FastQCFastQC Report
Thu 2 Feb 2017
SRR4062138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448818
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16700.027.6700531
AGCGCGA407.0304883E-427.50155438
GGTATCA7800.025.9537681
TTTATAC1951.8189894E-1218.0504323
TATCAAC25100.017.7926062
TCAACGC25150.017.756624
ATCAACG25350.017.6165283
GTATAGA1751.9099389E-1017.6034261
TATACTG2250.017.599175
CAACGCA25500.017.426635
AACGCAG25800.017.3092636
GTATAGC2350.016.8543431
ATACCGT5000.016.7192136
ACCGTCG5400.016.7029198
CGTCGTA5050.016.55367510
CGGATCG800.00198785616.50093326
GGACCGT1206.4111664E-616.4992246
TACCGTC5600.016.4992247
AACCGCG2700.016.2977841
TAACGCC3400.016.1757094
Produced by FastQC (version 0.11.5)