##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1448818
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275303730351226	32.0	32.0	32.0	32.0	32.0
2	31.425423345099247	32.0	32.0	32.0	32.0	32.0
3	31.555450719137944	32.0	32.0	32.0	32.0	32.0
4	31.63447237679267	32.0	32.0	32.0	32.0	32.0
5	31.610668834870907	32.0	32.0	32.0	32.0	32.0
6	35.2478496263851	36.0	36.0	36.0	36.0	36.0
7	35.249125148914494	36.0	36.0	36.0	36.0	36.0
8	35.200381276323185	36.0	36.0	36.0	36.0	36.0
9	35.28663572650257	36.0	36.0	36.0	36.0	36.0
10	35.15657246113729	36.0	36.0	36.0	36.0	36.0
11	35.2816040386025	36.0	36.0	36.0	36.0	36.0
12	35.20446046363311	36.0	36.0	36.0	36.0	36.0
13	35.23070875706956	36.0	36.0	36.0	36.0	36.0
14	35.20400146878352	36.0	36.0	36.0	36.0	36.0
15	35.18463050569499	36.0	36.0	36.0	36.0	36.0
16	35.191001906381615	36.0	36.0	36.0	36.0	36.0
17	35.16829443035633	36.0	36.0	36.0	36.0	36.0
18	35.16556806997152	36.0	36.0	36.0	36.0	36.0
19	35.158282820892616	36.0	36.0	36.0	36.0	36.0
20	35.15370943762433	36.0	36.0	36.0	36.0	36.0
21	35.150227978945594	36.0	36.0	36.0	36.0	36.0
22	35.13562296989684	36.0	36.0	36.0	36.0	36.0
23	35.10154829661145	36.0	36.0	36.0	36.0	36.0
24	35.085112139689045	36.0	36.0	36.0	36.0	36.0
25	35.07034078814592	36.0	36.0	36.0	36.0	36.0
26	35.027996615171816	36.0	36.0	36.0	36.0	36.0
27	35.01068457183718	36.0	36.0	36.0	36.0	36.0
28	34.99332490347304	36.0	36.0	36.0	36.0	36.0
29	34.97911884032363	36.0	36.0	36.0	36.0	36.0
30	34.950599040045056	36.0	36.0	36.0	36.0	36.0
31	34.961407851089646	36.0	36.0	36.0	36.0	36.0
32	34.93889777736058	36.0	36.0	36.0	32.0	36.0
33	34.91523987139862	36.0	36.0	36.0	32.0	36.0
34	34.905567158884	36.0	36.0	36.0	32.0	36.0
35	34.895561761380655	36.0	36.0	36.0	32.0	36.0
36	34.86861841860054	36.0	36.0	36.0	32.0	36.0
37	34.85946613032141	36.0	36.0	36.0	32.0	36.0
38	34.839738324620484	36.0	36.0	36.0	32.0	36.0
39	34.825866326895444	36.0	36.0	36.0	32.0	36.0
40	34.8109921328973	36.0	36.0	36.0	32.0	36.0
41	34.79138994683942	36.0	36.0	36.0	32.0	36.0
42	34.759573666257594	36.0	36.0	36.0	32.0	36.0
43	34.75629927292455	36.0	36.0	36.0	32.0	36.0
44	34.70863352056642	36.0	36.0	36.0	32.0	36.0
45	34.697982769402365	36.0	36.0	36.0	32.0	36.0
46	34.68479684818935	36.0	36.0	36.0	32.0	36.0
47	34.65748009756919	36.0	36.0	36.0	32.0	36.0
48	34.63882765123017	36.0	36.0	36.0	32.0	36.0
49	34.625130968831144	36.0	36.0	36.0	32.0	36.0
50	34.21016028238191	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	15.0
21	35.0
22	95.0
23	294.0
24	676.0
25	1684.0
26	3513.0
27	6371.0
28	11340.0
29	18245.0
30	27302.0
31	40579.0
32	62451.0
33	105174.0
34	240851.0
35	930191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.676025366183715	17.812667212101754	12.359144840632842	26.15216258108169
2	16.308838978921024	19.65739931609631	36.81449146915048	27.219270235832195
3	18.597129378146967	22.885530588791532	28.40645631084714	30.11088372221436
4	12.965741728774766	15.742142905458104	34.76889436768455	36.52322099808257
5	15.169469181084166	36.08935007709733	32.731854518648994	16.009326223169506
6	35.572860083185056	34.32025278537401	16.1424692404429	13.96441789099804
7	31.320773209609488	29.834527180087495	19.730221463289386	19.11447814701363
