##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1261199
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201354425431674	32.0	32.0	32.0	32.0	32.0
2	31.406824775471595	32.0	32.0	32.0	32.0	32.0
3	31.537349775887865	32.0	32.0	32.0	32.0	32.0
4	31.596126384496024	32.0	32.0	32.0	32.0	32.0
5	31.595668090444093	32.0	32.0	32.0	32.0	32.0
6	35.1792564060073	36.0	36.0	36.0	36.0	36.0
7	35.195276875417754	36.0	36.0	36.0	36.0	36.0
8	35.13671038432476	36.0	36.0	36.0	36.0	36.0
9	35.266654191765134	36.0	36.0	36.0	36.0	36.0
10	35.123804411516346	36.0	36.0	36.0	36.0	36.0
11	35.245258678448046	36.0	36.0	36.0	36.0	36.0
12	35.182283684018145	36.0	36.0	36.0	36.0	36.0
13	35.203660167824424	36.0	36.0	36.0	36.0	36.0
14	35.15275939800143	36.0	36.0	36.0	36.0	36.0
15	35.12346822349209	36.0	36.0	36.0	36.0	36.0
16	35.16057973404673	36.0	36.0	36.0	36.0	36.0
17	35.152461268998785	36.0	36.0	36.0	36.0	36.0
18	35.12667945344074	36.0	36.0	36.0	36.0	36.0
19	35.135338673754106	36.0	36.0	36.0	36.0	36.0
20	35.126964896102834	36.0	36.0	36.0	36.0	36.0
21	35.12820498589041	36.0	36.0	36.0	36.0	36.0
22	35.09654463728563	36.0	36.0	36.0	36.0	36.0
23	35.04776565791759	36.0	36.0	36.0	36.0	36.0
24	35.052140859610574	36.0	36.0	36.0	36.0	36.0
25	35.04070808809712	36.0	36.0	36.0	36.0	36.0
26	34.99788455271531	36.0	36.0	36.0	36.0	36.0
27	34.97737787613216	36.0	36.0	36.0	36.0	36.0
28	34.9623881718904	36.0	36.0	36.0	36.0	36.0
29	34.91907304081275	36.0	36.0	36.0	32.0	36.0
30	34.92073970880091	36.0	36.0	36.0	32.0	36.0
31	34.899551141413845	36.0	36.0	36.0	32.0	36.0
32	34.846087730802196	36.0	36.0	36.0	32.0	36.0
33	34.821346195168246	36.0	36.0	36.0	32.0	36.0
34	34.80494196395652	36.0	36.0	36.0	32.0	36.0
35	34.78733808066768	36.0	36.0	36.0	32.0	36.0
36	34.75933456972294	36.0	36.0	36.0	32.0	36.0
37	34.763387062628496	36.0	36.0	36.0	32.0	36.0
38	34.762414178888505	36.0	36.0	36.0	32.0	36.0
39	34.739399571360266	36.0	36.0	36.0	32.0	36.0
40	34.75454230458477	36.0	36.0	36.0	32.0	36.0
41	34.74607179358689	36.0	36.0	36.0	32.0	36.0
42	34.7294431727269	36.0	36.0	36.0	32.0	36.0
43	34.71663393326509	36.0	36.0	36.0	32.0	36.0
44	34.68249181929259	36.0	36.0	36.0	32.0	36.0
45	34.67302860214764	36.0	36.0	36.0	32.0	36.0
46	34.638816713302184	36.0	36.0	36.0	32.0	36.0
47	34.62321092864806	36.0	36.0	36.0	32.0	36.0
48	34.58766221666843	36.0	36.0	36.0	32.0	36.0
49	34.57606293693541	36.0	36.0	36.0	32.0	36.0
50	34.1303251905528	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	14.0
21	31.0
22	75.0
23	277.0
24	669.0
25	1561.0
26	3477.0
27	6560.0
28	10891.0
29	17124.0
30	25633.0
31	37456.0
32	56095.0
33	94382.0
34	212205.0
35	794742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.001297556928726	17.239106589225678	11.628997066220611	27.130598787624987
2	16.542666183510143	18.77071024801947	36.75166574026425	27.934957828206137
3	19.381444279874025	21.680135363575527	27.780456011595344	31.157964344955108
4	13.402981453333618	15.33336980662022	33.57437418579334	37.689274554252826
5	15.107925077644369	35.81417365538666	33.09493585072617	15.982965416242797
6	36.663214916634686	33.4085265097574	15.674248887563868	14.254009686044041
