##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	203709
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.098208719300573	32.0	32.0	32.0	32.0	32.0
2	31.43903313059315	32.0	32.0	32.0	32.0	32.0
3	31.567245433436913	32.0	32.0	32.0	32.0	32.0
4	31.67459464235748	32.0	32.0	32.0	32.0	32.0
5	31.652877388824255	32.0	32.0	32.0	32.0	32.0
6	35.28500949884394	36.0	36.0	36.0	36.0	36.0
7	35.28273664884713	36.0	36.0	36.0	36.0	36.0
8	35.2366120299054	36.0	36.0	36.0	36.0	36.0
9	35.289368658233066	36.0	36.0	36.0	36.0	36.0
10	35.18901472198087	36.0	36.0	36.0	36.0	36.0
11	35.31345203206535	36.0	36.0	36.0	36.0	36.0
12	35.2444614621838	36.0	36.0	36.0	36.0	36.0
13	35.26997334432941	36.0	36.0	36.0	36.0	36.0
14	35.22141878856604	36.0	36.0	36.0	36.0	36.0
15	35.24259605613891	36.0	36.0	36.0	36.0	36.0
16	35.235055888546896	36.0	36.0	36.0	36.0	36.0
17	35.20308871969329	36.0	36.0	36.0	36.0	36.0
18	35.22054008413963	36.0	36.0	36.0	36.0	36.0
19	35.17528926066104	36.0	36.0	36.0	36.0	36.0
20	35.13363670726379	36.0	36.0	36.0	36.0	36.0
21	35.107467024039195	36.0	36.0	36.0	36.0	36.0
22	35.106804313996925	36.0	36.0	36.0	36.0	36.0
23	35.12713723988631	36.0	36.0	36.0	36.0	36.0
24	35.10140936335655	36.0	36.0	36.0	36.0	36.0
25	35.104487283330634	36.0	36.0	36.0	36.0	36.0
26	35.08654011359341	36.0	36.0	36.0	36.0	36.0
27	35.06111659278677	36.0	36.0	36.0	36.0	36.0
28	35.03839791074523	36.0	36.0	36.0	36.0	36.0
29	34.996990805511786	36.0	36.0	36.0	36.0	36.0
30	34.97330996666814	36.0	36.0	36.0	36.0	36.0
31	34.971503468182554	36.0	36.0	36.0	36.0	36.0
32	34.92214875140519	36.0	36.0	36.0	36.0	36.0
33	34.89195862725751	36.0	36.0	36.0	36.0	36.0
34	34.86944612167356	36.0	36.0	36.0	36.0	36.0
35	34.85762042914157	36.0	36.0	36.0	36.0	36.0
36	34.81773019356042	36.0	36.0	36.0	32.0	36.0
37	34.77371151986412	36.0	36.0	36.0	32.0	36.0
38	34.74390920381525	36.0	36.0	36.0	32.0	36.0
39	34.664668718613314	36.0	36.0	36.0	32.0	36.0
40	34.6582576125748	36.0	36.0	36.0	32.0	36.0
41	34.61224099082515	36.0	36.0	36.0	32.0	36.0
42	34.566783990888965	36.0	36.0	36.0	32.0	36.0
43	34.54706468540909	36.0	36.0	36.0	32.0	36.0
44	34.42227392996873	36.0	36.0	36.0	32.0	36.0
45	34.35299864021717	36.0	36.0	36.0	32.0	36.0
46	34.20385942692763	36.0	36.0	36.0	32.0	36.0
47	34.14224702884998	36.0	36.0	36.0	32.0	36.0
48	34.071479414262505	36.0	36.0	36.0	32.0	36.0
49	34.0124393129415	36.0	36.0	36.0	32.0	36.0
50	33.610139954543	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	0.0
21	7.0
22	18.0
23	39.0
24	122.0
25	278.0
26	579.0
27	1135.0
28	2001.0
29	3125.0
30	4528.0
31	6513.0
32	9476.0
33	15140.0
34	31644.0
35	129102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.004336147163826	21.838369255096076	13.024646797978756	24.13264779976134
2	14.999729991114252	20.73177317289896	40.25930671536645	24.00919012062033
3	18.05034657450861	25.79967404323836	29.952186462976414	26.197792919276612
4	11.118365503563926	17.37339721562236	38.11779606102853	33.39044121978518
5	12.106976127711588	39.66147789248389	33.71230529824407	14.519240681560461
6	30.05905482821083	39.366940095921144	17.299677481112763	13.274327594755263
7	27.917765047199683	33.4158039163709	19.96769902164362	18.698732014785797
8	26.334624390674932	35.2085573047828	18.690877673544122	19.765940630998138
9	26.07984919664816	14.801015173605487	21.45216951631985	37.666966113426504
10	16.859834371579066	26.97180782390567	30.81061710577343	25.357740698741832
11	33.97689841882293	23.753000603802484	23.58118688914088	18.688914088233705
12	23.95034092749952	26.58989048102931	28.92557520777187	20.534193383699296
13	27.941328070924705	21.750143587175824	27.422941548974272	22.885586792925203
14	21.50175004540791	23.137907505313954	26.877555729005593	28.482786720272546
15	24.55561609943596	29.011972961430278	23.77754542018271	22.654865518951052
16	22.862739130221346	28.36966819991458	25.833672873293505	22.933919796570564
17	20.085514140268717	28.417006612373534	27.969800057925763	23.527679189431982
18	20.485695210791917	26.92285035442889	30.68166198676537	21.909792448013825
19	23.960158854051613	25.96203407802306	28.622692173639848	21.455114894285476
20	23.7549641891129	26.80441217619251	28.424860953615205	21.015762681079384
