##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062136_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	203709
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.068308223986175	32.0	32.0	32.0	32.0	32.0
2	30.778311218453776	32.0	32.0	32.0	32.0	32.0
3	30.844704946762292	32.0	32.0	32.0	32.0	32.0
4	30.791815776426176	32.0	32.0	32.0	32.0	32.0
5	30.797814529549505	32.0	32.0	32.0	32.0	32.0
6	34.39220652990295	36.0	36.0	36.0	32.0	36.0
7	34.289835009744294	36.0	36.0	36.0	32.0	36.0
8	34.15606085150877	36.0	36.0	36.0	32.0	36.0
9	34.23553205798468	36.0	36.0	36.0	32.0	36.0
10	34.07397807657001	36.0	36.0	36.0	32.0	36.0
11	34.37617385584338	36.0	36.0	36.0	32.0	36.0
12	34.207634419686904	36.0	36.0	36.0	32.0	36.0
13	34.289653378103075	36.0	36.0	36.0	32.0	36.0
14	34.1973894133298	36.0	36.0	36.0	32.0	36.0
15	34.19073285912748	36.0	36.0	36.0	32.0	36.0
16	34.18623133980335	36.0	36.0	36.0	32.0	36.0
17	34.09522897859201	36.0	36.0	36.0	32.0	36.0
18	34.17592251692365	36.0	36.0	36.0	32.0	36.0
19	34.09638258496188	36.0	36.0	36.0	32.0	36.0
20	33.99468359277204	36.0	36.0	36.0	32.0	36.0
21	33.97777712324934	36.0	36.0	36.0	32.0	36.0
22	33.966373601559084	36.0	36.0	36.0	32.0	36.0
23	34.017534816822035	36.0	36.0	36.0	32.0	36.0
24	33.964635828559366	36.0	36.0	36.0	32.0	36.0
25	33.68627797495447	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	7.0
5	18.0
6	33.0
7	13.0
8	38.0
9	28.0
10	15.0
11	7.0
12	15.0
13	9.0
14	89.0
15	96.0
16	133.0
17	171.0
18	234.0
19	336.0
20	465.0
21	611.0
22	887.0
23	1161.0
24	1445.0
25	1848.0
26	2298.0
27	2903.0
28	3758.0
29	4883.0
30	6292.0
31	8650.0
32	12379.0
33	18049.0
34	41202.0
35	95636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64009229032179	21.073120443778993	12.477847868240838	23.808939397658378
2	15.290663996151316	20.432387853082385	40.815194446898964	23.461753703867338
3	18.25157214179467	25.54307005787838	29.79828475771098	26.407073042615963
4	11.33133349371327	17.273901111040193	38.00955406194921	33.385211333297335
5	12.427935846944086	39.40521906520394	33.871870672467814	14.294974415384162
6	29.843839801938373	39.15450477224386	17.501338586158283	13.500316839659485
7	28.238925092639004	33.16279892666529	19.88126480504035	18.717011175655355
8	26.202470058040955	35.41186473164043	18.591781872152627	19.793883338165983
9	26.01741899968544	15.376789084617803	21.24193928908462	37.36385262661214
10	16.995703512894377	26.779306073089437	30.757735151556865	25.46725526245932
11	33.42117036286854	23.94312880087706	23.707148721528757	18.92855211472565
12	24.13691397897034	26.531384722775247	29.001558298554276	20.330142999700136
13	27.757323121844752	21.9892740044536	27.272637896879043	22.980764976822606
14	21.416986139781773	23.441954192470266	26.542809397424556	28.598250270323405
15	24.305005923733773	29.421047198147665	23.73819554712195	22.53575133099661
16	22.759691903874796	29.02827819094292	25.55605255526118	22.65597734992111
17	19.831684093065324	29.037443407217335	27.443751321112735	23.68712117860461
18	20.344131046989958	27.525077530238022	30.142183821614104	21.988607601157916