7	31.971084658329097	29.200863622632113	19.026973538672326	19.80107818036646
8	28.619512067484987	33.64211357604946	18.009211869023048	19.729162487442505
9	27.052986879945195	14.46631340494244	17.841355725781575	40.63934398933079
10	16.985437655308683	26.48584916880617	30.984746249201155	25.543966926683993
11	37.579795099742384	20.603568509014043	21.237806246278343	20.578830144965227
12	24.714180712163582	22.82700826752955	28.21489709395583	24.243913926351034
13	30.77539706263643	19.125054808955603	24.00771012346188	26.091838004946087
14	23.40415747237351	18.65930753195967	24.46552843762166	33.47100655804516
15	25.592392635896477	26.7217148126505	21.10634404245484	26.579548508998187
16	26.1416805887143	25.278288979685044	23.08226602039821	25.497764411202446
17	24.73043508597771	25.543312355940657	24.472268056032394	25.253984502049242
18	25.38181950811847	24.91361388140482	24.93977947951075	24.764787130965953
19	26.386954001707895	24.42398067236019	24.490980408325726	24.698084917606185
20	26.187523588720545	24.273150247859967	24.61710947386449	24.922216689554993
21	26.180087361312527	23.843659882381765	24.315433171133183	25.660819585172522
22	26.282212402642248	24.183257360654427	24.07835718233205	25.456173054371277
23	25.540775087833083	24.341440169235785	24.541884349733863	25.575900393197266
24	25.575944604654815	24.226345139388307	24.353130260412993	25.84457999554389
25	25.70720626072891	24.114034705180405	24.59143907167409	25.5873199624166
26	25.460356677312646	24.535916701289885	24.559386487112235	25.44434013428523
27	25.60607298010693	24.535975064879047	24.323319269929343	25.53463268508468
28	25.682791474353838	24.269198046595932	24.273083287675142	25.77492719137508
29	25.5244430877065	24.726231867395605	24.3121008250112	25.437224219886694
30	25.55346669447116	24.393210523102443	24.539263408201954	25.51405937422444
31	25.8070936066832	24.2725929121022	24.336579997335853	25.583733483878746
32	25.36051297624702	24.509332066803626	24.41521453168399	25.714940425265365
33	25.4334774293607	24.505310831940474	24.45757836212611	25.603633376572716
34	25.576501464892143	24.353445370821884	24.648247481535222	25.421805682750747
35	25.434725899284565	24.480949447231477	24.687974995183147	25.396349658300803
36	25.41038524635008	24.301593808402046	24.639924262186675	25.648096683061194
37	25.65115864174282	24.184510476341504	24.22930996887823	25.935020913037445
38	25.696737786820318	24.48400292102991	24.35222355869296	25.467035733456818
39	25.748897071649907	24.303117378217998	24.189970091691325	25.75801545844077
40	26.057250419443136	24.19703202357128	24.379240022962332	25.366477534023264
41	25.821491267715523	24.327337370815634	24.420266067863324	25.43090529360552
42	25.4556988121858	24.441537022059407	24.46104318315175	25.64172098260304
43	25.492347863182125	24.422927944393653	24.464953046859574	25.619771145564645
44	25.354003422284176	24.443165184681234	24.66423395863762	25.53859743439697
45	25.608479704656606	24.29898761156254	24.230717996536566	25.86181468724429
46	25.712314755346227	24.140441019053736	24.473306532822697	25.673937692777343