8	28.509792120197293	32.80315401934543	18.7123572456996	19.974696614757686
9	27.1395026842571	14.493262783869335	18.474991337766372	39.892243194107195
10	16.938578164115963	26.332311120037932	30.328813093717148	26.40029762212895
11	37.35610684019663	21.204595746325623	21.63425633861534	19.805041074862405
12	24.864492893167323	23.29417724943868	28.002018198295573	23.83931165909842
13	29.455252488580346	19.25894073651763	25.01570245538087	26.270104319521153
14	23.811686492023153	19.26715432856301	24.668798979581975	32.25236019983186
15	25.431351625946114	26.879014479389408	21.742068361933658	25.94756553273082
16	26.17368413060614	25.746023989276743	23.0986862357763	24.98160564434082
17	24.517089102979117	25.967167718788698	24.59480762939168	24.920935548840504
18	25.1744009077751	24.972995209194814	25.389403906773595	24.46319997625649
19	25.87875081618257	24.803322432493246	24.973599168425572	24.34432758289861
20	26.13035636759195	24.14866514864237	24.523799827859083	25.197178655906594
21	26.85052701617941	24.04375431817821	24.280913324457128	24.824805341185257
22	26.35203317462925	23.869664788814056	24.740650654533557	25.037651382023142
23	24.817558049084184	24.08834242005719	25.218505856847344	25.875593674011277
24	25.091092565507616	24.56472628055889	25.008610568325118	25.335570585608373
25	25.24342340937029	24.234459519620543	24.95946681869917	25.562650252309993
26	25.035770612535075	25.018239043632214	25.13267739497451	24.813312948858204
27	25.49776955342063	24.5557570231631	24.702911970005793	25.24356145341048
28	25.18749974116546	24.396708452914893	25.137665464293946	25.2781263416257
29	25.06191279475874	24.76076951322877	25.01028428809259	25.167033403919902
30	24.989577581446714	24.817020982882383	25.15302319160685	25.040378244064055
31	25.59065716001408	24.485129174978084	24.452826802686342	25.471386862321495
32	25.442588281254853	24.454188860213954	24.48373040715568	25.619492451375507
33	25.005193899842286	24.345206254766826	24.93575784347898	25.713842001911907
34	25.54500125620857	24.36892775006143	25.042172716254235	25.04389827747577
35	25.916319083277138	24.29961630659029	25.090608656879805	24.693455953252766
36	24.894189170161486	24.76960433789891	24.84766829650776	25.488538195431843
37	25.83847381924126	24.53373256220868	24.71961142827167	24.908182190278392
38	25.078374233340554	24.38049499661103	25.018739413784203	25.52239135626421
39	25.704906581888988	24.285119708671054	24.584261969659217	25.425711739780745
40	25.823016863425032	24.47060220207397	24.809637662753588	24.89674327174741
41	25.033631397126577	24.986557794581014	25.050334933513714	24.929475874778696
42	25.974760153816312	24.739204592125162	24.66866058184313	24.61737467221539
43	25.54365795088321	24.084470002091457	24.906626466521853	25.46524558050348
44	25.151803593035915	24.625417688526706	24.688989865035097	25.533788853402285
45	25.31664354392286	24.783232904699783	24.675694890218736	25.224428661158623
46	25.201307028938793	24.542960914754598	24.781506120313477	25.474225935993132
47	25.228585568342375	24.583983583666768	25.056029088881083	25.13140175910977
48	25.859925632903746	25.029559781854676	24.12264565742128	24.98786892782029
49	25.23951384900826	25.088834099041392	24.278835534744005	25.392816517206345
