##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062137_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1261199
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214902644229817	32.0	32.0	32.0	32.0	32.0
2	30.748198341419553	32.0	32.0	32.0	32.0	32.0
3	30.752802690138513	32.0	32.0	32.0	32.0	32.0
4	30.754318707832784	32.0	32.0	32.0	32.0	32.0
5	30.649481961213098	32.0	32.0	32.0	32.0	32.0
6	34.31715772055005	36.0	36.0	36.0	32.0	36.0
7	34.260344323140124	36.0	36.0	36.0	32.0	36.0
8	34.20124024836683	36.0	36.0	36.0	32.0	36.0
9	34.353299518949825	36.0	36.0	36.0	32.0	36.0
10	34.007309710838655	36.0	36.0	36.0	32.0	36.0
11	34.33843509232088	36.0	36.0	36.0	32.0	36.0
12	34.16019200776404	36.0	36.0	36.0	32.0	36.0
13	34.233238370788435	36.0	36.0	36.0	32.0	36.0
14	34.14080331494078	36.0	36.0	36.0	32.0	36.0
15	34.07305270619466	36.0	36.0	36.0	32.0	36.0
16	34.08454970230709	36.0	36.0	36.0	32.0	36.0
17	34.0376942893231	36.0	36.0	36.0	32.0	36.0
18	34.0309768720083	36.0	36.0	36.0	32.0	36.0
19	34.03134160429877	36.0	36.0	36.0	32.0	36.0
20	34.002453221101504	36.0	36.0	36.0	32.0	36.0
21	33.99979384696626	36.0	36.0	36.0	32.0	36.0
22	33.97315728921447	36.0	36.0	36.0	32.0	36.0
23	33.92974542479022	36.0	36.0	36.0	32.0	36.0
24	33.90012599121947	36.0	36.0	36.0	32.0	36.0
25	33.53394428634974	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	15.0
5	103.0
6	242.0
7	40.0
8	231.0
9	155.0
10	74.0
11	30.0
12	58.0
13	57.0
14	138.0
15	233.0
16	396.0
17	552.0
18	768.0
19	1082.0
20	1669.0
21	2552.0
22	3857.0
23	5718.0
24	8151.0
25	11216.0
26	15644.0
27	20132.0
28	26589.0
29	35393.0
30	46074.0
31	62405.0
32	87996.0
33	123257.0
34	261780.0
35	544589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52468866606512	16.696823466165974	11.184710264782515	26.593777602986396
2	17.165980282239584	18.29768204516658	37.01290360435078	27.523434068243052
3	19.923357976764763	21.217534578508516	27.59223842916361	31.266869015563103
4	13.62918356374686	14.913335225912046	33.51957788093565	37.93790332940544
5	15.506961877228257	35.32271305009934	33.31580471051995	15.854520362152453
6	36.50200008568395	33.26132178609794	15.792029170626906	14.444648957591202
7	31.762334931662878	29.312691837248195	19.005569679733494	19.919403551355433
8	28.626082649665996	33.58658657387417	17.957300424170075	19.830030352289757
9	26.91677204814763	14.687439718220638	18.021225537008945	40.37456269662279
10	17.026444012007662	26.546351850572712	30.799846609327773	25.62735752809185
11	37.23339891668373	20.755621827028282	21.337773310275942	20.67320594601204
12	24.631040913591047	22.980460590509203	28.08169856544801	24.30679993045174
13	30.607408748172105	19.21492228769035	23.91544926114162	26.26221970299592
14	23.37407086361659	18.849760115322457	24.516341052190395	33.25982796887056
15	25.56868704190643	26.657473038375063	21.14780623197795	26.62603368774056
16	25.929374669568904	25.367979182642465	23.067067391273284	25.635578756515347
17	24.650622227444057	25.65731605470648	24.388886595515643	25.30317512233382
18	25.25634085435839	25.025598366784514	25.006469044629263	24.711591734227834
19	26.20587467331842	24.54947107940436	24.5557408261304	24.688913421146825
20	26.034637510822733	24.416405638059203	24.703054332307737	24.845902518810327
21	25.97208730929661	24.108304922258412	24.345116786977254	25.574490981467722
22	26.091670390427378	24.178464307852757	24.151637356767495	25.578227944952364
23	25.370555966865606	24.422929358094464	24.589569441700174	25.61694523333976
24	25.35453929334982	24.358020008158174	24.435316319679767	25.852124378812242
25	25.525812922967532	24.213343871811478	24.524498787440166	25.736344417780828
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	45.0
2	187.0
3	329.0
4	329.0
5	329.0
6	735.5
7	1142.0
8	1142.0
9	1142.0
10	1337.0
11	1532.0
12	1532.0
13	1532.0
14	2246.5
15	2961.0
16	2961.0
17	2961.0
18	4982.0