19	23.90246778901436	26.47394128804631	28.0021817967391	21.621409126200234
20	23.743421666855028	26.985047344343492	28.126028824278045	21.145502164523435
21	24.237510503117768	24.921503014579063	27.523819351288136	23.317167131015033
22	23.298704617380537	27.50378393253789	27.25954828691054	21.937963163171034
23	22.540421831473758	27.492249718729887	28.48222225497566	21.4851061948207
24	22.91321674438717	27.85800979818876	27.84621659205826	21.38255686536581
25	22.593262313770197	27.39887574197099	28.592417154762373	21.415444789496444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	259.0
1	259.0
2	238.0
3	217.0
4	217.0
5	217.0
6	566.5
7	916.0
8	916.0
9	916.0
10	938.5
11	961.0
12	961.0
13	961.0
14	1025.0
15	1089.0
16	1089.0
17	1089.0
18	1877.0
19	2665.0
20	2665.0
21	2665.0
22	4317.5
23	5970.0
24	5970.0
25	5970.0
26	8739.0
27	11508.0
28	11508.0
29	11508.0
30	14188.0
31	16868.0
32	16868.0
33	16868.0
34	18124.0
35	19380.0
36	19380.0
37	19380.0
38	19440.0
39	19500.0
40	19500.0
41	19500.0
42	19492.5
43	19485.0
44	19485.0
45	19485.0
46	22163.0
47	24841.0
48	24841.0
49	24841.0
50	24086.0
51	23331.0
52	23331.0
53	23331.0
54	20559.0
55	17787.0
56	17787.0
57	17787.0
58	15639.5
59	13492.0
60	13492.0
61	13492.0
62	11645.0
63	9798.0
64	9798.0
65	9798.0
66	8311.5
67	6825.0
68	6825.0
69	6825.0
70	5564.5
71	4304.0
72	4304.0
73	4304.0
74	3460.5
75	2617.0
76	2617.0
77	2617.0
78	1859.5
79	1102.0
80	1102.0
81	1102.0
82	772.5
83	443.0
84	443.0
85	443.0
86	319.0
87	195.0
88	195.0
89	195.0
90	123.0
91	51.0
92	51.0
93	51.0
94	35.0
95	19.0
96	19.0
97	19.0
98	52.5
99	86.0
100	86.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001963585310418293
2	0.0014726889828137195
3	0.002945377965627439
4	0.012763304517718902
5	0.0348536392599247
6	0.06676190055422195
7	0.1133970516766564
8	0.11388794800426098
9	0.12419677088395702
10	0.14088724602251249
11	0.1482506909365811
12	0.13843276438448962
13	0.13646917907407136
14	0.12223318557353871
15	0.14235993500532623
16	0.13057842314281648
17	0.13843276438448962
18	0.11781511862509757
19	0.10161553981414666
20	0.09817926552091465
21	0.0971974728657055
22	0.10652450309019239
23	0.0824705830375683
24	0.09916105817612379
25	0.09670657653810091
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	203709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.42089451128816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.14516462029852	27.14067616060164
2	15.890018338373348	12.845775100756471
3	6.538662391761091	7.928957483469066
4	3.166101941924436	5.11906690426049
5	1.7986179424587996	3.6350873059118647
6	1.146451949818438	2.7804367995523025
7	0.7505373993514775	2.1236175132173836
8	0.5355776587605203	1.731882243788934
9	0.42506163393692087	1.5463234319544055
>10	2.2479688126206874	17.185789533108505
>50	0.23196220594843397	6.617773392437251
>100	0.1178027956910895	9.228360062638371
>500	0.004857847244993381	1.374509717292805
>1k	0.0012144618112483452	0.7417443510105101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1511	0.7417443510105101	No Hit
TATCAACGCAGAGTACTTTTTTTTT	937	0.4599698589654851	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	626	0.3073011010804628	No Hit