50	25.456207024601152	25.127864969070828	24.290271770865974	25.125656235462046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	131.0
1	80.5
2	30.0
3	90.0
4	150.0
5	175.5
6	201.0
7	161.5
8	122.0
9	165.0
10	208.0
11	356.5
12	505.0
13	934.5
14	1364.0
15	1825.0
16	2286.0
17	2202.5
18	2119.0
19	2196.5
20	2274.0
21	2701.5
22	3129.0
23	3888.5
24	4648.0
25	5944.5
26	7241.0
27	9309.0
28	11377.0
29	13260.0
30	15143.0
31	17772.0
32	20401.0
33	23574.5
34	26748.0
35	31382.0
36	36016.0
37	42335.5
38	48655.0
39	54183.5
40	59712.0
41	65651.5
42	71591.0
43	75484.5
44	79378.0
45	86651.5
46	93925.0
47	100474.5
48	107024.0
49	111029.5
50	115035.0
51	112664.5
52	110294.0
53	109921.5
54	109549.0
55	109652.5
56	109756.0
57	106063.5
58	102371.0
59	94300.0
60	86229.0
61	75680.5
62	65132.0
63	56419.0
64	47706.0
65	40661.5
66	33617.0
67	29146.0
68	24675.0
69	21791.5
70	18908.0
71	14931.0
72	10954.0
73	9534.5
74	8115.0
75	6119.5
76	4124.0
77	3610.5
78	3097.0
79	2652.0
80	2207.0
81	1701.5
82	1196.0
83	1004.0
84	812.0
85	604.0
86	396.0
87	274.5
88	153.0
89	101.0
90	49.0
91	32.0
92	15.0
93	13.5
94	12.0
95	14.0
96	16.0
97	16.0
98	16.0
99	11.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029886431560071723
2	0.005245655423938687
3	2.0706534568179028E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	6.902178189393008E-5
11	0.0
12	6.902178189393008E-5
13	0.0
14	0.0
15	0.0
16	2.7608712757572033E-4
17	0.0
18	6.902178189393008E-5
19	0.0
20	3.4510890946965047E-4
21	6.902178189393008E-5
22	0.0
23	6.902178189393008E-5
24	0.0011733702921968116
25	3.4510890946965047E-4
26	2.0706534568179028E-4
27	3.4510890946965047E-4
28	0.0011043485103028813
29	4.1413069136358056E-4
30	0.0012423920740907417
31	5.521742551514407E-4
32	7.592396008332309E-4
33	2.0706534568179028E-4
34	9.663049465150213E-4
35	3.4510890946965047E-4
36	4.1413069136358056E-4
37	0.001725544547348252
38	0.0
39	6.211960370453709E-4
40	1.3804356378786017E-4
41	0.001587500983560392
42	0.005314677205832617
43	0.004624459386893315
44	0.0049005465144690355
45	0.0019326098930300426
46	0.0024847841481814834
47	0.0011733702921968116
48	0.0040722851317418745
49	0.003451089094696504
50	0.0016565227654543222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1448818.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.01369498882018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.64891705368092	46.41363202206943
2	13.16223224650573	15.53503918334605
3	3.6506940382625244	6.463228334145866
4	1.53336315600797	3.619577023829961
5	0.7977143169526693	2.353803469442992
6	0.47703390609530544	1.6890920060180306
7	0.3266165349628234	1.3492394000820966
8	0.23490199198306336	1.1089947605723827
9	0.1608954685482336	0.8545532495389893
>10	0.892150273710462	9.173730601018846
>50	0.059554606194941184	2.4584732667617857
>100	0.049568529179548126	6.066985731868304
>500	0.005298231596559764	2.144695567175543
>1k	0.0010596463193119528	0.7689553841297092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3804356378786017E-4
2	0.0	0.0	0.0	0.0	1.3804356378786017E-4
3	0.0	0.0	0.0	0.0	1.3804356378786017E-4
4	0.0	0.0	0.0	0.0	1.3804356378786017E-4
5	0.0	0.0	0.0	0.0	1.3804356378786017E-4
6	0.0	0.0	0.0	0.0	2.0706534568179025E-4
