##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062138_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1448818
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208311879062794	32.0	32.0	32.0	32.0	32.0
2	30.70707155764216	32.0	32.0	32.0	32.0	32.0
3	30.725250514557384	32.0	32.0	32.0	32.0	32.0
4	30.736993880528818	32.0	32.0	32.0	32.0	32.0
5	30.644111958851976	32.0	32.0	32.0	32.0	32.0
6	34.29221751800433	36.0	36.0	36.0	32.0	36.0
7	34.22709339613395	36.0	36.0	36.0	32.0	36.0
8	34.17581849480059	36.0	36.0	36.0	32.0	36.0
9	34.327288175602455	36.0	36.0	36.0	32.0	36.0
10	33.97838375834646	36.0	36.0	36.0	32.0	36.0
11	34.30448752017161	36.0	36.0	36.0	32.0	36.0
12	34.11788575238574	36.0	36.0	36.0	32.0	36.0
13	34.211892729107454	36.0	36.0	36.0	32.0	36.0
14	34.099303018046434	36.0	36.0	36.0	32.0	36.0
15	34.045515723852134	36.0	36.0	36.0	32.0	36.0
16	34.05380109855068	36.0	36.0	36.0	32.0	36.0
17	33.98550818667355	36.0	36.0	36.0	32.0	36.0
18	33.9972446504668	36.0	36.0	36.0	32.0	36.0
19	34.00225701226793	36.0	36.0	36.0	32.0	36.0
20	33.993592707986785	36.0	36.0	36.0	32.0	36.0
21	33.9659798539223	36.0	36.0	36.0	32.0	36.0
22	33.93886602734091	36.0	36.0	36.0	32.0	36.0
23	33.90297815184516	36.0	36.0	36.0	32.0	36.0
24	33.882457285870274	36.0	36.0	36.0	32.0	36.0
25	33.51788975564909	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	22.0
5	115.0
6	274.0
7	73.0
8	223.0
9	173.0
10	92.0
11	29.0
12	40.0
13	46.0
14	138.0
15	287.0
16	429.0
17	607.0
18	889.0
19	1191.0
20	1905.0
21	2784.0
22	4351.0
23	6551.0
24	9361.0
25	13377.0
26	18064.0
27	23366.0
28	31158.0
29	41703.0
30	54488.0
31	73920.0
32	103177.0
33	144333.0
34	305998.0
35	609654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.1125894185825	17.292401180559967	11.903266418369912	25.691742982487625
2	17.008232929509337	19.08068381354154	37.084350255657036	26.82673300129209
3	19.31603272198187	22.355833607120868	28.083491854015563	30.244641816881696
4	13.23921553468293	15.300607932319124	34.436980950257514	37.023195582740435
5	15.552687958231582	35.70220807295389	32.83470280667814	15.910401162136392
6	35.49695474205951	34.097427535706416	16.257305822074617	14.148311900159463
7	31.399387440245025	29.774715362841125	19.643504869870778	19.18239232704307
8	28.576659633355856	32.7143605283412	18.700246925862825	20.008732912440117
9	26.986362232428686	14.763088960919163	18.47887448513748	39.77167432151467
10	17.009328226697058	26.270934605661623	30.22205851254245	26.497678655098873
11	37.15192874154573	21.36419743700679	21.636779823374354	19.847093998073124
12	24.80713851818527	23.373505466049952	27.92063429537748	23.898721720387307
13	29.456730626372995	19.27684783938183	24.823976632029794	26.442444902215385
14	23.79821527895335	19.40861682391512	24.53352311396857	32.259644783162955
15	25.411879830604057	26.85573978040447	21.710351728789572	26.0220286602019
16	26.139996780031215	25.797408685209355	23.043160710300235	25.019433824459185
17	24.421393630613743	26.014189356807666	24.588689008189252	24.975728004389346
18	25.108630389977744	25.057502077951334	25.317841915404752	24.51602561666617
19	25.74258998918852	24.921884444935703	24.962228892565637	24.373296673310143
20	25.914100461592238	24.263849494127513	24.5157840781764	25.306265966103847
21	26.68506046951172	24.202104326573355	24.290734455705316	24.822100748209607
22	26.259448845826206	24.03967392543452	24.62161435789302	25.079262870846257
23	24.752012256234316	24.335021236357306	25.125694783571507	25.78727172383687
24	24.949726822009808	24.748979511478034	24.931282385701486	25.370011280810672
25	25.13552868621448	24.38024292929851	24.90080571182263	25.583422672664373
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	240.0
1	240.0
2	316.0
3	392.0
4	392.0
5	392.0
6	827.5
7	1263.0
8	1263.0
9	1263.0
10	1548.0
11	1833.0
12	1833.0
13	1833.0
14	2597.5
15	3362.0
16	3362.0
17	3362.0
18	5772.5
