FastQCFastQC Report
Thu 2 Feb 2017
SRR4062137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261199
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18950.15025384574519962No Hit
TATCAACGCAGAGTACTTTTTTTTT13190.10458301980892784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG250.00603007319.00075512
CGTCCGG508.700049E-515.202414510
CGTCTTA801.2889905E-714.24886715
GGTATCA4650.013.8875091
GTCCTAC3450.012.9374051
AGAACCG604.0855876E-412.6686795
CTTATAC905.407437E-712.6656613
GTCTAAG604.1034195E-412.6621411
GGATATA701.0942276E-412.2099211
AATACCG550.00306424112.0928315
TCCTACA4250.011.8447072
TGGACCG907.444127E-611.6129565
GCATATA1104.9877053E-711.2232611
TTATACT855.3121606E-511.17824654
GGACGTG3850.011.1061016
GAACCGC600.005865930611.0855356
CGGCCAA600.00586766511.08509410
TTTATAC1201.27553E-711.0824533
AGGACGT3650.010.9332445
ACCGTGG700.001489086410.8588698
Produced by FastQC (version 0.11.5)