FastQCFastQC Report
Thu 2 Feb 2017
SRR4062136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203709
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15110.7417443510105101No Hit
TATCAACGCAGAGTACTTTTTTTTT9370.4599698589654851No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6260.3073011010804628No Hit
GGTATCAACGCAGAGTACTTTTTTT6250.30681020475285825No Hit
CCATAGGGTCTTCTCGTCTTATTAT6120.3004285524939988No Hit
CTGTAGGACGTGGAATATGGCAAGA4650.22826679233612654No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4530.22237603640487164No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4250.20863093923194362No Hit
GTATCAACGCAGAGTACATGGGGTG4130.20274018330068871No Hit
GTACATGGGGTGGTATCAACGCAAA4030.19783122002464298No Hit
GTCCTACAGTGGACATTTCTAAATT3600.17672267793764634No Hit
GTATTAGAGGCACTGCCTGCCCAGT3520.17279550731680976No Hit
GTCAGGATACCGCGGCCGTTAAACT3490.17132281833399604No Hit
GTCCTAAAGTGTGTATTTCTCATTT3380.16592295873034574No Hit
CTTTAGGACGTGAAATATGGCGAGG3020.1482506909365811No Hit
CCATTGGGATGTCCTGATCCAACAT2970.14579620929855822No Hit
GTTCATGCTAGTCCCTAATTAAGGA2960.14530531297095367No Hit
TATCAACGCAGAGTACATGGGGTGG2870.14088724602251249No Hit
ACCTATAACTTCTCTGTTAACCCAA2820.13843276438448962No Hit
CTTCTACACCATTGGGATGTCCTGA2690.13205111212563017No Hit
ATAAATAATCCACCTATAACTTCTC2620.12861483783239816No Hit
ATCCTGACCGTGCAAAGGTAGCATA2600.12763304517718904No Hit
GGTCAGGATACCGCGGCCGTTAAAC2540.12468766721156158No Hit
CCTATAACTTCTCTGTTAACCCAAC2540.12468766721156158No Hit
GCCTAAAGGAAAGATCCAAAAAGAT2460.12076049659072499No Hit
CACTATAAATAATCCACCTATAACT2450.12026960026312043No Hit
CTGTTAGTATGAGTAACAAGAATTC2420.11879691128030671No Hit
GGTATCAACGCAGAGTACATGGGGT2420.11879691128030671No Hit
CTCTAATACTTGTAATGCTAGAGGT2410.11830601495270213No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2340.11486974065947012No Hit
GATTAAACCTTGTACCTTTTGCATA2310.1133970516766564No Hit
GACTATAGGCAATAATCACACTATA2280.11192436269384269No Hit
GTTATATAATTTAAGCTCCATAGGG2280.11192436269384269No Hit
GTATCAACGCAGAGTACATGGGAGA2260.11094257003863353No Hit
CTGTTAACCCAACACCGGAATGCCT2220.10897898472821525No Hit
GATTAAAGATAAGAGACAGTTGGAC2220.10897898472821525No Hit
ATCGTAAATAGATAGAAACCGACCT2090.10259733246935579No Hit
ACGCAGAGTACATGGGGTGGTATCA2080.10210643614175122No Hit
ATCAACGCAGAGTACTTTTTTTTTT2060.10112464348654207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCCA307.686544E-419.00172215
TTATACG250.006019862418.997052
TATCACC402.749599E-416.622423
CTAGGAC402.749599E-416.622423
GACAGTG350.002160181816.287197
AGAAAGC350.002160181816.287198
GTCCTAT350.002163734816.2831861
CTACACC350.002163734816.2831864
GTTGATC456.722025E-414.77911715
CGACCTG456.722025E-414.77911710
CTATCAC456.734577E-414.7754842
CACTGTT801.268345E-714.2512917
CACCATT400.005255094714.2512917
CCCAGCA400.005263678714.2477872
CCTACAG2000.014.2477873
AATAAAG400.005263678714.2477873
GCTATCA551.9495709E-413.8160371
TCCTACA2200.013.8160372
ATACACT551.9495709E-413.8160374
ACACCAT500.001490727913.3012056
Produced by FastQC (version 0.11.5)