.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.431353219220372E-4
2	2.594262528990884E-4
3	9.079918851468093E-4
4	0.009533914794041498
5	0.033336273497532855
6	0.06271629663835461
7	0.1043542102286583
8	0.1090238827808419
9	0.11732552287361273
10	0.13230738897853508
11	0.13840390592166366
12	0.13263167179465893
13	0.12510831046058538
14	0.11421240783882367
15	0.1303616920817919
16	0.11680667036781454
17	0.11979007227615406
18	0.10980216153953916
19	0.09663627920491041
20	0.0908640450779057
21	0.09060461882500662
22	0.10150052144676833
23	0.08113556059418989
24	0.09015062288243321
25	0.08684293815796984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1541864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.32659747447208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88158299534614	38.659718541742784
2	15.050958889486314	15.751309348299552
3	5.107951531434719	8.018451711134933
4	2.1076868945932294	4.411523349423999
5	1.1168906489737707	2.922154370592619
6	0.6350399156314778	1.9937686827282628
7	0.41620026521836584	1.5244840622794964
8	0.2838155667989202	1.1880882336700993
9	0.2046601286293191	0.9638251352874052
>10	1.0349122746016852	9.63631984859092
>50	0.07843651605295043	2.8673275489153958
>100	0.07228462894281948	7.8176169354395695
>500	0.007091499020148897	2.513733496544559
>1k	0.0024882452702276827	1.7316787353504073
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2632	0.17070247440760014	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1646	0.10675390306797486	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1543	0.10007367705582335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.945696896743163E-4
2	0.0	0.0	0.0	0.0	1.945696896743163E-4
3	0.0	0.0	0.0	0.0	1.945696896743163E-4
4	0.0	0.0	0.0	0.0	1.945696896743163E-4
5	0.0	0.0	0.0	0.0	1.945696896743163E-4
6	0.0	0.0	0.0	0.0	3.2428281612386045E-4
7	0.0	0.0	0.0	0.0	3.2428281612386045E-4
8	0.0	0.0	0.0	0.0	3.2428281612386045E-4
9	0.0	0.0	0.0	0.0	3.2428281612386045E-4
10	0.0	0.0	0.0	0.0	3.2428281612386045E-4
11	0.0	0.0	0.0	0.0	3.2428281612386045E-4
12	0.0	0.0	0.0	0.0	0.0010377050115963535
13	0.0	0.0	0.0	0.0	0.001361987827720214
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCGTA	45	6.7524693E-4	14.780607	9
CGTCTTA	65	5.449279E-5	13.1546545	15
GTATTAC	65	5.4742377E-5	13.14825	1
GGTATCA	790	0.0	12.981817	1
CGGTCCA	290	0.0	12.777894	10
TCCAACG	100	1.4454963E-7	12.347954	18
GAATATA	70	1.09522996E-4	12.209089	1
TAACGAA	110	3.8082362E-8	12.092046	13
CGAACGA	110	3.8135113E-8	12.090868	16
CGCGGTC	95	1.0377462E-6	12.001907	10
GTATCAA	1965	0.0	11.88807	1
GCTAGAC	80	2.8692797E-5	11.873802	3
TCGCACG	145	9.640644E-11	11.794212	13
CGCATCG	195	0.0	11.693406	13
ACGGTAT	230	0.0	11.567431	9
CAAGACG	345	0.0	11.564803	4
GTGTAGG	165	7.2759576E-12	11.510252	1
GCCGGTC	315	0.0	11.461394	8
AACCGCG	100	1.9271683E-6	11.4010725	7
GCGTTAT	125	1.8428182E-8	11.39515	1
>>END_MODULE