19	7003.0
20	7003.0
21	7003.0
22	10771.5
23	14540.0
24	14540.0
25	14540.0
26	21356.5
27	28173.0
28	28173.0
29	28173.0
30	36685.5
31	45198.0
32	45198.0
33	45198.0
34	55984.0
35	66770.0
36	66770.0
37	66770.0
38	78744.5
39	90719.0
40	90719.0
41	90719.0
42	106648.5
43	122578.0
44	122578.0
45	122578.0
46	139021.0
47	155464.0
48	155464.0
49	155464.0
50	163129.0
51	170794.0
52	170794.0
53	170794.0
54	167905.5
55	165017.0
56	165017.0
57	165017.0
58	153189.5
59	141362.0
60	141362.0
61	141362.0
62	123632.0
63	105902.0
64	105902.0
65	105902.0
66	87939.0
67	69976.0
68	69976.0
69	69976.0
70	53539.0
71	37102.0
72	37102.0
73	37102.0
74	27659.5
75	18217.0
76	18217.0
77	18217.0
78	13734.0
79	9251.0
80	9251.0
81	9251.0
82	6716.5
83	4182.0
84	4182.0
85	4182.0
86	2879.0
87	1576.0
88	1576.0
89	1576.0
90	1146.0
91	716.0
92	716.0
93	716.0
94	456.0
95	196.0
96	196.0
97	196.0
98	325.0
99	454.0
100	454.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010307651687005777
2	7.92896283615829E-5
3	8.721859119774119E-4
4	0.010545520572090527
5	0.0320330098580795
6	0.05970509015627192
7	0.10640668126124427
8	0.10521733683582053
9	0.11814154625875853
10	0.13241367936384346
11	0.13740892595062318
12	0.13019356976971913
13	0.12313679284553826
14	0.11314629967197881
15	0.12932138385774172
16	0.1174279396035043
17	0.12139242102158343
18	0.10727886717322169
19	0.09356176146666782
20	0.08880438376497286
21	0.08935941116350393
22	0.10085640727593345
23	0.07913104910485974
24	0.08824935636644177
25	0.08365055792146996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1261199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.82082676341802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.0274070846455	28.249404942883217
2	16.42173644901947	14.720716090720181
3	7.632270949379941	10.26254082100879
4	4.179797701204457	7.493679546872714
5	2.4528273669452	5.496887524721078
6	1.5678266636246736	4.216277237123349
7	1.0351167965380634	3.247635343227573
8	0.7507120051755953	2.691802618655482
9	0.5438520601583017	2.1938309075954914
>10	2.281262061470449	16.730138656321493
>50	0.08269114367802664	2.5105240733230096
>100	0.023256984721228318	1.6780977035253803
>500	8.876667422657772E-4	0.2527204006345694
>1k	3.550666969063109E-4	0.25574413338775376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1895	0.15025384574519962	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1319	0.10458301980892784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.585792567231658E-4
2	0.0	0.0	0.0	0.0	1.585792567231658E-4
3	0.0	0.0	0.0	0.0	2.3786888508474873E-4
4	0.0	0.0	0.0	0.0	2.3786888508474873E-4
5	0.0	0.0	0.0	0.0	2.3786888508474873E-4
6	0.0014272133105084923	0.0	0.0	0.0	3.171585134463316E-4
7	0.0014272133105084923	0.0	0.0	0.0	3.171585134463316E-4
8	0.0014272133105084923	0.0	0.0	0.0	3.171585134463316E-4
9	0.0014272133105084923	0.0	0.0	0.0	3.171585134463316E-4
10	0.0014272133105084923	0.0	0.0	0.0	3.964481418079145E-4
11	0.0015065029388700753	0.0	0.0	0.0	4.7573777016949746E-4
12	0.0015065029388700753	0.0	0.0	0.0	8.721859119774119E-4
13	0.0015065029388700753	0.0	0.0	0.0	0.0010307651687005777
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAACG	25	0.006030073	19.000755	12
CGTCCGG	50	8.700049E-5	15.2024145	10
CGTCTTA	80	1.2889905E-7	14.248867	15
GGTATCA	465	0.0	13.887509	1
GTCCTAC	345	0.0	12.937405	1
AGAACCG	60	4.0855876E-4	12.668679	5
CTTATAC	90	5.407437E-7	12.665661	3
GTCTAAG	60	4.1034195E-4	12.662141	1
GGATATA	70	1.0942276E-4	12.209921	1
AATACCG	55	0.003064241	12.092831	5
TCCTACA	425	0.0	11.844707	2
TGGACCG	90	7.444127E-6	11.612956	5
GCATATA	110	4.9877053E-7	11.223261	1
TTATACT	85	5.3121606E-5	11.1782465	4
GGACGTG	385	0.0	11.106101	6
GAACCGC	60	0.0058659306	11.085535	6
CGGCCAA	60	0.005867665	11.085094	10
TTTATAC	120	1.27553E-7	11.082453	3
AGGACGT	365	0.0	10.933244	5
ACCGTGG	70	0.0014890864	10.858869	8
>>END_MODULE