FastQCFastQC Report
Thu 2 Feb 2017
SRR4062137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261199
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6250.024.2978971
TATACTG1850.023.7830985
GTATCAA10850.023.5303211
TAGGACG3650.023.506174
GTCTACG752.0570878E-623.4762291
TCCGCTA654.9319665E-420.30791338
TGTAGGA5750.019.5132542
AGGACGT4700.018.2547935
GTCCTAC5550.018.2416631
TTAGGAC2900.017.4477733
TCCTATA1154.2727406E-617.2175772
GTACCGT902.2159542E-417.1106196
CTACAGT5400.017.1106174
GTAGGAC5050.016.5539783
ATCAACG15800.016.290673
TATCAAC15700.016.2549512
TCAACGC15700.016.2543094
ACAATAC1501.8383616E-716.1328683
CAACGCA16050.016.036925
CTGTAGG6200.015.9742481
Produced by FastQC (version 0.11.5)