19	8183.0
20	8183.0
21	8183.0
22	12954.5
23	17726.0
24	17726.0
25	17726.0
26	26282.5
27	34839.0
28	34839.0
29	34839.0
30	44230.5
31	53622.0
32	53622.0
33	53622.0
34	68256.5
35	82891.0
36	82891.0
37	82891.0
38	97964.5
39	113038.0
40	113038.0
41	113038.0
42	131976.5
43	150915.0
44	150915.0
45	150915.0
46	168125.0
47	185335.0
48	185335.0
49	185335.0
50	192495.0
51	199655.0
52	199655.0
53	199655.0
54	188530.5
55	177406.0
56	177406.0
57	177406.0
58	163986.5
59	150567.0
60	150567.0
61	150567.0
62	132413.5
63	114260.0
64	114260.0
65	114260.0
66	93897.0
67	73534.0
68	73534.0
69	73534.0
70	56290.5
71	39047.0
72	39047.0
73	39047.0
74	29679.5
75	20312.0
76	20312.0
77	20312.0
78	16151.0
79	11990.0
80	11990.0
81	11990.0
82	8367.5
83	4745.0
84	4745.0
85	4745.0
86	3468.0
87	2191.0
88	2191.0
89	2191.0
90	1436.0
91	681.0
92	681.0
93	681.0
94	430.5
95	180.0
96	180.0
97	180.0
98	395.5
99	611.0
100	611.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.663049465150213E-4
2	1.3804356378786017E-4
3	4.1413069136358056E-4
4	0.009455984119468422
5	0.031128823634162472
6	0.05721905719006804
7	0.10001256196430469
8	0.09801093028938074
9	0.10953756786566705
10	0.1250674687918013
11	0.13224573410877002
12	0.1238940984996045
13	0.11795822525672653
14	0.10581039164339481
15	0.12382507671771056
16	0.11036582924839419
17	0.11574952823612075
18	0.1020832154211226
19	0.0885549461699123
20	0.0842065739105947
21	0.08482776994764007
22	0.0979419085074868
23	0.07350819771703554
24	0.08468972638385222
25	0.07965113630559532
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1448818.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.92431338613804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10025036547968	45.818276910920254
2	12.598778296398356	14.595511646461052
3	3.729819766109326	6.481417470177861
4	1.5060715011973334	3.489526304691428
5	0.7824840950609655	2.266242697099
6	0.501101917540214	1.7415590705996435
7	0.3292103777732358	1.3348499564474061
8	0.24115684218709144	1.1175075601645215
9	0.16892026052210085	0.8806131096975226
>10	0.9120114860424161	9.335578159560233
>50	0.06484803454722987	2.6104309674002026
>100	0.05816614651931147	6.925188832570023
>500	0.005266001123319459	2.0249799264258637
>1k	0.0019149094993888942	1.3783173877849715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2263	0.15619629242596378	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1482	0.10229028076680438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.902178189393008E-5
2	0.0	0.0	0.0	0.0	6.902178189393008E-5
3	0.0	0.0	0.0	0.0	6.902178189393008E-5
4	0.0	0.0	0.0	0.0	6.902178189393008E-5
5	0.0	0.0	0.0	0.0	6.902178189393008E-5
6	6.902178189393008E-5	0.0	0.0	0.0	6.902178189393008E-5
7	6.902178189393008E-5	0.0	0.0	0.0	6.902178189393008E-5
8	6.902178189393008E-5	0.0	0.0	0.0	6.902178189393008E-5
9	6.902178189393008E-5	0.0	0.0	0.0	6.902178189393008E-5
10	6.902178189393008E-5	0.0	0.0	0.0	6.902178189393008E-5
11	6.902178189393008E-5	0.0	0.0	0.0	6.902178189393008E-5
12	6.902178189393008E-5	0.0	0.0	0.0	6.902178189393008E-5
13	6.902178189393008E-5	0.0	0.0	0.0	1.3804356378786017E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCGCG	85	1.715307E-8	14.530046	13
CGAACGT	70	7.2610364E-6	13.571551	4
CTATACT	100	1.0068106E-8	13.300121	4
GGTATCA	650	0.0	13.149877	1
CGTCCAA	60	4.0890244E-4	12.667657	10
TGCTATA	60	4.0973764E-4	12.664594	2
GGTTTAT	75	1.4830115E-5	12.662844	1
GTATCAA	1735	0.0	12.589859	1
CCTATAC	115	5.326001E-9	12.390561	3
GAACGTC	85	3.9408587E-6	12.294654	5
CGGTCCA	260	0.0	12.058636	10
GGTCCAA	295	0.0	11.915774	11
AACGAAC	80	2.8637482E-5	11.875929	14
CGCCTCG	80	2.8647612E-5	11.875519	11
TCGAACG	80	2.867802E-5	11.874288	3
GTCCTAC	155	2.5465852E-11	11.641647	1
AACGTCT	90	7.4529653E-6	11.612019	6
GTATATA	150	1.7644197E-10	11.39656	1
GTATAAA	150	1.7644197E-10	11.39656	1
CGATAAC	85	5.317801E-5	11.177345	10
>>END_MODULE