47	25.697100117428874	24.397849965389803	24.51321728939913	25.391832627782197
48	25.6908668633681	24.573062476360544	24.104436248991977	25.63163441127938
49	25.85461275210997	24.27130347078395	24.296280555300058	25.57780322180603
50	25.610720161753918	24.853806965726406	24.005391797331853	25.530081075187823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	12.0
2	13.0
3	42.5
4	72.0
5	79.0
6	86.0
7	92.5
8	99.0
9	123.0
10	147.0
11	234.5
12	322.0
13	658.0
14	994.0
15	1267.0
16	1540.0
17	1709.5
18	1879.0
19	1931.0
20	1983.0
21	2312.5
22	2642.0
23	3419.5
24	4197.0
25	5254.5
26	6312.0
27	7825.0
28	9338.0
29	10773.5
30	12209.0
31	15067.5
32	17926.0
33	21273.0
34	24620.0
35	29097.0
36	33574.0
37	38028.0
38	42482.0
39	45466.0
40	48450.0
41	52223.5
42	55997.0
43	59602.0
44	63207.0
45	68290.5
46	73374.0
47	77624.0
48	81874.0
49	86850.0
50	91826.0
51	93682.0
52	95538.0
53	96707.5
54	97877.0
55	97225.0
56	96573.0
57	93988.0
58	91403.0
59	86403.0
60	81403.0
61	72980.5
62	64558.0
63	57055.5
64	49553.0
65	42436.5
66	35320.0
67	29971.5
68	24623.0
69	20774.5
70	16926.0
71	13723.5
72	10521.0
73	9163.5
74	7806.0
75	6304.5
76	4803.0
77	4040.0
78	3277.0
79	2826.5
80	2376.0
81	1910.5
82	1445.0
83	1214.0
84	983.0
85	771.5
86	560.0
87	415.0
88	270.0
89	181.5
90	93.0
91	73.5
92	54.0
93	36.5
94	19.0
95	19.5
96	20.0
97	19.5
98	19.0
99	12.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02917858323706251
2	0.0038059021613559793
3	2.378688850847487E-4
4	1.585792567231658E-4
5	0.0
6	2.378688850847487E-4
7	0.0
8	0.0
9	0.0
10	7.92896283615829E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.92896283615829E-4
17	0.0
18	7.92896283615829E-5
19	0.0
20	2.378688850847487E-4
21	0.0
22	0.0
23	0.0
24	7.92896283615829E-4
25	3.171585134463316E-4
26	2.378688850847487E-4
27	4.757377701694974E-4
28	0.0012686340537853265
29	3.171585134463316E-4
30	9.514755403389948E-4
31	5.550273985310804E-4
32	7.92896283615829E-4
33	2.378688850847487E-4
34	0.0011100547970621607
35	1.585792567231658E-4
36	4.757377701694974E-4
37	0.0019029510806779896
38	0.0
39	8.721859119774119E-4
40	2.378688850847487E-4
41	0.0013479236821469095
42	0.004678088073333392
43	0.0038059021613559793
44	0.004202350303163894
45	0.0018236614523164068
46	0.0022993992224859044
47	7.92896283615829E-4
48	0.005233115471864472
49	0.0034094540195480647
50	0.0019029510806779896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1261199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.84545547767471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.75972716829029	27.696430950388994
2	17.04000255670557	15.283333519924055
3	7.8605653238544	10.575318967807986
4	4.300503014655356	7.714320659013306
5	2.5346872961839377	5.6834603145422244
6	1.6914834972938397	4.551320872146744
7	1.103256216190669	3.4633219266552553
8	0.7763984385164019	2.7854353285938775
9	0.5652888160792919	2.28155709901602
>10	2.2815683052281206	16.659065388590808
>50	0.06895727824268756	2.069300705756495
>100	0.017562088759346093	1.2371342675641726
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3786888508474873E-4
2	0.0	0.0	0.0	0.0	2.3786888508474873E-4