7	0.0	0.0	0.0	0.0	2.0706534568179025E-4
8	0.0	0.0	0.0	0.0	2.0706534568179025E-4
9	0.0	0.0	0.0	0.0	2.0706534568179025E-4
10	0.0	0.0	0.0	0.0	2.7608712757572033E-4
11	0.0	0.0	0.0	0.0	2.7608712757572033E-4
12	0.0	0.0	0.0	1.3804356378786017E-4	2.7608712757572033E-4
13	0.0	0.0	0.0	1.3804356378786017E-4	2.7608712757572033E-4
14	0.0	0.0	0.0	2.0706534568179025E-4	2.7608712757572033E-4
15	0.0	0.0	0.0	2.0706534568179025E-4	3.451089094696504E-4
16	6.902178189393008E-5	0.0	0.0	5.521742551514407E-4	3.451089094696504E-4
17	6.902178189393008E-5	0.0	0.0	6.902178189393008E-4	3.451089094696504E-4
18	6.902178189393008E-5	0.0	0.0	9.663049465150212E-4	3.451089094696504E-4
19	6.902178189393008E-5	0.0	0.0	0.0010353267284089513	3.451089094696504E-4
20	6.902178189393008E-5	6.902178189393008E-5	0.0	0.0013804356378786017	4.141306913635805E-4
21	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.001587500983560392	4.141306913635805E-4
22	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.001656522765454322	4.141306913635805E-4
23	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.0018635881111361123	4.141306913635805E-4
24	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.0021396752387118326	4.141306913635805E-4
25	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.0027608712757572033	4.141306913635805E-4
26	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.003244023749014714	4.831524732575106E-4
27	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.004279350477423665	4.831524732575106E-4
28	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.004831524732575106	4.831524732575106E-4
29	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.0058668514609840575	4.831524732575106E-4
30	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.00807554848158982	4.831524732575106E-4
31	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.013183160341740647	4.831524732575106E-4
32	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.019119033584618635	5.521742551514407E-4
33	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.02436468900855732	5.521742551514407E-4
34	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.03023154046954138	6.902178189393008E-4
35	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.037064696877040455	6.902178189393008E-4
36	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.046865789905978526	6.902178189393008E-4
37	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.06218862548643101	6.902178189393008E-4
38	2.0706534568179025E-4	6.902178189393008E-5	0.0	0.08365439965544326	8.28261382727161E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1670	0.0	27.670053	1
AGCGCGA	40	7.0304883E-4	27.501554	38
GGTATCA	780	0.0	25.953768	1
TTTATAC	195	1.8189894E-12	18.050432	3
TATCAAC	2510	0.0	17.792606	2
TCAACGC	2515	0.0	17.75662	4
ATCAACG	2535	0.0	17.616528	3
GTATAGA	175	1.9099389E-10	17.603426	1
TATACTG	225	0.0	17.59917	5
CAACGCA	2550	0.0	17.42663	5
AACGCAG	2580	0.0	17.309263	6
GTATAGC	235	0.0	16.854343	1
ATACCGT	500	0.0	16.719213	6
ACCGTCG	540	0.0	16.702919	8
CGTCGTA	505	0.0	16.553675	10
CGGATCG	80	0.001987856	16.500933	26
GGACCGT	120	6.4111664E-6	16.499224	6
TACCGTC	560	0.0	16.499224	7
AACCGCG	270	0.0	16.29778	41
TAACGCC	340	0.0	16.175709	4
>>END_MODULE