FastQCFastQC Report
Thu 2 Feb 2017
SRR4062139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541864
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19600.026.9477561
GGTATCA8800.020.5068321
GTATTAC1504.638423E-1019.073021
ACACCGT1303.5914127E-818.6143426
CGCAATA3450.017.85580836
TCAACGC29150.017.7348564
AACGCAG29300.017.7191456
ATCAACG29300.017.6440623
TACACCG750.001291574217.5990145
TCTAGAT4150.017.4946982
GATATAC2900.017.454091
CAACGCA29700.017.4064335
TAGCGGC3550.017.35114130
CGTCTTA1408.381903E-817.28474615
ATCGATT1154.276708E-617.21642929
TATCAAC30350.017.180282
TAGAACA5550.016.6477174
TAGGACG1854.2382453E-1016.6477174
CTAGCGG3750.016.42574729
CGTCGTA4300.016.37117810
Produced by FastQC (version 0.11.5)