3	0.0	0.0	0.0	0.0	2.3786888508474873E-4
4	0.0	0.0	0.0	0.0	2.3786888508474873E-4
5	0.0	0.0	0.0	0.0	2.3786888508474873E-4
6	7.92896283615829E-4	0.0	0.0	0.0	3.171585134463316E-4
7	7.92896283615829E-4	0.0	0.0	0.0	3.171585134463316E-4
8	7.92896283615829E-4	0.0	0.0	0.0	3.171585134463316E-4
9	7.92896283615829E-4	0.0	0.0	0.0	3.171585134463316E-4
10	7.92896283615829E-4	0.0	0.0	0.0	3.171585134463316E-4
11	7.92896283615829E-4	0.0	0.0	0.0	3.171585134463316E-4
12	7.92896283615829E-4	0.0	0.0	0.0	0.0010307651687005777
13	7.92896283615829E-4	0.0	0.0	0.0	0.0012686340537853265
14	9.514755403389949E-4	0.0	0.0	0.0	0.0012686340537853265
15	9.514755403389949E-4	0.0	0.0	7.92896283615829E-5	0.001585792567231658
16	0.001665082195593241	0.0	0.0	1.585792567231658E-4	0.0017443718239548238
17	0.001665082195593241	0.0	0.0	1.585792567231658E-4	0.0017443718239548238
18	0.001665082195593241	0.0	0.0	3.171585134463316E-4	0.001982240709039573
19	0.001665082195593241	0.0	0.0	3.964481418079145E-4	0.0020615303374011554
20	0.001665082195593241	0.0	0.0	3.964481418079145E-4	0.002537268107570653
21	0.0017443718239548238	0.0	0.0	3.964481418079145E-4	0.002616557735932236
22	0.0017443718239548238	0.0	0.0	6.343170268926632E-4	0.002616557735932236
23	0.0018236614523164068	0.0	0.0	7.92896283615829E-4	0.002616557735932236
24	0.0018236614523164068	0.0	0.0	0.0012686340537853265	0.002616557735932236
25	0.001982240709039573	0.0	0.0	0.0014272133105084923	0.002616557735932236
26	0.001982240709039573	0.0	0.0	0.0021408199657627384	0.002695847364293819
27	0.001982240709039573	0.0	0.0	0.002616557735932236	0.0027751369926554015
28	0.001982240709039573	0.0	0.0	0.003409454019548065	0.0027751369926554015
29	0.001982240709039573	0.0	0.0	0.005312405100226055	0.0027751369926554015
30	0.001982240709039573	0.0	0.0	0.007611804322711959	0.0027751369926554015
31	0.001982240709039573	0.0	0.0	0.012686340537853264	0.0027751369926554015
32	0.001982240709039573	0.0	0.0	0.019505248576949394	0.0028544266210169845
33	0.0020615303374011554	0.0	0.0	0.026244866987683944	0.0028544266210169845
34	0.0020615303374011554	0.0	0.0	0.032905195770056905	0.003171585134463316
35	0.0020615303374011554	0.0	0.0	0.04289568894361635	0.003171585134463316
36	0.0020615303374011554	0.0	0.0	0.05431339542768429	0.003171585134463316
37	0.0020615303374011554	0.0	0.0	0.07262929957920994	0.003171585134463316
38	0.0020615303374011554	0.0	0.0	0.09792269102655489	0.003171585134463316
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	625	0.0	24.297897	1
TATACTG	185	0.0	23.783098	5
GTATCAA	1085	0.0	23.530321	1
TAGGACG	365	0.0	23.50617	4
GTCTACG	75	2.0570878E-6	23.476229	1
TCCGCTA	65	4.9319665E-4	20.307913	38
TGTAGGA	575	0.0	19.513254	2
AGGACGT	470	0.0	18.254793	5
GTCCTAC	555	0.0	18.241663	1
TTAGGAC	290	0.0	17.447773	3
TCCTATA	115	4.2727406E-6	17.217577	2
GTACCGT	90	2.2159542E-4	17.110619	6
CTACAGT	540	0.0	17.110617	4
GTAGGAC	505	0.0	16.553978	3
ATCAACG	1580	0.0	16.29067	3
TATCAAC	1570	0.0	16.254951	2
TCAACGC	1570	0.0	16.254309	4
ACAATAC	150	1.8383616E-7	16.132868	3
CAACGCA	1605	0.0	16.03692	5
CTGTAGG	620	0.0	15.974248	1
>>END_MODULE