FastQCFastQC Report
Thu 2 Feb 2017
SRR4062136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203709
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG5680.2788291140793976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.19537673838662012No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT3400.16690475138555488No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC3170.1556141358506497No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA2810.13794186805688508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.13499649009125764No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA2700.13254200845323477No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2690.13205111212563017No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC2680.13156021579802563No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2680.13156021579802563No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2570.1261603561943753No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC2540.12468766721156158No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG2510.12321497822874788No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC2500.1227240819011433No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2480.12174228924593415No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG2440.11977870393551585No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT2440.11977870393551585No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT2420.11879691128030671No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC2320.11388794800426098No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA2290.11241525902144725No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA2250.11045167371102897No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC2230.10946988105581983No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT2200.1079971920730061No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT2180.10701539941779695No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC2140.10505181410737865No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA2050.10063374715893751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTTG250.002349053535.1974455
TAAATTC751.8189894E-1235.19744516
CTGGACT250.002349053535.1974454
AAATTCA701.02591E-931.42629417
ATTGAAC701.02591E-931.4262948
ATACATG801.2914825E-1030.2478081
CACAAAC300.005729100729.33840841
ACATAAA454.048364E-529.33840826
TCTGGAC300.005735981329.3312073
CTGTGCT300.005735981329.3312079
GTCCTAT407.014914E-427.4980071
GTTTCCA1006.002665E-1126.40456625
GTCCTAC2000.026.3980851
ACAATGC508.31215E-526.39808516
TTGGTGT508.31215E-526.39808534
CTAATAC756.9574526E-826.3980853
AATTCAT858.1563485E-925.88047618
ATTCATT959.513315E-1025.47183619
AACGAAT701.125265E-625.14103531
AGTGTAT701.125265E-625.14103510
Produced by FastQC (version 0.11.5)