FastQCFastQC Report
Thu 2 Feb 2017
SRR4062138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448818
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22630.15619629242596378No Hit
TATCAACGCAGAGTACTTTTTTTTT14820.10229028076680438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCG851.715307E-814.53004613
CGAACGT707.2610364E-613.5715514
CTATACT1001.0068106E-813.3001214
GGTATCA6500.013.1498771
CGTCCAA604.0890244E-412.66765710
TGCTATA604.0973764E-412.6645942
GGTTTAT751.4830115E-512.6628441
GTATCAA17350.012.5898591
CCTATAC1155.326001E-912.3905613
GAACGTC853.9408587E-612.2946545
CGGTCCA2600.012.05863610
GGTCCAA2950.011.91577411
AACGAAC802.8637482E-511.87592914
CGCCTCG802.8647612E-511.87551911
TCGAACG802.867802E-511.8742883
GTCCTAC1552.5465852E-1111.6416471
AACGTCT907.4529653E-611.6120196
GTATATA1501.7644197E-1011.396561
GTATAAA1501.7644197E-1011.396561
CGATAAC855.317801E-511.17734510
Produced by FastQC (version 0.11.5)