21	24.125590916454353	24.402456445223333	28.10676013332744	23.36519250499487
22	23.23608677083487	27.006661463165592	28.0159443127206	21.741307453278942
23	22.276875346695533	27.075386949030232	29.155805585418413	21.491932118855818
24	22.872557153166067	27.353011923988866	28.47373924312861	21.300691679716454
25	22.637684147484894	26.986043817406202	29.190168328350737	21.186103706758168
26	20.531059468056906	28.00359341403788	30.159150933207663	21.306196184697555
27	21.16853535452707	28.189369096942684	28.761757024760932	21.880338523769318
28	20.735580722915227	27.59114986033588	29.032463930329943	22.64080548641895
29	21.257983279906924	27.27790404846176	28.516447642938143	22.947665028693173
30	21.668483009827888	26.615318154595347	29.468940531943094	22.247258303633668
31	22.551397097806664	26.35880770514658	28.58356078308167	22.50623441396509
32	20.840021795796947	27.42490513428469	28.73608922531651	22.99898384460186
33	21.26121084488167	28.138177498294137	29.109170434295983	21.491441222528216
34	21.25581098527802	27.20154730522461	30.148888856162465	21.393752853334906
35	22.01473670775469	28.015453416392994	28.194139679641054	21.775670196211262
36	21.0177262663898	27.379251775817465	29.080698447294917	22.52232351049782
37	22.280911893494608	27.077974355449957	26.70145502385768	23.939658727197756
38	22.00835505549583	28.32422720645627	28.26433785448851	21.403079883559393
39	21.526785758115743	27.83725804947253	27.496085101787354	23.13987109062437
40	22.406962873510743	27.889293060198618	28.79892395524989	20.90482011104075
41	22.6037652487666	27.37389852973663	29.1622689673793	20.860067254117475
42	21.579148911101072	29.043850519411663	27.872474127604423	21.50452644188284
43	20.959356697872842	29.115017746773425	28.0320472854555	21.89357826989823
44	21.15716922593567	28.403958685151004	27.974904763774887	22.463967325138437
45	21.597023131602718	28.625849271491973	27.77755959627695	21.999568000628365
46	23.22328857907268	27.896222478584225	26.315505264966493	22.5649836773766
47	23.081907660587614	28.699835546501067	26.07447043518814	22.143786357723176
48	22.062346588119784	29.192439862542958	25.682866961217478	23.062346588119784
49	22.408612919379067	28.110793641442555	26.673343348355864	22.80725009082251
50	21.267414803687668	29.119417199297025	26.55739153485906	23.055776462156246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	63.0
2	18.0
3	41.0
4	64.0
5	66.5
6	69.0
7	59.0
8	49.0
9	92.0
10	135.0
11	217.0
12	299.0
13	575.0
14	851.0
15	1158.0
16	1465.0
17	1803.0
18	2141.0
19	2231.0
20	2321.0
21	2414.5
22	2508.0
23	2314.0
24	2120.0
25	2583.0
26	3046.0
27	4041.5
28	5037.0
29	5768.0
30	6499.0
31	6756.5
32	7014.0
33	8179.5
34	9345.0
35	10602.0
36	11859.0
37	13536.0
38	15213.0
39	14383.5
40	13554.0
41	13442.5
42	13331.0
43	11877.5
44	10424.0
45	10227.0
46	10030.0
47	9771.5
48	9513.0
49	9239.0
50	8965.0
51	9340.0
52	9715.0
53	9426.0
54	9137.0
55	8915.5
56	8694.0
57	8594.5
58	8495.0
59	7820.0
60	7145.0
61	6508.5
62	5872.0
63	5321.5
64	4771.0
65	4371.5
66	3972.0
67	3537.5
68	3103.0
69	2756.5
70	2410.0
71	2062.0
72	1714.0
73	1424.5
74	1135.0
75	889.0
76	643.0
77	535.5
78	428.0
79	343.0
80	258.0
81	196.0
82	134.0
83	95.0
84	56.0
85	40.5
86	25.0
87	17.5
88	10.0
89	5.0
90	0.0
91	2.5
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03534453558752927
2	0.005890755931254878
3	4.908963276045732E-4
4	4.908963276045732E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.817926552091465E-4
17	0.0
18	4.908963276045732E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	9.817926552091465E-4
25	0.0
26	0.0014726889828137195
27	4.908963276045732E-4
28	0.002945377965627439
29	9.817926552091465E-4
30	0.0014726889828137195
31	4.908963276045732E-4
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	4.908963276045732E-4
38	0.0
39	0.0
40	0.0
41	0.001963585310418293
42	0.008345237569277743
43	0.004908963276045732
44	0.002454481638022866
45	0.002454481638022866
46	0.001963585310418293
47	0.001963585310418293
48	0.004418066948441159
49	0.007363444914068598
50	0.0014726889828137195