GGTATCAACGCAGAGTACTTTTTTT	625	0.30681020475285825	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	612	0.3004285524939988	No Hit
CTGTAGGACGTGGAATATGGCAAGA	465	0.22826679233612654	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	453	0.22237603640487164	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	425	0.20863093923194362	No Hit
GTATCAACGCAGAGTACATGGGGTG	413	0.20274018330068871	No Hit
GTACATGGGGTGGTATCAACGCAAA	403	0.19783122002464298	No Hit
GTCCTACAGTGGACATTTCTAAATT	360	0.17672267793764634	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	352	0.17279550731680976	No Hit
GTCAGGATACCGCGGCCGTTAAACT	349	0.17132281833399604	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	338	0.16592295873034574	No Hit
CTTTAGGACGTGAAATATGGCGAGG	302	0.1482506909365811	No Hit
CCATTGGGATGTCCTGATCCAACAT	297	0.14579620929855822	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	296	0.14530531297095367	No Hit
TATCAACGCAGAGTACATGGGGTGG	287	0.14088724602251249	No Hit
ACCTATAACTTCTCTGTTAACCCAA	282	0.13843276438448962	No Hit
CTTCTACACCATTGGGATGTCCTGA	269	0.13205111212563017	No Hit
ATAAATAATCCACCTATAACTTCTC	262	0.12861483783239816	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	260	0.12763304517718904	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	254	0.12468766721156158	No Hit
CCTATAACTTCTCTGTTAACCCAAC	254	0.12468766721156158	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	246	0.12076049659072499	No Hit
CACTATAAATAATCCACCTATAACT	245	0.12026960026312043	No Hit
CTGTTAGTATGAGTAACAAGAATTC	242	0.11879691128030671	No Hit
GGTATCAACGCAGAGTACATGGGGT	242	0.11879691128030671	No Hit
CTCTAATACTTGTAATGCTAGAGGT	241	0.11830601495270213	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	234	0.11486974065947012	No Hit
GATTAAACCTTGTACCTTTTGCATA	231	0.1133970516766564	No Hit
GACTATAGGCAATAATCACACTATA	228	0.11192436269384269	No Hit
GTTATATAATTTAAGCTCCATAGGG	228	0.11192436269384269	No Hit
GTATCAACGCAGAGTACATGGGAGA	226	0.11094257003863353	No Hit
CTGTTAACCCAACACCGGAATGCCT	222	0.10897898472821525	No Hit
GATTAAAGATAAGAGACAGTTGGAC	222	0.10897898472821525	No Hit
ATCGTAAATAGATAGAAACCGACCT	209	0.10259733246935579	No Hit
ACGCAGAGTACATGGGGTGGTATCA	208	0.10210643614175122	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	206	0.10112464348654207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCCA	30	7.686544E-4	19.001722	15
TTATACG	25	0.0060198624	18.99705	2
TATCACC	40	2.749599E-4	16.62242	3
CTAGGAC	40	2.749599E-4	16.62242	3
GACAGTG	35	0.0021601818	16.28719	7
AGAAAGC	35	0.0021601818	16.28719	8
GTCCTAT	35	0.0021637348	16.283186	1
CTACACC	35	0.0021637348	16.283186	4
GTTGATC	45	6.722025E-4	14.779117	15
CGACCTG	45	6.722025E-4	14.779117	10
CTATCAC	45	6.734577E-4	14.775484	2
CACTGTT	80	1.268345E-7	14.251291	7
CACCATT	40	0.0052550947	14.251291	7
CCCAGCA	40	0.0052636787	14.247787	2
CCTACAG	200	0.0	14.247787	3
AATAAAG	40	0.0052636787	14.247787	3
GCTATCA	55	1.9495709E-4	13.816037	1
TCCTACA	220	0.0	13.816037	2
ATACACT	55	1.9495709E-4	13.816037	4
ACACCAT	50	0.0014907279	13.301205	6
>>END_MODULE