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	203709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.44428081233524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04400410881739	30.24166826207973
2	15.811215304240257	14.05436185931893
3	6.304604747230414	8.406108713900712
4	3.1622430608480507	5.621744743727572
5	1.793741785126523	3.9860781801491343
6	1.09899819963109	2.930651075799302
7	0.7256701679976142	2.257632210653432
8	0.5025569656604483	1.7868626324806465
9	0.37111899002617715	1.4844704946762293
>10	1.9108209903133524	15.835824632195926
>50	0.17340976617294587	5.356660726821103
>100	0.10051139313208965	7.759107354117884
>500	0.0011045208036493366	0.2788291140793976
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	568	0.2788291140793976	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	398	0.19537673838662012	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	340	0.16690475138555488	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	317	0.1556141358506497	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	281	0.13794186805688508	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.13499649009125764	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	270	0.13254200845323477	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	269	0.13205111212563017	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	268	0.13156021579802563	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	268	0.13156021579802563	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	257	0.1261603561943753	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	254	0.12468766721156158	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	251	0.12321497822874788	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	250	0.1227240819011433	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	248	0.12174228924593415	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	244	0.11977870393551585	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	244	0.11977870393551585	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	242	0.11879691128030671	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	232	0.11388794800426098	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	229	0.11241525902144725	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	225	0.11045167371102897	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	223	0.10946988105581983	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	220	0.1079971920730061	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	218	0.10701539941779695	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	214	0.10505181410737865	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	205	0.10063374715893751	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	4.908963276045732E-4	0.0
23	0.0	0.0	0.0	0.0014726889828137195	0.0
24	0.0	0.0	0.0	0.001963585310418293	0.0
25	0.0	0.0	0.0	0.002454481638022866	0.0
26	0.0	0.0	0.0	0.0034362742932320125	0.0
27	0.0	0.0	0.0	0.003927170620836586	0.0
28	0.0	0.0	0.0	0.004908963276045732	0.0
29	0.0	0.0	0.0	0.008836133896882318	0.0
30	0.0	0.0	0.0	0.014726889828137196	0.0
31	0.0	0.0	0.0	0.029453779656274392	0.0
32	0.0	0.0	0.0	0.04516246213962073	0.0
33	0.0	0.0	0.0	0.058907559312548784	0.0
34	0.0	0.0	0.0	0.07756161976152257	0.0
35	0.0	0.0	0.0	0.10210643614175122	0.0
36	0.0	0.0	0.0	0.13794186805688508	0.0
37	0.0	0.0	0.0	0.17524998895483263	0.0
38	0.0	0.0	0.0	0.21697617680122136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGTTG	25	0.0023490535	35.197445	5
TAAATTC	75	1.8189894E-12	35.197445	16
CTGGACT	25	0.0023490535	35.197445	4
AAATTCA	70	1.02591E-9	31.426294	17
ATTGAAC	70	1.02591E-9	31.426294	8
ATACATG	80	1.2914825E-10	30.247808	1
CACAAAC	30	0.0057291007	29.338408	41
ACATAAA	45	4.048364E-5	29.338408	26
TCTGGAC	30	0.0057359813	29.331207	3
CTGTGCT	30	0.0057359813	29.331207	9
GTCCTAT	40	7.014914E-4	27.498007	1
GTTTCCA	100	6.002665E-11	26.404566	25
GTCCTAC	200	0.0	26.398085	1
ACAATGC	50	8.31215E-5	26.398085	16
TTGGTGT	50	8.31215E-5	26.398085	34
CTAATAC	75	6.9574526E-8	26.398085	3
AATTCAT	85	8.1563485E-9	25.880476	18
ATTCATT	95	9.513315E-10	25.471836	19
AACGAAT	70	1.125265E-6	25.141035	31
AGTGTAT	70	1.125265E-6	25.141035	10
>>END_MODULE